FastQCFastQC Report
Thu 2 Feb 2017
SRR4063092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847044
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA33900.40021533710173257No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC31650.3736523722498477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT18410.21734408129920052No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG16720.19739234325489582No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13420.15843332813879799No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13280.15678052143690294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT12670.14957900652150302No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC10500.12396050264212957No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10470.12360632977743778No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10200.12041877399521159No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10100.11923819777957226No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT9860.11640481486203785No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9590.11321725907981167No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9020.10648797465066749No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8990.1061338017859757No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC8560.10105732405872658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT8540.10082120881559871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG2800.021.71368629
GTATTAG1300.020.9249691
TAGCGGC2950.020.609630
TCTAGCG2950.020.609628
AAGACGG2800.018.8588475
ATCGTTT1550.018.58013229
CAAGACG3000.018.1349744
GCTTAGT450.00884379917.7793851
GCTATAT550.001363417817.4561231
GACGGAC2950.017.3575027
CGGACCA2950.017.3564769
CGCAAGA3050.017.3120172
GCACCGT753.2295626E-517.068216
AGACGGA3200.017.0015356
GCGCAAG3300.016.9712311
AGCGGCG3700.016.86439931
TAGTACT1051.1867451E-716.763424
ACGGACC3200.016.5005178
TAGCACC600.002439721516.0014464
GACGGTA2600.016.0005028
Produced by FastQC (version 0.11.5)