FastQCFastQC Report
Thu 2 Feb 2017
SRR4063094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896648
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT20300.22639876517875465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15470.1725314727741544No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12390.13818131529875716No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12150.1355046796513236No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12090.1348355207394652No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11310.12613645488530617No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11280.12580187542937696No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11060.12334829275256287No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10620.11844112739893471No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10590.11810654794300551No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10020.11174953828035081No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9670.10784611129451022No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9350.10427726376459881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG2250.022.04325528
CTAGCGG2300.020.86843929
GTATTAG2050.017.9532531
TAGCGGC2800.017.14193230
ATTACAC957.68312E-716.8430753
ACCGTCG2100.016.7638038
AGCGGCG3000.016.5324431
ATTAGCT1551.0913936E-1116.5170783
AGGGGCG2050.016.38935932
TACCGTC2250.016.3574097
TAGGACG2750.016.2927554
TATACTG1304.78758E-916.0018145
ATACCGT2450.015.6752456
CATCGTT2350.015.65872828
AGCCTAT756.237092E-414.9350256
AGGACGT2900.014.8982395
CGTTTAT2150.014.88291631
CGAAAAC2700.014.8148423
AAATTCG650.00416058114.76925721
TCTAGAT2400.014.6666932
Produced by FastQC (version 0.11.5)