FastQCFastQC Report
Thu 2 Feb 2017
SRR4063093_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063093_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403902
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23650.16845905198511008No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20910.148942020169499No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18930.13483847163121074No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17140.12208829391225315No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16090.11460913938437299No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15690.11175993765946625No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15330.10919565610705022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15110.1076285951583515No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14850.10577661403716214No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14760.10513554364905812No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14230.10136035136355671No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14220.10128912132043404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16400.020.2115731
AAGACGG4800.018.6612455
ACCGTCG3350.018.1465688
CAAGACG4850.018.1397044
GCGCAAG4900.017.9738121
CTAGCGG3850.017.45569229
GGTATCA6550.017.3576371
TACCGTC3600.016.8863877
ATACCGT3950.016.602776
TCTAGCG4300.016.37258528
CGCAAGA5250.016.157472
GTCTATA703.6742754E-416.0130331
TAGCGGC4200.015.99991130
GACGGAC5600.015.711967
CCGTCGT3950.015.3928679
CGTCGTA4100.015.2199710
TAGAACT2000.015.1961254
ACGGACC5900.015.1841928
CTATTCC4350.015.0766164
AGCGAAA5950.015.05927817
Produced by FastQC (version 0.11.5)