FastQCFastQC Report
Thu 2 Feb 2017
SRR4063093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403902
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20570.1465201987033283No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19520.13904104417544813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT17480.12451011537842385No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16980.12094861322229043No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16820.11980893253232774No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16010.11403929903939164No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16000.11396806899626898No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15330.10919565610705022No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15320.10912442606392754No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14870.10591907412340748No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14650.10435201317470877No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14270.1016452715360474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCG356.427604E-527.4311058
GTATCGA400.004481569420.0018489
GAACGGT659.428728E-619.6941266
GTATCAA16800.019.334431
TGCGTAG802.798417E-617.999113
CTAGCGG4400.017.81729129
TCTAGCG4450.017.61709428
GTATTAC854.9828213E-616.9421391
TAGCGGC4750.016.50443630
AAGACGG4550.016.1773195
CGGTCCA4950.016.16195710
GGTATCA6300.016.0009081
TAGTACG805.6246907E-515.9991992
CAAGACG4700.015.65990454
GCATTCG4050.015.01266322
GCGTGTT756.247947E-414.93258530
CCGGTCC5800.014.8979289
GTATAGG1401.27802195E-814.85798551
ACCGTCG3600.014.6680228
TGTGCGT1205.417387E-714.66697711
Produced by FastQC (version 0.11.5)