##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063092_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	847044
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13506028022157	32.0	32.0	32.0	32.0	32.0
2	31.201884435755403	32.0	32.0	32.0	32.0	32.0
3	31.33316923323936	32.0	32.0	32.0	32.0	32.0
4	31.440311247113492	32.0	32.0	32.0	32.0	32.0
5	31.344719990933175	32.0	32.0	32.0	32.0	32.0
6	34.88089992963766	36.0	36.0	36.0	36.0	36.0
7	34.91646006582893	36.0	36.0	36.0	36.0	36.0
8	34.841883066287	36.0	36.0	36.0	32.0	36.0
9	34.968322778981964	36.0	36.0	36.0	32.0	36.0
10	34.79059057144611	36.0	36.0	36.0	32.0	36.0
11	34.98018993110157	36.0	36.0	36.0	36.0	36.0
12	34.87133253998611	36.0	36.0	36.0	32.0	36.0
13	34.93661604355854	36.0	36.0	36.0	36.0	36.0
14	34.8683952663616	36.0	36.0	36.0	32.0	36.0
15	34.83258130628397	36.0	36.0	36.0	32.0	36.0
16	34.84569986919215	36.0	36.0	36.0	32.0	36.0
17	34.81144663087159	36.0	36.0	36.0	32.0	36.0
18	34.80354739541275	36.0	36.0	36.0	32.0	36.0
19	34.80082144493084	36.0	36.0	36.0	32.0	36.0
20	34.782364316375535	36.0	36.0	36.0	32.0	36.0
21	34.757291238707786	36.0	36.0	36.0	32.0	36.0
22	34.736654766458415	36.0	36.0	36.0	32.0	36.0
23	34.66266451329565	36.0	36.0	36.0	32.0	36.0
24	34.61811310864607	36.0	36.0	36.0	32.0	36.0
25	34.60901676890457	36.0	36.0	36.0	32.0	36.0
26	34.53152728783865	36.0	36.0	36.0	32.0	36.0
27	34.49985596970169	36.0	36.0	36.0	32.0	36.0
28	34.46798041187943	36.0	36.0	36.0	32.0	36.0
29	34.40660815730942	36.0	36.0	36.0	32.0	36.0
30	34.381311950736915	36.0	36.0	36.0	32.0	36.0
31	34.371173162197	36.0	36.0	36.0	32.0	36.0
32	34.34155014379418	36.0	36.0	36.0	32.0	36.0
33	34.29785111517229	36.0	36.0	36.0	32.0	36.0
34	34.29130954236143	36.0	36.0	36.0	32.0	36.0
35	34.23776214694868	36.0	36.0	36.0	32.0	36.0
36	34.19994592960932	36.0	36.0	36.0	32.0	36.0
37	34.18194686462569	36.0	36.0	36.0	32.0	36.0
38	33.690560348694994	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	8.0
22	61.0
23	191.0
24	535.0
25	1451.0
26	2982.0
27	5904.0
28	10559.0
29	17152.0
30	26309.0
31	37864.0
32	54246.0
33	85654.0
34	200609.0
35	403513.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.427062179444796	17.285186689461703	11.6638409007188	26.623910230374705
2	15.776512200074613	19.95799509824755	36.141215804609914	28.124276897067922
3	19.322254806125773	23.585079405556264	27.3551314925789	29.737534295739067
4	12.367184548545524	15.261853041178695	34.63567245938798	37.7352899508878
5	14.492045277459024	36.589480593688165	32.83501211271197	16.083462016140835
6	36.19829051756705	34.17772478277295	15.863241405364564	13.76074329429543
7	31.34102991229489	29.478786791225474	19.837009455246076	19.343173841233565
8	29.224575570824772	32.55106136809049	18.63769450544261	19.586668555642134
9	27.316517417046924	14.098451621281797	17.559429289918125	41.02560167175316
10	16.0990796140896	26.3711341666706	30.90411741649706	26.62566880274274
11	39.04505425613429	20.735748836187913	20.326894537314597	19.8923023703632
12	24.742692806259903	23.425993044028516	27.8766578280652	23.95465632164639
13	30.568778009170718	18.568811065304754	24.618673882348496	26.243737043176036
14	24.075726880775967	18.893587582227134	24.066636443915545	32.96404909308135
15	26.100415090597416	26.168770453482935	22.11113000033056	25.61968445558908
16	26.898366554748044	25.209552278275982	22.928560972039232	24.963520194936745
17	24.59895825954732	25.407180736774006	24.725988260350114	25.267872743328564
18	26.22591034231988	23.89899462129476	25.583676881012085	24.29141815537327
19	25.78649987485892	25.41343779071689	24.328842421409043	24.471219913015144
20	25.81022945679327	23.506334971973118	25.345908831182324	25.337526740051285
21	27.089619901681612	24.231444883618796	23.365964459933604	25.312970754765985
22	25.68762824039422	24.22512697127179	24.514486884947853	25.572757903386133
23	24.474200247212952	23.8016251831371	24.827311010184843	26.896863559465107
24	24.786079589726153	25.27460202775771	24.439698528057573	25.499619854458565
25	25.764335458766556	23.876001572529375	24.09865850966244	26.261004459041633
26	24.753023455688254	24.88867166286521	24.955020046184142	25.403284835262397
27	26.173492758345496	24.328370190922787	24.422698230552367	25.07543882017935
28	25.129863383720323	23.774325772923248	25.111328337134786	25.984482506221635
29	24.47381718068955	24.714772786301538	25.526064761688882	25.285345271320026
30	25.31403327336006	24.449969541133637	25.35665207474464	24.879345110761662
31	25.029868578255677	24.67900132696767	24.217041853787997	26.07408824098866
32	25.006729284429145	24.592819263225994	24.415024485150713	25.985426967194147
33	24.515845693966313	24.171471611864316	24.875095036385357	26.43758765778401
34	25.088307100929825	24.793399162263118	25.312970754765985	24.805322982041076
35	26.291550379909427	24.09249106303805	24.943686514514006	24.672272042538523
36	24.8173648594406	25.225844230051802	24.567791047454442	25.388999863053158
37	26.139374105713514	24.761759719683983	24.689390397665292	24.409475776937207
38	25.10979358805446	24.391294903216362	24.562478454484065	25.936433054245118
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	26.0
2	33.0
3	33.0
4	139.5
5	246.0
6	246.0
7	368.0
8	490.0
9	512.0
10	534.0
11	534.0
12	714.5
13	895.0
14	1175.5
15	1456.0
16	1456.0
17	2125.0
18	2794.0
19	2794.0
20	3310.0
21	3826.0
22	4151.5
23	4477.0
24	4477.0
25	5384.0
26	6291.0
27	6291.0
28	8357.0
29	10423.0
30	12878.5
31	15334.0
32	15334.0
33	19367.5
34	23401.0
35	23401.0
36	26791.5
37	30182.0
38	35373.0
39	40564.0
40	40564.0
41	44120.0
42	47676.0
43	55164.0
44	62652.0
45	62652.0
46	72278.5
47	81905.0
48	81905.0
49	83359.5
50	84814.0
51	82521.0
52	80228.0
53	80228.0
54	77909.0
55	75590.0
56	75590.0
57	73415.5
58	71241.0
59	64755.0
60	58269.0
61	58269.0
62	54847.0
63	51425.0
64	43251.5
65	35078.0
66	35078.0
67	29243.0
68	23408.0
69	23408.0
70	18646.5
71	13885.0
72	11083.5
73	8282.0
74	8282.0
75	6147.0
76	4012.0
77	4012.0
78	3729.5
79	3447.0
80	2639.0
81	1831.0
82	1831.0
83	1664.5
84	1498.0
85	1498.0
86	988.5
87	479.0
88	370.0
89	261.0
90	261.0
91	160.5
92	60.0
93	39.5
94	19.0
95	19.0
96	16.0
97	13.0
98	13.0
99	9.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008972379238858902
2	0.0
3	0.0
4	4.7223048625573167E-4
5	0.0
6	4.7223048625573167E-4
7	1.1805762156393292E-4
8	0.0028333829175343902
9	0.003423671025354055
10	9.444609725114633E-4
11	0.005076477727249116
12	2.3611524312786583E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	2.3611524312786583E-4
23	1.1805762156393292E-4
24	0.0
25	1.1805762156393292E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	847044.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.734054779392494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67073783581193	39.90424834230754
2	14.447148382747452	15.237134284436316
3	4.6519962331382	7.359558725755121
4	1.9448288643368044	4.102348474739229
5	0.9735052119155492	2.5668438586589333
6	0.5621041786554679	1.7785219529365723
7	0.3465965313456787	1.2794208329231311
8	0.2272357387385755	0.9586449515580608
9	0.1728432185480259	0.8203251379642295
>10	0.8158490954272488	7.877198188413797
>50	0.0850302240925705	3.1601324079818043
>100	0.09042740764394828	9.555356897764147
>500	0.009222695798920355	3.249412081699417
>1k	0.002474381799710339	2.15085386286182
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3390	0.40021533710173257	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3165	0.3736523722498477	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1841	0.21734408129920052	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1672	0.19739234325489582	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1342	0.15843332813879799	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1328	0.15678052143690294	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1267	0.14957900652150302	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	1050	0.12396050264212957	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1047	0.12360632977743778	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1020	0.12041877399521159	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1010	0.11923819777957226	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	986	0.11640481486203785	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	959	0.11321725907981167	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	902	0.10648797465066749	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	899	0.1061338017859757	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	856	0.10105732405872658	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	854	0.10082120881559871	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1805762156393293E-4
2	0.0	0.0	0.0	1.1805762156393293E-4	1.1805762156393293E-4
3	0.0	0.0	0.0	1.1805762156393293E-4	1.1805762156393293E-4
4	0.0	0.0	0.0	1.1805762156393293E-4	1.1805762156393293E-4
5	0.0	0.0	0.0	1.1805762156393293E-4	1.1805762156393293E-4
6	0.0	0.0	0.0	1.1805762156393293E-4	1.1805762156393293E-4
7	0.0	0.0	0.0	1.1805762156393293E-4	1.1805762156393293E-4
8	0.0	0.0	0.0	1.1805762156393293E-4	1.1805762156393293E-4
9	0.0	0.0	0.0	2.3611524312786586E-4	1.1805762156393293E-4
10	0.0	0.0	0.0	2.3611524312786586E-4	1.1805762156393293E-4
11	0.0	0.0	0.0	2.3611524312786586E-4	1.1805762156393293E-4
12	0.0	0.0	0.0	2.3611524312786586E-4	4.722304862557317E-4
13	0.0	0.0	0.0	2.3611524312786586E-4	7.083457293835976E-4
14	0.0	0.0	0.0	3.541728646917988E-4	7.083457293835976E-4
15	0.0	0.0	0.0	3.541728646917988E-4	8.264033509475304E-4
16	0.0	0.0	0.0	4.722304862557317E-4	0.0010625185940753962
17	0.0	0.0	0.0	5.902881078196647E-4	0.0011805762156393293
18	0.0	0.0	0.0	5.902881078196647E-4	0.0011805762156393293
19	0.0	0.0	0.0	7.083457293835976E-4	0.0011805762156393293
20	0.0	0.0	0.0	9.444609725114634E-4	0.0012986338372032622
21	0.0	0.0	0.0	0.0011805762156393293	0.0012986338372032622
22	0.0	0.0	1.1805762156393293E-4	0.0016528067018950609	0.0012986338372032622
23	0.0	0.0	1.1805762156393293E-4	0.002951440539098323	0.0012986338372032622
24	0.0	0.0	1.1805762156393293E-4	0.0038959015116097864	0.0012986338372032622
25	0.0	0.0	1.1805762156393293E-4	0.004486189619429451	0.0012986338372032622
26	0.0	0.0	1.1805762156393293E-4	0.006493169186016311	0.0012986338372032622
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	280	0.0	21.713686	29
GTATTAG	130	0.0	20.924969	1
TAGCGGC	295	0.0	20.6096	30
TCTAGCG	295	0.0	20.6096	28
AAGACGG	280	0.0	18.858847	5
ATCGTTT	155	0.0	18.580132	29
CAAGACG	300	0.0	18.134974	4
GCTTAGT	45	0.008843799	17.779385	1
GCTATAT	55	0.0013634178	17.456123	1
GACGGAC	295	0.0	17.357502	7
CGGACCA	295	0.0	17.356476	9
CGCAAGA	305	0.0	17.312017	2
GCACCGT	75	3.2295626E-5	17.06821	6
AGACGGA	320	0.0	17.001535	6
GCGCAAG	330	0.0	16.971231	1
AGCGGCG	370	0.0	16.864399	31
TAGTACT	105	1.1867451E-7	16.76342	4
ACGGACC	320	0.0	16.500517	8
TAGCACC	60	0.0024397215	16.001446	4
GACGGTA	260	0.0	16.000502	8
>>END_MODULE