##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063093_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1403902
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22720033164708	32.0	32.0	32.0	32.0	32.0
2	31.245146028711407	32.0	32.0	32.0	32.0	32.0
3	31.33900087043113	32.0	32.0	32.0	32.0	32.0
4	31.44443415566044	32.0	32.0	32.0	32.0	32.0
5	31.36386015548094	32.0	32.0	32.0	32.0	32.0
6	34.88618436329601	36.0	36.0	36.0	36.0	36.0
7	34.91185424623656	36.0	36.0	36.0	36.0	36.0
8	34.84147611442964	36.0	36.0	36.0	32.0	36.0
9	34.96513787999447	36.0	36.0	36.0	32.0	36.0
10	34.799951136190415	36.0	36.0	36.0	32.0	36.0
11	34.97630390155438	36.0	36.0	36.0	36.0	36.0
12	34.86618083028588	36.0	36.0	36.0	32.0	36.0
13	34.9346749274522	36.0	36.0	36.0	36.0	36.0
14	34.88064195364064	36.0	36.0	36.0	32.0	36.0
15	34.840528042555675	36.0	36.0	36.0	32.0	36.0
16	34.85117835860338	36.0	36.0	36.0	32.0	36.0
17	34.82338083427476	36.0	36.0	36.0	32.0	36.0
18	34.82640811110747	36.0	36.0	36.0	32.0	36.0
19	34.821989711532574	36.0	36.0	36.0	32.0	36.0
20	34.8116777381897	36.0	36.0	36.0	32.0	36.0
21	34.8106762437834	36.0	36.0	36.0	32.0	36.0
22	34.792853774693675	36.0	36.0	36.0	32.0	36.0
23	34.72748667642043	36.0	36.0	36.0	32.0	36.0
24	34.703735730841615	36.0	36.0	36.0	32.0	36.0
25	34.68554072862636	36.0	36.0	36.0	32.0	36.0
26	34.627575856434426	36.0	36.0	36.0	32.0	36.0
27	34.61554367755014	36.0	36.0	36.0	32.0	36.0
28	34.58419889707401	36.0	36.0	36.0	32.0	36.0
29	34.555569405841716	36.0	36.0	36.0	32.0	36.0
30	34.53238402680529	36.0	36.0	36.0	32.0	36.0
31	34.53774195064898	36.0	36.0	36.0	32.0	36.0
32	34.5055773123765	36.0	36.0	36.0	32.0	36.0
33	34.47584019397365	36.0	36.0	36.0	32.0	36.0
34	34.47978562606222	36.0	36.0	36.0	32.0	36.0
35	34.452612789211784	36.0	36.0	36.0	32.0	36.0
36	34.429741534665524	36.0	36.0	36.0	32.0	36.0
37	34.41924080170838	36.0	36.0	36.0	32.0	36.0
38	33.97426387311935	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	25.0
22	83.0
23	286.0
24	784.0
25	2051.0
26	4429.0
27	9083.0
28	16207.0
29	25632.0
30	40059.0
31	58206.0
32	84941.0
33	135500.0
34	327380.0
35	699228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94191397512431	18.14999501346365	11.87728846400433	27.030802547407713
2	16.262032535034496	20.112728666245935	36.03250084407601	27.59273795464356
3	18.182038347406014	23.425424281751862	28.67479353972001	29.717743831122117
4	12.473573541060022	15.493882737752655	35.10302757469214	36.92951614649518
5	14.663559137318702	36.422912710431355	32.96248598548902	15.951042166760928
6	35.607735593703254	35.066386494764586	15.768929410926704	13.556948500605456
7	30.918480370682992	30.095982961688726	20.23000295606153	18.75553371156675
8	28.561242450339098	32.93550475791579	18.7666103224584	19.736642469286707
9	27.34762551964463	13.858519892523672	18.018882439929225	40.774972147902474
10	16.04115692412894	26.20124724427397	31.05481535300005	26.70278047859704
11	38.63175935582036	20.77385328158925	21.263299312168407	19.33108805042199
12	24.619112710156685	23.6183954190241	27.81095140441615	23.951540466403067
13	29.587804224814374	18.655512943978756	25.00790656857773	26.748776262629143
14	23.650297527890125	19.13545247460293	24.382684831277395	32.83156516622955
15	25.53148296675979	26.7331337942392	21.468450076999677	26.266933162001337
16	26.532677920562662	25.326964404134483	22.72884302930624	25.411514645996615
17	24.78855361699036	25.600718568675024	24.297137549487072	25.313590264847548
18	25.744318335610323	24.4131712897339	25.146270893552398	24.696239481103383
19	25.965131469290593	24.676793679330896	24.623727297204507	24.73434755417401
20	26.205461634786474	23.832931358456644	24.153039172249915	25.808567834506967
21	27.312629104637082	23.963743856400026	23.82156848778403	24.90205855117886
22	26.370216724529204	23.797458796981555	24.38432312226922	25.448001356220022
23	24.783905394903766	23.610957768329488	24.981426726566514	26.623710110200232
24	25.202756317748676	24.59523527995544	24.465739061558427	25.73626934073746
25	25.406724125649976	23.988745637153645	24.669492129069024	25.93503810812736
26	25.31341263622765	24.888453593560794	24.851983759527034	24.946150010684523
27	26.09647966880879	24.185092691655115	24.073047833823157	25.64537980571294
28	25.133805636005935	24.127823736984492	25.11208047285352	25.626290154156063
29	25.095911253064674	24.368866202911597	25.059299010899622	25.475923533124107
30	25.057233339649066	24.503847134629055	25.126682631693665	25.312236894028217
31	25.6776470152475	24.31017264737852	24.128464807372595	25.883715530001382
32	25.659697044380593	24.264371729650644	23.902380650501247	26.17355057546752
33	24.92595637017399	24.002815011304207	24.70557061675245	26.365658001769354
34	25.812271796749346	24.106953334349548	24.74018841771007	25.340586451191037
35	26.378693099660804	23.814554007330997	24.839696787952438	24.967056105055764
36	25.05132124606988	24.624510827678854	24.428058368746537	25.896109557504726
37	26.2184255026348	24.374065996059553	24.237375543307156	25.170132957998494
38	25.22008318250361	23.973271619580014	24.845626579082143	25.961018618834235
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	17.5
2	25.0
3	25.0
4	119.0
5	213.0
6	213.0
7	292.5
8	372.0
9	376.0
10	380.0
11	380.0
12	737.5
13	1095.0
14	1519.5
15	1944.0
16	1944.0
17	2730.0
18	3516.0
19	3516.0
20	4661.5
21	5807.0
22	6736.0
23	7665.0
24	7665.0
25	9346.5
26	11028.0
27	11028.0
28	14089.0
29	17150.0
30	21221.5
31	25293.0
32	25293.0
33	33292.5
34	41292.0
35	41292.0
36	47232.0
37	53172.0
38	61897.0
39	70622.0
40	70622.0
41	77069.5
42	83517.0
43	95908.5
44	108300.0
45	108300.0
46	111513.5
47	114727.0
48	114727.0
49	124680.5
50	134634.0
51	137896.5
52	141159.0
53	141159.0
54	134485.0
55	127811.0
56	127811.0
57	124756.5
58	121702.0
59	109083.0
60	96464.0
61	96464.0
62	90923.0
63	85382.0
64	70825.0
65	56268.0
66	56268.0
67	47513.0
68	38758.0
69	38758.0
70	30962.0
71	23166.0
72	17894.5
73	12623.0
74	12623.0
75	9568.5
76	6514.0
77	6514.0
78	6210.5
79	5907.0
80	4583.0
81	3259.0
82	3259.0
83	3034.0
84	2809.0
85	2809.0
86	1762.0
87	715.0
88	587.0
89	459.0
90	459.0
91	274.0
92	89.0
93	58.0
94	27.0
95	27.0
96	20.5
97	14.0
98	14.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008690065260965508
2	0.0
3	0.0
4	4.2738025873600863E-4
5	0.0
6	1.4246008624533622E-4
7	4.986103018586768E-4
8	0.002208131336802711
9	0.00413134250111475
10	4.986103018586768E-4
11	0.004986103018586768
12	6.410703881040129E-4
13	4.2738025873600863E-4
14	0.0
15	0.0
16	2.1369012936800431E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.4246008624533622E-4
22	0.0
23	2.1369012936800431E-4
24	0.0
25	1.4246008624533622E-4
26	1.4246008624533622E-4
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	7.123004312266811E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1403902.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.07507305560683
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.14719396546569	46.06409902926593
2	10.67560513943882	11.972706762136871
3	2.936362621022208	4.939702454747202
4	1.308718475337951	2.9354593645519196
5	0.7500593421166649	2.102981620261619
6	0.4570674915174954	1.5378055786911902
7	0.2956489062959016	1.1604973813547057
8	0.2222121790657015	0.9968451339963837
9	0.16883472067666436	0.8520677366640232
>10	0.8704302588119802	8.618264455256027
>50	0.07940256038227408	3.098870695392162
>100	0.07440212569011677	8.922914199017015
>500	0.009971388235685288	3.7604740837114603
>1k	0.004090825942845247	3.0373115049535735
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2057	0.1465201987033283	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1952	0.13904104417544813	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1748	0.12451011537842385	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1698	0.12094861322229043	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1682	0.11980893253232774	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1601	0.11403929903939164	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1600	0.11396806899626898	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1533	0.10919565610705022	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1532	0.10912442606392754	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1487	0.10591907412340748	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1465	0.10435201317470877	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1427	0.1016452715360474	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	7.123004312266811E-5	0.0	0.0	0.0	0.0
11	7.123004312266811E-5	0.0	0.0	0.0	0.0
12	7.123004312266811E-5	0.0	0.0	0.0	0.0
13	7.123004312266811E-5	0.0	0.0	0.0	0.0
14	7.123004312266811E-5	0.0	0.0	7.123004312266811E-5	0.0
15	7.123004312266811E-5	0.0	0.0	7.123004312266811E-5	7.123004312266811E-5
16	7.123004312266811E-5	0.0	0.0	1.4246008624533622E-4	7.123004312266811E-5
17	7.123004312266811E-5	0.0	0.0	1.4246008624533622E-4	1.4246008624533622E-4
18	7.123004312266811E-5	0.0	0.0	2.1369012936800431E-4	2.1369012936800431E-4
19	7.123004312266811E-5	0.0	0.0	2.1369012936800431E-4	2.1369012936800431E-4
20	7.123004312266811E-5	0.0	0.0	2.1369012936800431E-4	2.8492017249067244E-4
21	7.123004312266811E-5	0.0	0.0	2.1369012936800431E-4	2.8492017249067244E-4
22	7.123004312266811E-5	0.0	0.0	2.1369012936800431E-4	2.8492017249067244E-4
23	7.123004312266811E-5	0.0	0.0	2.1369012936800431E-4	2.8492017249067244E-4
24	7.123004312266811E-5	0.0	0.0	3.561502156133405E-4	2.8492017249067244E-4
25	7.123004312266811E-5	0.0	0.0	4.2738025873600863E-4	2.8492017249067244E-4
26	7.123004312266811E-5	0.0	0.0	4.2738025873600863E-4	2.8492017249067244E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTATCG	35	6.427604E-5	27.431105	8
GTATCGA	40	0.0044815694	20.001848	9
GAACGGT	65	9.428728E-6	19.694126	6
GTATCAA	1680	0.0	19.33443	1
TGCGTAG	80	2.798417E-6	17.9991	13
CTAGCGG	440	0.0	17.817291	29
TCTAGCG	445	0.0	17.617094	28
GTATTAC	85	4.9828213E-6	16.942139	1
TAGCGGC	475	0.0	16.504436	30
AAGACGG	455	0.0	16.177319	5
CGGTCCA	495	0.0	16.161957	10
GGTATCA	630	0.0	16.000908	1
TAGTACG	80	5.6246907E-5	15.999199	2
CAAGACG	470	0.0	15.6599045	4
GCATTCG	405	0.0	15.012663	22
GCGTGTT	75	6.247947E-4	14.932585	30
CCGGTCC	580	0.0	14.897928	9
GTATAGG	140	1.27802195E-8	14.8579855	1
ACCGTCG	360	0.0	14.668022	8
TGTGCGT	120	5.417387E-7	14.666977	11
>>END_MODULE