##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063095_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	855845
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189494593062996	32.0	32.0	32.0	32.0	32.0
2	31.310660224690217	32.0	32.0	32.0	32.0	32.0
3	31.40022200281593	32.0	32.0	32.0	32.0	32.0
4	31.48935029123264	32.0	32.0	32.0	32.0	32.0
5	31.419262833807522	32.0	32.0	32.0	32.0	32.0
6	34.95277883261572	36.0	36.0	36.0	36.0	36.0
7	34.98292681501908	36.0	36.0	36.0	36.0	36.0
8	34.919966816421194	36.0	36.0	36.0	36.0	36.0
9	35.02063574595867	36.0	36.0	36.0	36.0	36.0
10	34.88010212129533	36.0	36.0	36.0	32.0	36.0
11	35.05223843102431	36.0	36.0	36.0	36.0	36.0
12	34.961788641634875	36.0	36.0	36.0	36.0	36.0
13	35.00953677359802	36.0	36.0	36.0	36.0	36.0
14	34.94873954980166	36.0	36.0	36.0	32.0	36.0
15	34.91698146276487	36.0	36.0	36.0	32.0	36.0
16	34.93880433957084	36.0	36.0	36.0	36.0	36.0
17	34.89786234656977	36.0	36.0	36.0	32.0	36.0
18	34.89604192347913	36.0	36.0	36.0	32.0	36.0
19	34.8827311020103	36.0	36.0	36.0	32.0	36.0
20	34.87165549836711	36.0	36.0	36.0	32.0	36.0
21	34.86064649556871	36.0	36.0	36.0	32.0	36.0
22	34.84788367052445	36.0	36.0	36.0	32.0	36.0
23	34.79026108699589	36.0	36.0	36.0	32.0	36.0
24	34.78616104551642	36.0	36.0	36.0	32.0	36.0
25	34.75055997289229	36.0	36.0	36.0	32.0	36.0
26	34.69901792964848	36.0	36.0	36.0	32.0	36.0
27	34.67871168260608	36.0	36.0	36.0	32.0	36.0
28	34.653697807430085	36.0	36.0	36.0	32.0	36.0
29	34.613563203617474	36.0	36.0	36.0	32.0	36.0
30	34.59279892971274	36.0	36.0	36.0	32.0	36.0
31	34.58284151920032	36.0	36.0	36.0	32.0	36.0
32	34.5557969024765	36.0	36.0	36.0	32.0	36.0
33	34.51197004130421	36.0	36.0	36.0	32.0	36.0
34	34.495741635459694	36.0	36.0	36.0	32.0	36.0
35	34.45838323528209	36.0	36.0	36.0	32.0	36.0
36	34.425020885791234	36.0	36.0	36.0	32.0	36.0
37	34.41388452348264	36.0	36.0	36.0	32.0	36.0
38	33.99631942699905	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	18.0
22	59.0
23	166.0
24	507.0
25	1324.0
26	2745.0
27	5420.0
28	9730.0
29	15175.0
30	22811.0
31	33282.0
32	48436.0
33	77445.0
34	191604.0
35	447118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01444425098712	18.37777074060528	12.052728936295022	26.55505607211258
2	15.32695756825126	21.213070123678936	37.31037746320888	26.14959484486093
3	17.698648703912507	25.600546827988712	28.983402368419515	27.717402099679266
4	11.959085953849012	16.537495779015543	36.66455179279377	34.83886647434167
5	13.781850175791588	37.457571073199176	33.51689503799178	15.24368371301746
6	33.110434716566665	36.509648359223924	17.091412580549047	13.28850434366036
7	29.186895259995115	31.018773303047404	21.713795637751314	18.080535799206164
8	27.6935318779007	33.06762573788191	19.975158559897547	19.26368382431984
9	27.793571726533717	13.961227511150215	18.590743538600137	39.65445722371593
10	15.44499711977865	27.106563516183574	32.29750524924723	25.15093411479055
11	36.97386394575311	21.440865818874	22.578176213584857	19.007094021788028
12	24.491291042866507	24.231780828960453	29.255716293759487	22.021211834413553
13	29.693495543580372	19.931903477736597	25.243969695411785	25.13063128327125
14	23.581022264545563	19.77402450210026	25.873259760821178	30.771693472532995
15	24.90193901933177	27.045785159695974	23.108156266613697	24.944119554358558
16	25.132150251681377	25.94912156888405	24.283631129037538	24.635097050397032
17	23.76189613773522	25.904573842226103	25.73678645081761	24.596743569221065
18	24.645233657963768	24.785445962761948	26.851707961137823	23.71761241813646
19	25.17815725978419	25.1771056674982	26.282562847244535	23.36217422547307
20	25.523897434699037	24.084851813120366	25.915790826609957	24.475459925570636
21	26.67363833404413	24.348918320490277	24.962580841156985	24.014862504308606
22	25.370481804532364	24.610998486875545	25.546214559879417	24.472305148712675
23	24.075093212189618	24.26554870461054	26.040173256076173	25.619184827123664
24	24.31047678025811	25.261583581139107	25.944066974744256	24.483872663858524
25	24.555264095718265	24.565546331403468	25.908312837020723	24.970876735857544
26	24.258977103303874	25.376704165712443	26.255602656558906	24.10871607442478
27	25.06519879791177	24.989133533681372	25.37845682157029	24.567210846836574
28	24.174587688191203	24.811502082736943	26.311072682553498	24.702837546518353
29	24.27133417850195	25.040398670319973	26.191191161951053	24.49707598922702
30	24.18732363921037	25.21063977706244	26.378024058094628	24.224012525632563
31	24.499412860973656	25.03934707803399	25.49433600710409	24.96690405388826
32	24.186272046924383	25.24382335586467	25.43906899029614	25.130835606914804
33	24.012759319736634	24.776916380886725	25.97888636376914	25.2314379356075
34	24.576997002961985	24.89866739888648	26.20696504624085	24.317370551910685
35	25.317668503058382	24.761376183771596	25.938692169726995	23.98226314344303
36	24.283252224409793	25.410442311399844	25.75816882729934	24.548136636891027
37	25.18283100327746	25.344191997382705	25.470383071701068	24.002593927638767
38	24.21221132331205	25.185401562198763	25.94371644398226	24.658670670506925
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	131.0
1	131.5
2	132.0
3	132.0
4	474.5
5	817.0
6	817.0
7	965.0
8	1113.0
9	1123.5
10	1134.0
11	1134.0
12	1476.0
13	1818.0
14	2239.5
15	2661.0
16	2661.0
17	3954.5
18	5248.0
19	5248.0
20	6135.0
21	7022.0
22	7209.5
23	7397.0
24	7397.0
25	8406.5
26	9416.0
27	9416.0
28	12064.5
29	14713.0
30	17691.5
31	20670.0
32	20670.0
33	25761.5
34	30853.0
35	30853.0
36	34538.0
37	38223.0
38	43122.5
39	48022.0
40	48022.0
41	51828.5
42	55635.0
43	62044.0
44	68453.0
45	68453.0
46	71955.5
47	75458.0
48	75458.0
49	78337.5
50	81217.0
51	80200.0
52	79183.0
53	79183.0
54	75355.5
55	71528.0
56	71528.0
57	68700.5
58	65873.0
59	59002.5
60	52132.0
61	52132.0
62	48186.0
63	44240.0
64	36310.5
65	28381.0
66	28381.0
67	23258.0
68	18135.0
69	18135.0
70	14647.0
71	11159.0
72	8641.5
73	6124.0
74	6124.0
75	4553.5
76	2983.0
77	2983.0
78	2809.5
79	2636.0
80	2011.5
81	1387.0
82	1387.0
83	1342.5
84	1298.0
85	1298.0
86	827.0
87	356.0
88	284.5
89	213.0
90	213.0
91	129.5
92	46.0
93	31.5
94	17.0
95	17.0
96	14.5
97	12.0
98	12.0
99	10.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008412738287890915
2	0.0
3	0.0
4	2.336871746636365E-4
5	2.336871746636365E-4
6	0.0
7	2.336871746636365E-4
8	0.0019863409846409105
9	0.003738994794618184
10	9.34748698654546E-4
11	0.006309553715918186
12	2.336871746636365E-4
13	1.1684358733181825E-4
14	0.0
15	0.0
16	1.1684358733181825E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.336871746636365E-4
24	0.0
25	0.0
26	1.1684358733181825E-4
27	1.1684358733181825E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	855845.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.50409506746326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.748274868647	48.85601299186273
2	12.520901642967793	15.151316466729606
3	3.278188151288297	5.950314226637364
4	1.23041226282004	2.977799220873452
5	0.617561489175392	1.868249952553605
6	0.36178215929196517	1.3133581295707917
7	0.22498416691023537	0.9528724396387633
8	0.1561963847281492	0.7560416728628806
9	0.12125273687574871	0.6602658407208367
>10	0.5927381090292723	6.665689848617622
>50	0.06889304027112303	2.9342765172067113
>100	0.07239302603195777	8.590009052130252
>500	0.005643542331126675	2.3272089449278255
>1k	7.784196318795416E-4	0.9965846956675493
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3449	0.4029935327074412	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2423	0.28311201210499565	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1474	0.1722274477271001	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1118	0.13063113063697282	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	939	0.10971612850457733	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	932	0.10889822339325461	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	879	0.10270551326466826	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.3368717466363653E-4	0.0
10	0.0	0.0	0.0	2.3368717466363653E-4	0.0
11	0.0	0.0	0.0	2.3368717466363653E-4	0.0
12	0.0	0.0	0.0	3.505307619954548E-4	1.1684358733181826E-4
13	0.0	0.0	0.0	3.505307619954548E-4	1.1684358733181826E-4
14	0.0	0.0	0.0	5.842179366590913E-4	1.1684358733181826E-4
15	0.0	0.0	0.0	5.842179366590913E-4	2.3368717466363653E-4
16	0.0	0.0	0.0	5.842179366590913E-4	2.3368717466363653E-4
17	0.0	0.0	0.0	7.010615239909096E-4	2.3368717466363653E-4
18	0.0	0.0	0.0	8.179051113227278E-4	2.3368717466363653E-4
19	0.0	0.0	0.0	8.179051113227278E-4	2.3368717466363653E-4
20	0.0	0.0	0.0	9.347486986545461E-4	2.3368717466363653E-4
21	0.0	0.0	0.0	0.0011684358733181825	2.3368717466363653E-4
22	0.0	0.0	0.0	0.001752653809977274	2.3368717466363653E-4
23	0.0	0.0	0.0	0.0024537153339681834	2.3368717466363653E-4
24	0.0	0.0	0.0	0.0037389947946181844	2.3368717466363653E-4
25	0.0	0.0	0.0	0.004556899905940912	2.3368717466363653E-4
26	0.0	0.0	0.0	0.005842179366590913	2.3368717466363653E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	50	7.193618E-4	19.200104	2
TCTATGC	85	2.3911525E-7	18.825832	3
ATAAGAC	100	1.3117333E-6	16.001959	3
ATACCGT	215	0.0	15.630733	6
TACCGTC	205	0.0	15.612578	7
ACCGTCG	195	0.0	15.592562	8
TATTACA	135	7.898052E-9	15.407493	2
TCTTATA	115	3.341629E-7	15.304432	2
CGTTTAT	150	1.9699655E-9	14.932544	31
CGTCGTA	215	0.0	14.883802	10
CTATTAG	65	0.004160273	14.769312	1
CCGTCGT	220	0.0	14.547235	9
TCGTTTA	165	4.838512E-10	14.544684	30
AACCGCG	135	1.314238E-7	14.224793	7
GTCGTAG	240	0.0	14.000077	11
ATGGTCG	150	3.1574018E-8	13.869174	5
CCGATAA	150	3.1597665E-8	13.868364	9
TATACTG	105	3.674357E-5	13.716766	5
CTATACT	105	3.6763777E-5	13.715963	4
ATTACAC	105	3.6763777E-5	13.715963	3
>>END_MODULE