FastQCFastQC Report
Thu 2 Feb 2017
SRR4063092_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063092_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847044
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA32610.3849859039199853No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC26410.31179017855034685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT22100.26090734365629176No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG15200.17944758477717807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT13660.1612667110563324No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13020.15371102327624067No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12750.1505234674940145No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10450.12337021453430991No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT10130.11959237064426405No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9870.1165228724836018No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9390.11085610664853301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9020.10648797465066749No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8750.10330041886844131No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8740.10318236124687737No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8600.10152955454498232No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8530.10070315119403478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA400.004484484419.9984867
TATACTG1003.266905E-919.196285
ATACCGT1850.019.0233426
TCTAGAT2200.018.9188332
ACCGAGT601.1383927E-418.6652558
TAGCGGC2250.018.48858330
TCTAGCG2250.018.48639928
CGTCGTA1850.018.16186110
CTAGCGG2300.018.08665729
GTCGTAG2050.017.95092211
GCATTAT550.001352559917.476961
TGTTAGG550.00135954317.4635372
TAAGGTA550.001366015917.4511645
GCGAAAG2850.017.4032218
GTGTTAG753.1966647E-517.0885831
CTAGATA2250.017.0623533
ATTTGCC2650.016.90338326
CGAAAGC2950.016.8132819
CCGTCGT2000.016.799729
TACCGTC2000.016.7987297
Produced by FastQC (version 0.11.5)