FastQCFastQC Report
Thu 2 Feb 2017
SRR4063091_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063091_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087619
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40460.19380931099017587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24180.11582573256901763No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24130.11558622526428435No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23490.11252053176369826No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22200.10634124330157946No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21650.1037066629495133No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21320.10212591473827361No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21130.1012157869802871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32700.017.3849331
TAGGACG652.1053718E-417.2265534
TCTAGCG4900.015.01960928
GTACTAA1308.23311E-814.7819021
GTCCTAT7200.014.6792511
TTTCGTT6200.014.19279428
TATACAG3050.014.1604685
CCTATTC7800.014.1517383
GGTATCA14600.014.1491151
CTAGCGG5000.014.079926529
CATACCG800.001020320913.99657255
ATAGCGT3800.013.89133556
CTTTCGT6350.013.85719927
GTCCTAA1851.8007995E-1013.84970951
TTCGTTC6600.013.818109529
CGACTTT6800.013.64666124
TAGCGTA3900.013.5377427
CGACGGT7000.013.4849977
TAGCGGC5350.013.45787430
CGCTTCG4300.013.39527932
Produced by FastQC (version 0.11.5)