##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063094_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	896648
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24726760110991	32.0	32.0	32.0	32.0	32.0
2	30.872859806746906	32.0	32.0	32.0	32.0	32.0
3	30.92197495561246	32.0	32.0	32.0	32.0	32.0
4	30.988249569507765	32.0	32.0	32.0	32.0	32.0
5	30.9031938954863	32.0	32.0	32.0	32.0	32.0
6	34.559507186766716	36.0	36.0	36.0	32.0	36.0
7	34.49308981897021	36.0	36.0	36.0	32.0	36.0
8	34.485057681498205	36.0	36.0	36.0	32.0	36.0
9	34.60074410470999	36.0	36.0	36.0	32.0	36.0
10	34.33895463994789	36.0	36.0	36.0	32.0	36.0
11	34.59457445954265	36.0	36.0	36.0	32.0	36.0
12	34.40812894246125	36.0	36.0	36.0	32.0	36.0
13	34.501692972047	36.0	36.0	36.0	32.0	36.0
14	34.39979456821406	36.0	36.0	36.0	32.0	36.0
15	34.34609121974287	36.0	36.0	36.0	32.0	36.0
16	34.35482374354262	36.0	36.0	36.0	32.0	36.0
17	34.29257523576699	36.0	36.0	36.0	32.0	36.0
18	34.31140090648727	36.0	36.0	36.0	32.0	36.0
19	34.291703098651865	36.0	36.0	36.0	32.0	36.0
20	34.2807143940543	36.0	36.0	36.0	32.0	36.0
21	34.26193779498755	36.0	36.0	36.0	32.0	36.0
22	34.234887045975675	36.0	36.0	36.0	32.0	36.0
23	34.187503903426986	36.0	36.0	36.0	32.0	36.0
24	34.16143235695613	36.0	36.0	36.0	32.0	36.0
25	34.14072077336926	36.0	36.0	36.0	32.0	36.0
26	34.08871374273963	36.0	36.0	36.0	32.0	36.0
27	34.085901044780115	36.0	36.0	36.0	32.0	36.0
28	34.05464686253691	36.0	36.0	36.0	32.0	36.0
29	34.032513316262346	36.0	36.0	36.0	32.0	36.0
30	34.00117771968487	36.0	36.0	36.0	32.0	36.0
31	34.02034800724476	36.0	36.0	36.0	32.0	36.0
32	33.97607310784165	36.0	36.0	36.0	32.0	36.0
33	33.93804926794015	36.0	36.0	36.0	32.0	36.0
34	33.94946958003587	36.0	36.0	36.0	32.0	36.0
35	33.90640362773352	36.0	36.0	36.0	32.0	36.0
36	33.870894710075746	36.0	36.0	36.0	32.0	36.0
37	33.86014132636218	36.0	36.0	36.0	32.0	36.0
38	33.26772378904542	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	11.0
11	16.0
12	2.0
13	3.0
14	118.0
15	231.0
16	337.0
17	383.0
18	424.0
19	581.0
20	800.0
21	1108.0
22	1669.0
23	2628.0
24	4036.0
25	5853.0
26	8656.0
27	12488.0
28	17460.0
29	23293.0
30	31340.0
31	42728.0
32	57057.0
33	85183.0
34	190826.0
35	409413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.576198547918466	17.208461468027185	11.139287716265681	26.07605226778867
2	16.80204614768285	19.629671373237827	37.52766082334679	26.040621655732526
3	19.001154444611757	23.64380643254307	28.591744884471883	28.763294238373295
4	12.429750296389898	15.224681945477641	36.44095679008903	35.90461096804343
5	14.44945201155867	36.59527816533448	33.89174955918911	15.063520263917745
6	34.19037815327548	35.6570645973276	16.882786648480394	13.269770600916525
7	30.18486630204941	30.359405251559142	21.067576127978874	18.388152318412576
8	27.972336816324894	33.137491733053174	19.711970092890926	19.178201357731
9	27.613065550807004	13.954639947894826	18.625257626181067	39.8070368751171
10	15.752963271934012	26.581787197594586	31.858574863602502	25.8066746668689
11	37.78792694827827	20.812045169385193	22.343343092151123	19.056684790185418
12	24.838728385588606	23.634990809971626	28.928402541087454	22.597878263352307
13	29.822491015238956	19.11896916737868	25.24299567883746	25.815544138544904
14	23.419395999803694	20.01615069220439	24.93162724916235	31.632826058829565
15	25.20428140355966	27.39919793403872	22.213880537908494	25.182640124493133
16	25.74907338314701	25.37619584603493	24.143681103262427	24.731049667555638
17	24.036905201464876	25.77206542630505	25.452920880932645	24.738108491297428
18	25.163536834685313	24.35669934986789	26.5852616645122	23.8945021509346
19	25.562584766935544	24.884114676279534	25.314641480476833	24.23865907630809
20	25.557695760943393	24.25509513256323	25.179150805662783	25.008058300830587
21	26.97506443852325	24.248849252334676	24.183267509934854	24.59281879920722
22	25.701669462488404	24.55318902134342	24.879318652294955	24.865822863873223
23	24.11210429219303	24.285768461159233	25.457583335378192	26.144543911269547
24	24.813777871970196	25.0897368685235	25.21589273962365	24.880592519882654
25	24.947852179053864	24.509364298542092	25.276244018338183	25.266539504065854
26	24.450653950193814	25.468752614406426	25.719958727237234	24.360634708162525
27	25.323318770278874	24.75403235251143	24.966622460713772	24.95602641649592
28	24.39894623195389	24.720608704962793	25.672546710209325	25.207898352873993
29	24.36922019878445	24.875271736402585	25.67478185799103	25.080726206821936
30	24.381503663800313	25.149172996062948	25.878810185030282	24.590513155106457
31	25.109525360362973	24.884674926053652	24.687707730125943	25.318091983457432
32	24.579794910917638	25.108132404476535	24.680621825457905	25.631450859147925
33	24.1537692791591	24.580398053011272	25.581665112585384	25.68416755524424
34	25.19003762270881	24.790833131630404	25.60285009029227	24.416279155368514
35	25.75877569686897	24.66029380598097	25.62860425427491	23.95232624287515
36	24.38796134351799	25.446954418903484	25.03837093089663	25.126713306681896
37	25.343563558003435	25.252548912006905	24.8863711144498	24.51751641553986
38	24.56594114399478	25.107211171153082	25.30127872673838	25.02556895811375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	203.0
1	171.5
2	140.0
3	140.0
4	326.5
5	513.0
6	513.0
7	600.0
8	687.0
9	679.5
10	672.0
11	672.0
12	874.0
13	1076.0
14	1410.5
15	1745.0
16	1745.0
17	2593.0
18	3441.0
19	3441.0
20	4184.5
21	4928.0
22	5665.5
23	6403.0
24	6403.0
25	7857.0
26	9311.0
27	9311.0
28	11909.5
29	14508.0
30	17841.5
31	21175.0
32	21175.0
33	27202.5
34	33230.0
35	33230.0
36	36374.0
37	39518.0
38	45020.0
39	50522.0
40	50522.0
41	53226.5
42	55931.0
43	62904.5
44	69878.0
45	69878.0
46	72071.0
47	74264.0
48	74264.0
49	78859.0
50	83454.0
51	83615.0
52	83776.0
53	83776.0
54	79709.0
55	75642.0
56	75642.0
57	73348.5
58	71055.0
59	63637.5
60	56220.0
61	56220.0
62	53511.5
63	50803.0
64	41655.0
65	32507.0
66	32507.0
67	27140.5
68	21774.0
69	21774.0
70	17391.0
71	13008.0
72	10093.0
73	7178.0
74	7178.0
75	5360.0
76	3542.0
77	3542.0
78	3485.5
79	3429.0
80	2696.5
81	1964.0
82	1964.0
83	1999.0
84	2034.0
85	2034.0
86	1330.5
87	627.0
88	569.0
89	511.0
90	511.0
91	378.0
92	245.0
93	200.0
94	155.0
95	155.0
96	154.0
97	153.0
98	153.0
99	289.5
100	426.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12334829275256287
2	0.05855140478760896
3	0.012602492839999643
4	0.002788162132743284
5	5.576324265486567E-4
6	1.1152648530973136E-4
7	0.0
8	5.576324265486567E-4
9	0.0
10	4.461059412389254E-4
11	0.002565109162123821
12	0.0026766356474335526
13	0.014498443090265076
14	0.010260436648495285
15	0.023755141370972777
16	0.011933333928141256
17	0.021078505723539226
18	0.006803115603893612
19	0.008029906942300657
20	0.006357009662654687
21	0.006357009662654687
22	0.008029906942300657
23	0.010037383677875822
24	0.015390654972742928
25	0.017621184678937554
26	0.019294081958583523
27	0.009702804221946628
28	0.006245483177344956
29	0.011041122045663404
30	0.004238006441769792
31	0.00579937723610603
32	0.006914642089203345
33	0.009368224766017433
34	0.012825545810619106
35	0.015390654972742928
36	0.015167602002123465
37	0.00992585719256609
38	0.005910903721415762
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	896648.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.10411089592247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.81007594239797	50.3733009863757
2	10.42211347197844	12.528237277793593
3	2.6366996040578328	4.754294562045805
4	1.04043232285266	2.5013703884975387
5	0.5269340257048626	1.583545055789996
6	0.3355600593896732	1.2101123413079549
7	0.21564994605741022	0.9073013790773999
8	0.14121525542174698	0.6790093897651758
9	0.1160987322809162	0.6280209971899381
>10	0.5865843260779486	6.667233028540124
>50	0.07573920293793467	3.201220580674603
>100	0.0833835513264049	10.451456614412033
>500	0.00802123645473323	3.4104186612156506
>1k	0.0014923230613457172	1.104478737314596
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1833	0.20442804757273758	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1278	0.14253084822583667	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1185	0.13215888509203164	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1171	0.1305975142976954	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1156	0.12892461701804944	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1088	0.12134081601698772	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1084	0.12089471007574878	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1056	0.11777196848707633	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	997	0.11119190585380218	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	995	0.1109688528831827	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	942	0.10505794916176693	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.1152648530973136E-4	0.0
9	0.0	0.0	0.0	2.230529706194627E-4	0.0
10	0.0	0.0	0.0	2.230529706194627E-4	0.0
11	0.0	0.0	0.0	2.230529706194627E-4	0.0
12	0.0	0.0	0.0	2.230529706194627E-4	1.1152648530973136E-4
13	0.0	0.0	0.0	2.230529706194627E-4	1.1152648530973136E-4
14	0.0	0.0	0.0	2.230529706194627E-4	1.1152648530973136E-4
15	0.0	0.0	0.0	2.230529706194627E-4	1.1152648530973136E-4
16	0.0	0.0	0.0	2.230529706194627E-4	1.1152648530973136E-4
17	0.0	0.0	0.0	3.345794559291941E-4	1.1152648530973136E-4
18	0.0	0.0	0.0	4.461059412389254E-4	1.1152648530973136E-4
19	0.0	0.0	0.0	5.576324265486568E-4	1.1152648530973136E-4
20	0.0	0.0	0.0	7.806853971681195E-4	2.230529706194627E-4
21	0.0	0.0	0.0	8.922118824778508E-4	3.345794559291941E-4
22	0.0	0.0	0.0	0.0013383178237167763	3.345794559291941E-4
23	0.0	0.0	0.0	0.0018959502502654331	3.345794559291941E-4
24	0.0	0.0	0.0	0.0022305297061946273	3.345794559291941E-4
25	0.0	0.0	0.0	0.0026766356474335526	3.345794559291941E-4
26	0.0	0.0	0.0	0.003457321044601672	3.345794559291941E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGT	40	0.0044889227	19.995226	6
GCTCTAA	65	9.340794E-6	19.713993	1
GTCCTAC	280	0.0	19.449966	1
GGTCTAT	60	1.12826296E-4	18.687222	1
GTATTAC	70	1.7696957E-5	18.305851	1
CTAGCGG	195	0.0	18.049992	29
CGCGGTC	135	2.3646862E-11	17.777498	10
AACCGCG	135	2.3646862E-11	17.775517	7
CGTAAAC	55	0.0013649815	17.453299	32
TCCTACA	355	0.0	17.130316	2
TAGCGGC	200	0.0	16.7988	30
TCTAGCG	210	0.0	16.760706	28
TAGGACG	250	0.0	16.636026	4
CGGTCCT	135	4.4201443E-10	16.592333	12
GAACCGC	155	1.0913936E-11	16.512186	6
CGACCCG	165	1.8189894E-12	16.480913	5
GTCGTAA	60	0.002442508	15.998857	30
CTACAGT	485	0.0	15.501453	4
ATAGTAT	125	5.1470124E-8	15.356333	6
AGGACGT	265	0.0	15.090735	5
>>END_MODULE