##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063094_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	896648
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.246099918808717	32.0	32.0	32.0	32.0	32.0
2	31.30756216486291	32.0	32.0	32.0	32.0	32.0
3	31.38786569534533	32.0	32.0	32.0	32.0	32.0
4	31.48691459747861	32.0	32.0	32.0	32.0	32.0
5	31.410700743212498	32.0	32.0	32.0	32.0	32.0
6	34.94034336774297	36.0	36.0	36.0	36.0	36.0
7	34.970391948679975	36.0	36.0	36.0	36.0	36.0
8	34.91013084287257	36.0	36.0	36.0	36.0	36.0
9	35.00827303468028	36.0	36.0	36.0	36.0	36.0
10	34.858279949322366	36.0	36.0	36.0	32.0	36.0
11	35.03772829471543	36.0	36.0	36.0	36.0	36.0
12	34.93652804668053	36.0	36.0	36.0	32.0	36.0
13	34.99150948867337	36.0	36.0	36.0	36.0	36.0
14	34.94499402218039	36.0	36.0	36.0	32.0	36.0
15	34.91385694274676	36.0	36.0	36.0	32.0	36.0
16	34.911171384980506	36.0	36.0	36.0	32.0	36.0
17	34.8864682684844	36.0	36.0	36.0	32.0	36.0
18	34.88539203790116	36.0	36.0	36.0	32.0	36.0
19	34.879733184036546	36.0	36.0	36.0	32.0	36.0
20	34.86481428609666	36.0	36.0	36.0	32.0	36.0
21	34.853919263746754	36.0	36.0	36.0	32.0	36.0
22	34.83656128157315	36.0	36.0	36.0	32.0	36.0
23	34.77783924126302	36.0	36.0	36.0	32.0	36.0
24	34.75458262328138	36.0	36.0	36.0	32.0	36.0
25	34.73517255377807	36.0	36.0	36.0	32.0	36.0
26	34.66604174659398	36.0	36.0	36.0	32.0	36.0
27	34.64685584532615	36.0	36.0	36.0	32.0	36.0
28	34.62615429912296	36.0	36.0	36.0	32.0	36.0
29	34.583228870192094	36.0	36.0	36.0	32.0	36.0
30	34.55791124276193	36.0	36.0	36.0	32.0	36.0
31	34.55730899974126	36.0	36.0	36.0	32.0	36.0
32	34.52871026311328	36.0	36.0	36.0	32.0	36.0
33	34.49257791240264	36.0	36.0	36.0	32.0	36.0
34	34.4972988285258	36.0	36.0	36.0	32.0	36.0
35	34.44740745532249	36.0	36.0	36.0	32.0	36.0
36	34.43261904337042	36.0	36.0	36.0	32.0	36.0
37	34.41807822021574	36.0	36.0	36.0	32.0	36.0
38	33.972625824180724	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	14.0
22	55.0
23	190.0
24	550.0
25	1366.0
26	2870.0
27	5641.0
28	9892.0
29	16303.0
30	24496.0
31	35847.0
32	51123.0
33	82163.0
34	203278.0
35	462857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.95341462843351	18.346608013437894	11.856644608501966	26.84333274962663
2	15.792931005255129	20.704334365325078	36.79258750368037	26.710147125739418
3	18.035282518892586	24.495342653973466	28.93119708068272	28.538177746451225
4	11.961019175906657	16.067098944287935	36.454632039846274	35.517249839959135
5	13.902110973313942	37.13932334650833	33.69192815909922	15.266637521078504
6	34.10305281788709	36.168414102762846	16.538169424533848	13.19036365481622
7	29.856062954891694	30.627608343129143	21.11322021497989	18.40310848699927
8	27.82980621776105	33.49047818207166	19.441014917050047	19.238700683117248
9	27.39093969088147	13.789099880216012	18.457552525389968	40.36240790351255
10	15.576537662398495	26.857803102035717	31.878787067676218	25.686872167889568
11	37.90026756069189	20.79701634696566	22.21467535263027	19.088040739712188
12	24.60999188087187	23.778004300461273	28.89015533408874	22.721848484578118
13	29.800913849039812	19.19830211889406	25.144231787983458	25.856552244082675
14	23.412085902160047	19.92732934217218	24.924273516474692	31.736311239193082
15	25.19450219038017	27.45703999785869	22.099530696549817	25.248927115211323
16	25.741762653795025	25.536553920825117	23.95455072670658	24.76713269867328
17	24.051913348381973	25.905148954773782	25.32721870789875	24.715718988945497
18	25.154910288095216	24.42775760387577	26.494231850179776	23.923100257849235
19	25.628228691749715	24.891261676823014	25.258629919433268	24.221879711994003
20	25.739755177059447	24.19410961715188	25.19439066389486	24.871744541893808
21	26.948955386010326	24.24030861643434	24.187556530050287	24.62317946750505
22	25.629009377146883	24.632966336845673	24.95405108805239	24.78397319795505
23	24.16173625013244	24.165416636461476	25.572885590172252	26.099961523233834
24	24.715830515430802	25.171527734406368	25.31472774154406	24.797914008618765
25	24.938995012535578	24.45965417867435	25.279596898671496	25.321753910118577
26	24.433556981111877	25.4722031388014	25.756595676341217	24.337644203745505
27	25.386885377539457	24.64478814428851	24.921819933797877	25.046506544374157
28	24.355599967880373	24.58545605410373	25.75971841792989	25.299225560086008
29	24.374559470383026	24.718284098107617	25.711650502761398	25.19550592874796
30	24.428984395214176	24.984497818541946	25.956228085045634	24.63028970119824
31	25.12435203112035	24.711815561959654	24.706685343635407	25.45714706328459
32	24.627501539065495	24.99230467251363	24.724641107770275	25.6555526806506
33	24.31110090024179	24.494785021546917	25.558636164916443	25.635477913294853
34	25.251157644917516	24.636535184375585	25.643953926178387	24.468353244528508
35	25.78313897984493	24.57341119369028	25.517036785895915	24.126413040568874
36	24.527127702286737	25.26119502859539	25.046283491403535	25.16539377771433
37	25.441087249399985	25.026766356474333	24.932861055843542	24.599285338282133
38	24.63742739625806	24.74650029889098	25.453355162784057	25.1627171420669
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102.0
1	99.0
2	96.0
3	96.0
4	308.0
5	520.0
6	520.0
7	609.5
8	699.0
9	692.0
10	685.0
11	685.0
12	848.5
13	1012.0
14	1390.5
15	1769.0
16	1769.0
17	2717.5
18	3666.0
19	3666.0
20	4410.5
21	5155.0
22	5758.0
23	6361.0
24	6361.0
25	7785.0
26	9209.0
27	9209.0
28	11799.5
29	14390.0
30	17683.5
31	20977.0
32	20977.0
33	27127.5
34	33278.0
35	33278.0
36	36631.0
37	39984.0
38	45134.0
39	50284.0
40	50284.0
41	53355.5
42	56427.0
43	63120.0
44	69813.0
45	69813.0
46	72182.0
47	74551.0
48	74551.0
49	78883.0
50	83215.0
51	83560.0
52	83905.0
53	83905.0
54	80208.5
55	76512.0
56	76512.0
57	74100.0
58	71688.0
59	63957.5
60	56227.0
61	56227.0
62	53351.0
63	50475.0
64	41526.5
65	32578.0
66	32578.0
67	27166.0
68	21754.0
69	21754.0
70	17365.5
71	12977.0
72	10016.5
73	7056.0
74	7056.0
75	5235.0
76	3414.0
77	3414.0
78	3396.0
79	3378.0
80	2638.0
81	1898.0
82	1898.0
83	1820.0
84	1742.0
85	1742.0
86	1099.0
87	456.0
88	372.5
89	289.0
90	289.0
91	175.0
92	61.0
93	43.5
94	26.0
95	26.0
96	17.5
97	9.0
98	9.0
99	9.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008810592339468777
2	0.0
3	0.0
4	2.230529706194627E-4
5	0.0
6	1.1152648530973136E-4
7	6.691589118583882E-4
8	0.002565109162123821
9	0.0037919005005308663
10	0.0012267913384070449
11	0.0032342680739822095
12	0.0
13	1.1152648530973136E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.1152648530973136E-4
22	0.0
23	3.345794559291941E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	896648.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.306684344616656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.62161994718585	48.17392714301449
2	11.266097185289013	13.137775447568414
3	2.7434813961486384	4.798899113117006
4	1.1330630014357974	2.6426058706912388
5	0.5820551578726668	1.6968853180618804
6	0.35224042983136866	1.2322782933353824
7	0.22110796312687664	0.9024450548483955
8	0.16716495119322436	0.7797467234165266
9	0.11472831999077576	0.6020485145181302
>10	0.6199994900623661	6.854881057053736
>50	0.07776929613396309	3.200480464412875
>100	0.08950837613915594	10.838777372092276
>500	0.00904708315076191	3.598484856273377
>1k	0.0021174024395400216	1.5407647715963109
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2030	0.22639876517875465	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1547	0.1725314727741544	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1239	0.13818131529875716	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1215	0.1355046796513236	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1209	0.1348355207394652	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1131	0.12613645488530617	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1128	0.12580187542937696	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1106	0.12334829275256287	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1062	0.11844112739893471	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1059	0.11810654794300551	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1002	0.11174953828035081	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	967	0.10784611129451022	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	935	0.10427726376459881	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	1.1152648530973136E-4	0.0	0.0
10	0.0	0.0	1.1152648530973136E-4	0.0	0.0
11	0.0	0.0	1.1152648530973136E-4	0.0	0.0
12	0.0	0.0	1.1152648530973136E-4	0.0	0.0
13	0.0	0.0	1.1152648530973136E-4	0.0	0.0
14	0.0	0.0	1.1152648530973136E-4	0.0	0.0
15	0.0	0.0	1.1152648530973136E-4	0.0	0.0
16	0.0	0.0	1.1152648530973136E-4	0.0	0.0
17	0.0	0.0	1.1152648530973136E-4	1.1152648530973136E-4	0.0
18	0.0	0.0	1.1152648530973136E-4	2.230529706194627E-4	0.0
19	0.0	0.0	1.1152648530973136E-4	3.345794559291941E-4	0.0
20	0.0	0.0	1.1152648530973136E-4	5.576324265486568E-4	1.1152648530973136E-4
21	0.0	0.0	1.1152648530973136E-4	6.691589118583882E-4	1.1152648530973136E-4
22	0.0	0.0	1.1152648530973136E-4	8.922118824778508E-4	1.1152648530973136E-4
23	0.0	0.0	1.1152648530973136E-4	0.001561370794336239	1.1152648530973136E-4
24	0.0	0.0	1.1152648530973136E-4	0.0020074767355751644	1.1152648530973136E-4
25	0.0	0.0	1.1152648530973136E-4	0.0024535826768140897	1.1152648530973136E-4
26	0.0	0.0	1.1152648530973136E-4	0.003122741588672478	1.1152648530973136E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	225	0.0	22.043255	28
CTAGCGG	230	0.0	20.868439	29
GTATTAG	205	0.0	17.953253	1
TAGCGGC	280	0.0	17.141932	30
ATTACAC	95	7.68312E-7	16.843075	3
ACCGTCG	210	0.0	16.763803	8
AGCGGCG	300	0.0	16.53244	31
ATTAGCT	155	1.0913936E-11	16.517078	3
AGGGGCG	205	0.0	16.389359	32
TACCGTC	225	0.0	16.357409	7
TAGGACG	275	0.0	16.292755	4
TATACTG	130	4.78758E-9	16.001814	5
ATACCGT	245	0.0	15.675245	6
CATCGTT	235	0.0	15.658728	28
AGCCTAT	75	6.237092E-4	14.935025	6
AGGACGT	290	0.0	14.898239	5
CGTTTAT	215	0.0	14.882916	31
CGAAAAC	270	0.0	14.81484	23
AAATTCG	65	0.004160581	14.769257	21
TCTAGAT	240	0.0	14.666693	2
>>END_MODULE