FastQCFastQC Report
Thu 2 Feb 2017
SRR4063094_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063094_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896648
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT18330.20442804757273758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT12780.14253084822583667No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11850.13215888509203164No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11710.1305975142976954No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11560.12892461701804944No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10880.12134081601698772No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10840.12089471007574878No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10560.11777196848707633No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9970.11119190585380218No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9950.1109688528831827No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9420.10505794916176693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT400.004488922719.9952266
GCTCTAA659.340794E-619.7139931
GTCCTAC2800.019.4499661
GGTCTAT601.12826296E-418.6872221
GTATTAC701.7696957E-518.3058511
CTAGCGG1950.018.04999229
CGCGGTC1352.3646862E-1117.77749810
AACCGCG1352.3646862E-1117.7755177
CGTAAAC550.001364981517.45329932
TCCTACA3550.017.1303162
TAGCGGC2000.016.798830
TCTAGCG2100.016.76070628
TAGGACG2500.016.6360264
CGGTCCT1354.4201443E-1016.59233312
GAACCGC1551.0913936E-1116.5121866
CGACCCG1651.8189894E-1216.4809135
GTCGTAA600.00244250815.99885730
CTACAGT4850.015.5014534
ATAGTAT1255.1470124E-815.3563336
AGGACGT2650.015.0907355
Produced by FastQC (version 0.11.5)