##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063093_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1403902
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.254319033664743	32.0	32.0	32.0	32.0	32.0
2	30.871701870928312	32.0	32.0	32.0	32.0	32.0
3	30.917990002151146	32.0	32.0	32.0	32.0	32.0
4	30.994069386609606	32.0	32.0	32.0	32.0	32.0
5	30.89754626747451	32.0	32.0	32.0	32.0	32.0
6	34.55070012009385	36.0	36.0	36.0	32.0	36.0
7	34.49047939243622	36.0	36.0	36.0	32.0	36.0
8	34.476207740996166	36.0	36.0	36.0	32.0	36.0
9	34.627572294932264	36.0	36.0	36.0	32.0	36.0
10	34.34086424835922	36.0	36.0	36.0	32.0	36.0
11	34.58215601943726	36.0	36.0	36.0	32.0	36.0
12	34.413401362773186	36.0	36.0	36.0	32.0	36.0
13	34.48926064639839	36.0	36.0	36.0	32.0	36.0
14	34.40452111329708	36.0	36.0	36.0	32.0	36.0
15	34.343545347182356	36.0	36.0	36.0	32.0	36.0
16	34.360282270414885	36.0	36.0	36.0	32.0	36.0
17	34.28350839303598	36.0	36.0	36.0	32.0	36.0
18	34.305999991452396	36.0	36.0	36.0	32.0	36.0
19	34.30284450054206	36.0	36.0	36.0	32.0	36.0
20	34.29393932055086	36.0	36.0	36.0	32.0	36.0
21	34.28569159385769	36.0	36.0	36.0	32.0	36.0
22	34.25143421691828	36.0	36.0	36.0	32.0	36.0
23	34.192233503478164	36.0	36.0	36.0	32.0	36.0
24	34.17577437741381	36.0	36.0	36.0	32.0	36.0
25	34.15714202273378	36.0	36.0	36.0	32.0	36.0
26	34.104992371262384	36.0	36.0	36.0	32.0	36.0
27	34.11169796752195	36.0	36.0	36.0	32.0	36.0
28	34.1062930318498	36.0	36.0	36.0	32.0	36.0
29	34.0730578060292	36.0	36.0	36.0	32.0	36.0
30	34.04911240243265	36.0	36.0	36.0	32.0	36.0
31	34.07017797538575	36.0	36.0	36.0	32.0	36.0
32	34.02286341924152	36.0	36.0	36.0	32.0	36.0
33	33.988100308995925	36.0	36.0	36.0	32.0	36.0
34	34.001175295711526	36.0	36.0	36.0	32.0	36.0
35	33.96492846366769	36.0	36.0	36.0	32.0	36.0
36	33.92983840752417	36.0	36.0	36.0	32.0	36.0
37	33.9403890015115	36.0	36.0	36.0	32.0	36.0
38	33.36316281335877	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	2.0
9	10.0
10	8.0
11	14.0
12	7.0
13	5.0
14	90.0
15	245.0
16	358.0
17	375.0
18	534.0
19	711.0
20	991.0
21	1460.0
22	2237.0
23	3688.0
24	5756.0
25	8819.0
26	12931.0
27	19125.0
28	27148.0
29	36572.0
30	50213.0
31	67294.0
32	91957.0
33	137052.0
34	302252.0
35	634046.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.496163389197584	16.95247794635235	11.26998628533772	26.28137237911235
2	17.24012592180276	19.05504481883952	36.83084442727603	26.873984832081693
3	19.15417905928868	22.50939464638729	28.445030187536734	29.891396106787298
4	12.960638693436552	14.591563582279221	35.1923189250256	37.25547879925862
5	15.139254932687512	35.773274449747134	33.40223662654035	15.685233991025003
6	35.56500704469141	34.660878955248755	16.074646661357626	13.69946733870221
7	31.025834442742045	29.930885439927746	20.303069803355083	18.74021031397513
8	28.58717044459751	32.68967354489176	18.977576022206726	19.745579988304
9	27.422841671658716	13.95855399854263	18.35466796400596	40.263936365792695
10	16.26865522802259	26.016955671930113	31.034065201410932	26.680323898636367
11	38.40130951923036	20.780924783848125	21.3842552821692	19.433510414752323
12	24.78062318046704	23.422014826233045	27.905571982451278	23.891790010848638
13	29.500769039516307	18.64651894743816	25.041265969413672	26.81144604363186
14	23.75930176396921	19.11557909224187	24.449089093196445	32.67603005059247
15	25.55688946995308	26.593329082223356	21.630502545322415	26.21927890250115
16	26.48924862936409	25.362035949984612	22.829992705139457	25.31872271551184
17	24.756410000655464	25.60095982581783	24.351305368811932	25.291324804714776
18	25.71720223988352	24.262175502182252	25.24242728606099	24.778194971873233
19	25.854843477858235	24.550664448868957	24.836959812508237	24.75753226076457
20	26.06146389555326	23.865022196641156	24.391943103654608	25.681570804150972
21	27.326644935547478	23.794507003726963	23.99324986608008	24.885598194645482
22	26.351298406180945	23.66594054130295	24.494193609052015	25.48856744346409
23	24.78584439462618	23.50023401286109	25.262062347864926	26.451859244647803
24	25.124920744608854	24.499497752352728	24.638987240772536	25.736594262265882
25	25.304335250026185	23.900976964776405	24.73787663869402	26.056811146503396
26	25.28132433855385	24.856781955915704	24.993498994392393	24.868394711138055
27	26.013477895396715	24.047357848095853	24.276453575346565	25.662710681160867
28	25.04852833591795	24.058799603082733	25.35654525262872	25.536126808370597
29	25.12172794916456	24.36254447923234	25.171808270020552	25.343919301582552
30	24.949442928385256	24.535797398456683	25.301330119335347	25.213429553822714
31	25.55213159409388	24.359609981821187	24.239652608004377	25.848605816080557
32	25.476277315066774	24.108636248365155	24.085698573305105	26.329387863262966
33	24.881355207761953	23.928492559322397	24.818595638886713	26.371556594028938
34	25.847030193883715	24.09526631940091	24.70891796701355	25.34878551970182
35	26.28653173059044	23.804479235607623	25.04472182703001	24.864267206771927
36	24.9306818117053	24.652482916236842	24.49703349500453	25.91980177705333
37	26.191398875884225	24.306973364582518	24.406134908140224	25.095492851393036
38	25.119904375681617	24.004382306366807	24.942888261872724	25.932825056078844
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	34.5
2	56.0
3	56.0
4	200.0
5	344.0
6	344.0
7	426.0
8	508.0
9	490.0
10	472.0
11	472.0
12	808.5
13	1145.0
14	1646.0
15	2147.0
16	2147.0
17	3109.5
18	4072.0
19	4072.0
20	5209.5
21	6347.0
22	7071.0
23	7795.0
24	7795.0
25	9379.0
26	10963.0
27	10963.0
28	14089.0
29	17215.0
30	21052.5
31	24890.0
32	24890.0
33	32836.5
34	40783.0
35	40783.0
36	46772.0
37	52761.0
38	61613.5
39	70466.0
40	70466.0
41	76443.5
42	82421.0
43	94752.0
44	107083.0
45	107083.0
46	111280.5
47	115478.0
48	115478.0
49	124971.5
50	134465.0
51	137982.0
52	141499.0
53	141499.0
54	134518.0
55	127537.0
56	127537.0
57	124176.0
58	120815.0
59	108425.0
60	96035.0
61	96035.0
62	90905.5
63	85776.0
64	70809.0
65	55842.0
66	55842.0
67	47335.0
68	38828.0
69	38828.0
70	30822.5
71	22817.0
72	17714.0
73	12611.0
74	12611.0
75	9798.5
76	6986.0
77	6986.0
78	6480.5
79	5975.0
80	4724.0
81	3473.0
82	3473.0
83	3343.0
84	3213.0
85	3213.0
86	2125.0
87	1037.0
88	884.0
89	731.0
90	731.0
91	524.0
92	317.0
93	285.5
94	254.0
95	254.0
96	220.0
97	186.0
98	186.0
99	366.0
100	546.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12486626559403718
2	0.0576251048862385
3	0.012607717632712255
4	0.0025642815524160516
5	1.4246008624533622E-4
6	5.698403449813449E-4
7	7.123004312266811E-5
8	2.1369012936800431E-4
9	2.1369012936800431E-4
10	4.2738025873600863E-4
11	0.001994441207434707
12	0.002777971681784056
13	0.014459698753901625
14	0.009687285864682862
15	0.02471682496356583
16	0.01239402750334425
17	0.022366233540517786
18	0.00562717340669078
19	0.007621614614125488
20	0.006125783708549458
21	0.005555943363568112
22	0.007407924484757482
23	0.009473595735314859
24	0.01510076914200564
25	0.01916088159999772
26	0.01944580177248839
27	0.008690065260965508
28	0.005912093579181453
29	0.011183116770258893
30	0.003062891854274729
31	0.004986103018586768
32	0.006980544226021474
33	0.00940236569219219
34	0.01239402750334425
35	0.016026759702600327
36	0.016382909918213666
37	0.00940236569219219
38	0.005342253234200108
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1403902.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.69848303251859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.25453997499883	48.03660662127611
2	9.94931723704051	11.481210235730533
3	2.8426062672617176	4.9204220843919355
4	1.2344657351853827	2.8490720110327756
5	0.6769417106338381	1.9529254902505306
6	0.4403116637734159	1.524318903675028
7	0.27952244034640894	1.1289614547074827
8	0.2007683962976498	0.9267225525796733
9	0.16130410100873452	0.8376301741615318
>10	0.8055130312255712	8.311531921945885
>50	0.07010189923844656	2.842378024396985
>100	0.07220178819266064	8.924385526260735
>500	0.009056201001670927	3.5531260275478522
>1k	0.0033495537951385625	2.7107089720428927
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2365	0.16845905198511008	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2091	0.148942020169499	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1893	0.13483847163121074	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1714	0.12208829391225315	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1609	0.11460913938437299	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1569	0.11175993765946625	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1533	0.10919565610705022	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1511	0.1076285951583515	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1485	0.10577661403716214	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1476	0.10513554364905812	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1423	0.10136035136355671	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1422	0.10128912132043404	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.123004312266811E-5
2	0.0	0.0	0.0	0.0	7.123004312266811E-5
3	0.0	0.0	0.0	0.0	7.123004312266811E-5
4	0.0	0.0	0.0	0.0	7.123004312266811E-5
5	0.0	0.0	0.0	0.0	7.123004312266811E-5
6	0.0	0.0	0.0	0.0	7.123004312266811E-5
7	0.0	0.0	0.0	0.0	7.123004312266811E-5
8	0.0	0.0	0.0	0.0	7.123004312266811E-5
9	0.0	0.0	0.0	0.0	7.123004312266811E-5
10	0.0	0.0	0.0	0.0	7.123004312266811E-5
11	0.0	0.0	0.0	0.0	7.123004312266811E-5
12	0.0	0.0	0.0	0.0	7.123004312266811E-5
13	0.0	0.0	0.0	0.0	7.123004312266811E-5
14	0.0	0.0	0.0	7.123004312266811E-5	7.123004312266811E-5
15	0.0	0.0	0.0	7.123004312266811E-5	2.1369012936800431E-4
16	0.0	0.0	0.0	7.123004312266811E-5	3.561502156133405E-4
17	0.0	0.0	0.0	7.123004312266811E-5	3.561502156133405E-4
18	0.0	0.0	0.0	7.123004312266811E-5	4.2738025873600863E-4
19	0.0	0.0	0.0	7.123004312266811E-5	4.2738025873600863E-4
20	0.0	0.0	0.0	7.123004312266811E-5	4.2738025873600863E-4
21	0.0	0.0	0.0	7.123004312266811E-5	4.2738025873600863E-4
22	0.0	0.0	0.0	7.123004312266811E-5	4.2738025873600863E-4
23	0.0	0.0	0.0	7.123004312266811E-5	4.2738025873600863E-4
24	0.0	0.0	0.0	2.8492017249067244E-4	4.2738025873600863E-4
25	0.0	0.0	0.0	3.561502156133405E-4	4.2738025873600863E-4
26	0.0	0.0	0.0	4.986103018586768E-4	4.2738025873600863E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1640	0.0	20.211573	1
AAGACGG	480	0.0	18.661245	5
ACCGTCG	335	0.0	18.146568	8
CAAGACG	485	0.0	18.139704	4
GCGCAAG	490	0.0	17.973812	1
CTAGCGG	385	0.0	17.455692	29
GGTATCA	655	0.0	17.357637	1
TACCGTC	360	0.0	16.886387	7
ATACCGT	395	0.0	16.60277	6
TCTAGCG	430	0.0	16.372585	28
CGCAAGA	525	0.0	16.15747	2
GTCTATA	70	3.6742754E-4	16.013033	1
TAGCGGC	420	0.0	15.999911	30
GACGGAC	560	0.0	15.71196	7
CCGTCGT	395	0.0	15.392867	9
CGTCGTA	410	0.0	15.21997	10
TAGAACT	200	0.0	15.196125	4
ACGGACC	590	0.0	15.184192	8
CTATTCC	435	0.0	15.076616	4
AGCGAAA	595	0.0	15.059278	17
>>END_MODULE