##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063092_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	847044
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20445455017685	32.0	32.0	32.0	32.0	32.0
2	30.78441025495724	32.0	32.0	32.0	32.0	32.0
3	30.827079821119092	32.0	32.0	32.0	32.0	32.0
4	30.90018582269634	32.0	32.0	32.0	32.0	32.0
5	30.786647446885876	32.0	32.0	32.0	32.0	32.0
6	34.44654350895585	36.0	36.0	36.0	32.0	36.0
7	34.38084562313174	36.0	36.0	36.0	32.0	36.0
8	34.34370941769259	36.0	36.0	36.0	32.0	36.0
9	34.51147283966358	36.0	36.0	36.0	32.0	36.0
10	34.19492257781178	36.0	36.0	36.0	32.0	36.0
11	34.504091877163404	36.0	36.0	36.0	32.0	36.0
12	34.30655314245777	36.0	36.0	36.0	32.0	36.0
13	34.390790797172286	36.0	36.0	36.0	32.0	36.0
14	34.274750780360876	36.0	36.0	36.0	32.0	36.0
15	34.21432652849202	36.0	36.0	36.0	32.0	36.0
16	34.226591534796306	36.0	36.0	36.0	32.0	36.0
17	34.146067972856194	36.0	36.0	36.0	32.0	36.0
18	34.17760470530457	36.0	36.0	36.0	32.0	36.0
19	34.15717365331671	36.0	36.0	36.0	32.0	36.0
20	34.148641629006285	36.0	36.0	36.0	32.0	36.0
21	34.11368004495634	36.0	36.0	36.0	32.0	36.0
22	34.09292905681405	36.0	36.0	36.0	32.0	36.0
23	34.00128918922748	36.0	36.0	36.0	32.0	36.0
24	33.98121703240918	36.0	36.0	36.0	32.0	36.0
25	33.94749623396187	36.0	36.0	36.0	32.0	36.0
26	33.87193935616096	36.0	36.0	36.0	32.0	36.0
27	33.870051614792146	36.0	36.0	36.0	32.0	36.0
28	33.84938326698495	36.0	36.0	36.0	32.0	36.0
29	33.807324058726586	36.0	36.0	36.0	32.0	36.0
30	33.75518272958666	36.0	36.0	36.0	32.0	36.0
31	33.78619174446664	36.0	36.0	36.0	32.0	36.0
32	33.72969763081965	36.0	36.0	36.0	32.0	36.0
33	33.68474010795189	36.0	36.0	36.0	27.0	36.0
34	33.69413749462838	36.0	36.0	36.0	27.0	36.0
35	33.621123577995945	36.0	36.0	36.0	27.0	36.0
36	33.58181865404867	36.0	36.0	36.0	27.0	36.0
37	33.54834341545421	36.0	36.0	36.0	27.0	36.0
38	32.91870316063864	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	1.0
10	12.0
11	7.0
12	1.0
13	7.0
14	60.0
15	172.0
16	251.0
17	274.0
18	384.0
19	515.0
20	753.0
21	1106.0
22	1674.0
23	2575.0
24	4017.0
25	6176.0
26	8988.0
27	13362.0
28	18866.0
29	25318.0
30	34154.0
31	45681.0
32	60335.0
33	88068.0
34	184766.0
35	349517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.057474579597006	16.129573722123848	10.888868925049707	25.92408277322944
2	16.964873175116736	18.906257198240883	36.79427708103728	27.3345925456051
3	20.202259927030568	22.98442610369216	27.04284887771126	29.770465091566006
4	12.827460328142138	14.360616332019301	34.796360377846334	38.01556296199223
5	14.927713147636126	36.01486112849186	33.21606248568548	15.841363238186537
6	35.999329427959545	33.81276873056463	16.265622085431822	13.922279756044004
7	31.280193236714975	29.391507406935176	20.015134987084497	19.31316436926535
8	29.27473404475108	32.29052666872087	18.81561813418713	19.619121152340913
9	27.390108884672916	14.378844993701618	17.75435249450146	40.47669362712401
10	16.337618456765274	26.100214984197894	30.921952826257115	26.640213732779717
11	38.81601938079677	20.86835792138121	20.461521751449784	19.854100946372238
12	24.93689656230889	23.28369274942091	28.00954402601113	23.76986666225907
13	30.50027925144083	18.773489887319386	24.592192531175204	26.134038330064573
14	24.09768146434825	18.913593015938236	24.132512040162606	32.85621347955091
15	25.991254453367908	26.027743434050006	22.361486530370286	25.6195155822118
16	26.91412162816344	25.095461082340943	23.110555386319252	24.879861903176366
17	24.704437569373923	25.238528210093758	24.874241314975322	25.182792905557
18	26.157333616689687	23.760256909762806	25.912466499014158	24.16994297453335
19	25.573992693887092	25.385908694841092	24.57300327523549	24.467095336036326
20	25.785458843031016	23.495110326108133	25.53905658751176	25.180374243349085
21	26.959027803194612	24.1184950453902	23.64139321762148	25.281083933793706
22	25.726939134048664	24.122171112286534	24.729390207042467	25.421499546622332
23	24.60036436238354	23.727358703313854	24.959767828194753	26.712509106107852
24	24.753779939898806	25.125958637627598	24.7230799204161	25.397181502057492
25	25.677927175300486	23.78805572906765	24.388729021600387	26.145288074031477
26	24.626093733394736	24.829430727266285	25.213078748804417	25.331396790534555
27	26.048301877881613	24.328396096650984	24.58980005076951	25.03350197469789
28	25.111897433354468	23.6465753068841	25.310012857431275	25.93151440233016
29	24.41988558879266	24.807632136679477	25.663886084692635	25.108596189835232
30	25.243413060186516	24.458895157948422	25.606333757173704	24.691358024691358
31	24.96484614358358	24.713721869535803	24.45693047505818	25.86450151182244
32	24.95516417762424	24.627647202587077	24.42563511019754	25.99155350959115
33	24.38574285855969	24.21749366556547	24.9959265984854	26.400836877389434
34	25.118988722785502	24.636657854436443	25.39693409955262	24.84741932322544
35	26.14125179035517	24.1227112087746	25.308330745460793	24.42770625540944
36	24.792273041250198	25.25678795142312	24.666639115367133	25.284299891959545
37	26.04026211700809	24.78540645846862	24.767577779089674	24.406753645433614
38	24.84630814876734	24.633199801177586	24.7564602526367	25.76403179741838
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	57.0
2	80.0
3	80.0
4	222.5
5	365.0
6	365.0
7	485.0
8	605.0
9	612.0
10	619.0
11	619.0
12	791.5
13	964.0
14	1249.0
15	1534.0
16	1534.0
17	2268.5
18	3003.0
19	3003.0
20	3637.0
21	4271.0
22	4422.0
23	4573.0
24	4573.0
25	5489.0
26	6405.0
27	6405.0
28	8339.0
29	10273.0
30	12638.5
31	15004.0
32	15004.0
33	19142.5
34	23281.0
35	23281.0
36	27103.5
37	30926.0
38	35957.0
39	40988.0
40	40988.0
41	45075.0
42	49162.0
43	56553.5
44	63945.0
45	63945.0
46	72111.0
47	80277.0
48	80277.0
49	81334.5
50	82392.0
51	81071.5
52	79751.0
53	79751.0
54	77093.5
55	74436.0
56	74436.0
57	72584.5
58	70733.0
59	64690.5
60	58648.0
61	58648.0
62	54895.0
63	51142.0
64	43148.5
65	35155.0
66	35155.0
67	29315.5
68	23476.0
69	23476.0
70	18671.5
71	13867.0
72	10882.0
73	7897.0
74	7897.0
75	6048.0
76	4199.0
77	4199.0
78	3771.0
79	3343.0
80	2630.5
81	1918.0
82	1918.0
83	1829.5
84	1741.0
85	1741.0
86	1202.5
87	664.0
88	567.0
89	470.0
90	470.0
91	336.0
92	202.0
93	175.5
94	149.0
95	149.0
96	137.5
97	126.0
98	126.0
99	276.0
100	426.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12632165507340823
2	0.057021831215379605
3	0.013458568858288352
4	0.0038959015116097864
5	2.3611524312786583E-4
6	7.083457293835976E-4
7	0.0
8	3.541728646917988E-4
9	1.1805762156393292E-4
10	5.902881078196647E-4
11	0.0023611524312786587
12	0.0025972676744065244
13	0.016173894154258812
14	0.010271013076062164
15	0.02514627339311771
16	0.012041877399521159
17	0.02172260236776366
18	0.006375111564452377
19	0.0096807249682425
20	0.006493169186016311
21	0.006493169186016311
22	0.008027918266347439
23	0.010034897832934299
24	0.016410009397386675
25	0.0190072770717932
26	0.02054202615212433
27	0.009326552103550701
28	0.007437630158527774
29	0.01168770453482936
30	0.0031875557822261887
31	0.006257053942888445
32	0.00767374540165564
33	0.010152955454498232
34	0.013576626479852288
35	0.017118355126770273
36	0.016410009397386675
37	0.011097416427009696
38	0.006729284429144176
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	847044.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.935697221475756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.30680953723595	42.46903481895888
2	13.621811555930243	14.966474304891669
3	4.248789214079038	7.0023059346755385
4	1.768465754925368	3.8860759703651424
5	0.8937129808239093	2.4548372858722423
6	0.5358801253272014	1.7663368987188972
7	0.3184697082663526	1.2246748827272442
8	0.210052839432952	0.9231519350079931
9	0.16537201880011146	0.8176344438336407
>10	0.756137122529507	7.589315557332337
>50	0.08078771171906808	3.0743657682755927
>100	0.08509428786435652	9.455585492190153
>500	0.006678285876113511	2.5200850765582974
>1k	0.0019388571898394063	1.850121630592461
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3261	0.3849859039199853	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2641	0.31179017855034685	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2210	0.26090734365629176	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1520	0.17944758477717807	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1366	0.1612667110563324	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1302	0.15371102327624067	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1275	0.1505234674940145	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1045	0.12337021453430991	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1013	0.11959237064426405	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	987	0.1165228724836018	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	939	0.11085610664853301	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	902	0.10648797465066749	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	875	0.10330041886844131	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	874	0.10318236124687737	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	860	0.10152955454498232	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	853	0.10070315119403478	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.3611524312786586E-4	0.0
10	0.0	0.0	0.0	2.3611524312786586E-4	0.0
11	0.0	0.0	0.0	2.3611524312786586E-4	0.0
12	0.0	0.0	0.0	2.3611524312786586E-4	2.3611524312786586E-4
13	0.0	0.0	0.0	2.3611524312786586E-4	2.3611524312786586E-4
14	0.0	0.0	0.0	2.3611524312786586E-4	2.3611524312786586E-4
15	0.0	0.0	0.0	2.3611524312786586E-4	2.3611524312786586E-4
16	0.0	0.0	0.0	3.541728646917988E-4	2.3611524312786586E-4
17	0.0	0.0	0.0	4.722304862557317E-4	2.3611524312786586E-4
18	0.0	0.0	0.0	4.722304862557317E-4	3.541728646917988E-4
19	0.0	0.0	0.0	7.083457293835976E-4	3.541728646917988E-4
20	0.0	0.0	0.0	9.444609725114634E-4	5.902881078196647E-4
21	0.0	0.0	0.0	0.0011805762156393293	7.083457293835976E-4
22	0.0	0.0	0.0	0.001534749080331128	7.083457293835976E-4
23	0.0	0.0	0.0	0.0025972676744065244	7.083457293835976E-4
24	0.0	0.0	0.0	0.0040139591331737196	7.083457293835976E-4
25	0.0	0.0	0.0	0.004604247240993384	7.083457293835976E-4
26	0.0	0.0	0.0	0.006729284429144177	7.083457293835976E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	40	0.0044844844	19.998486	7
TATACTG	100	3.266905E-9	19.19628	5
ATACCGT	185	0.0	19.023342	6
TCTAGAT	220	0.0	18.918833	2
ACCGAGT	60	1.1383927E-4	18.665255	8
TAGCGGC	225	0.0	18.488583	30
TCTAGCG	225	0.0	18.486399	28
CGTCGTA	185	0.0	18.161861	10
CTAGCGG	230	0.0	18.086657	29
GTCGTAG	205	0.0	17.950922	11
GCATTAT	55	0.0013525599	17.47696	1
TGTTAGG	55	0.001359543	17.463537	2
TAAGGTA	55	0.0013660159	17.451164	5
GCGAAAG	285	0.0	17.40322	18
GTGTTAG	75	3.1966647E-5	17.088583	1
CTAGATA	225	0.0	17.062353	3
ATTTGCC	265	0.0	16.903383	26
CGAAAGC	295	0.0	16.81328	19
CCGTCGT	200	0.0	16.79972	9
TACCGTC	200	0.0	16.798729	7
>>END_MODULE