##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063091_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2087619
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.273810019931798	32.0	32.0	32.0	32.0	32.0
2	30.877159098475346	32.0	32.0	32.0	32.0	32.0
3	30.922568246408947	32.0	32.0	32.0	32.0	32.0
4	30.996127645897072	32.0	32.0	32.0	32.0	32.0
5	30.890784669041622	32.0	32.0	32.0	32.0	32.0
6	34.55711554646705	36.0	36.0	36.0	32.0	36.0
7	34.49799747942512	36.0	36.0	36.0	32.0	36.0
8	34.49694843743039	36.0	36.0	36.0	32.0	36.0
9	34.635130260837826	36.0	36.0	36.0	32.0	36.0
10	34.34572304620718	36.0	36.0	36.0	32.0	36.0
11	34.61770514638926	36.0	36.0	36.0	32.0	36.0
12	34.43443128271969	36.0	36.0	36.0	32.0	36.0
13	34.517608337536686	36.0	36.0	36.0	32.0	36.0
14	34.41530614542213	36.0	36.0	36.0	32.0	36.0
15	34.35973134944643	36.0	36.0	36.0	32.0	36.0
16	34.37856620389065	36.0	36.0	36.0	32.0	36.0
17	34.31080096511864	36.0	36.0	36.0	32.0	36.0
18	34.33090951940943	36.0	36.0	36.0	32.0	36.0
19	34.319093187023114	36.0	36.0	36.0	32.0	36.0
20	34.3080868683414	36.0	36.0	36.0	32.0	36.0
21	34.29567895291238	36.0	36.0	36.0	32.0	36.0
22	34.265518277041934	36.0	36.0	36.0	32.0	36.0
23	34.213663987537956	36.0	36.0	36.0	32.0	36.0
24	34.19090839851525	36.0	36.0	36.0	32.0	36.0
25	34.17578063813368	36.0	36.0	36.0	32.0	36.0
26	34.125503264724074	36.0	36.0	36.0	32.0	36.0
27	34.131073246602945	36.0	36.0	36.0	32.0	36.0
28	34.11714781289115	36.0	36.0	36.0	32.0	36.0
29	34.084418181670124	36.0	36.0	36.0	32.0	36.0
30	34.06865045777031	36.0	36.0	36.0	32.0	36.0
31	34.074790467034454	36.0	36.0	36.0	32.0	36.0
32	34.045139941723086	36.0	36.0	36.0	32.0	36.0
33	33.99938015509535	36.0	36.0	36.0	32.0	36.0
34	34.01169274661708	36.0	36.0	36.0	32.0	36.0
35	33.974248653609685	36.0	36.0	36.0	32.0	36.0
36	33.92841318267366	36.0	36.0	36.0	32.0	36.0
37	33.93196651304668	36.0	36.0	36.0	32.0	36.0
38	33.36015958850729	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	1.0
7	4.0
8	0.0
9	5.0
10	15.0
11	19.0
12	9.0
13	8.0
14	206.0
15	417.0
16	644.0
17	726.0
18	929.0
19	1251.0
20	1584.0
21	2333.0
22	3555.0
23	5695.0
24	8662.0
25	13183.0
26	19357.0
27	27732.0
28	39464.0
29	53089.0
30	72216.0
31	97839.0
32	134272.0
33	201712.0
34	450822.0
35	951869.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.30906112160102	17.29181664143174	11.252795465674344	26.146326771292898
2	16.83673591650719	19.87411472688992	37.468002681999494	25.82114667460339
3	18.662872445667666	24.34318537308642	28.600809146372136	28.39313303487378
4	12.790842497159357	15.384257955235691	36.3675034346384	35.45739611296655
5	14.78103002708833	36.276276995518806	33.68585682704905	15.256836150343814
6	33.683301909929796	35.722422131273184	17.11215089365094	13.48212506514608
7	29.72649702843287	30.52808007591424	21.534868191944987	18.21055470370791
8	27.787190441906617	32.65025653701141	19.961362570553067	19.601190450528904
9	27.668586083723373	14.24258231769969	18.647757031595415	39.44107456698153
10	15.785986948717937	26.36951999199085	31.83658258171661	26.007910477574608
11	37.20318644944649	21.331200091972082	22.53426199589	18.931351462691428
12	24.948780234272046	23.642143292713598	28.755928535193735	22.653147937820616
13	29.367311290970115	19.45131398534193	25.328580175287474	25.85279454840048
14	23.784893385646466	19.678600000958117	25.201563645247983	31.334942968147434
15	25.12108773656368	26.755730256434333	22.49891357624826	25.624268430753723
16	25.833749325709093	25.67800369650346	23.50393651748749	24.984310460299955
17	24.22550138008585	25.6993496995241	25.092888090153792	24.982260830236267
18	25.045508894833258	24.73221608198116	25.935471261372435	24.28680376181315
19	25.700085271095013	24.887279277207707	25.479625956904563	23.933009494792714
20	25.82906547110636	24.187102185819313	24.818862362221623	25.164969980852703
21	27.007830411659484	24.247066589636802	24.40601254513768	24.33909045356603
22	25.99157161136285	24.141196827354083	24.867530966926935	24.99970059435613
23	24.43036113018038	23.962079486677087	25.56511174804867	26.04244763509386
24	24.872898489346085	24.903032997526	25.16772800180903	25.056340511318886
25	24.940507987763834	24.33785043034991	25.22873343218725	25.492908149699005
26	24.85823302485402	25.122171607510058	25.501478516003047	24.518116851632886
27	25.44903366680607	24.704768118837208	24.676791092574735	25.169407121781983
28	24.664395048812	24.613329156085822	25.587557167692054	25.13471862741012
29	24.650009700993813	24.919625469779607	25.431310975536732	24.99905385368985
30	24.61010869580838	24.991269749646598	25.604471037843542	24.79415051670148
31	25.19241796338514	24.79888747998216	24.566984732550168	25.44170982408253
32	24.84440569490486	24.823710885852798	24.541744112518444	25.790139306723898
33	24.547650368826034	24.39674739416911	25.273757158310158	25.781845078694705
34	25.31651330656578	24.50562985752769	25.261898682206603	24.915958153699926
35	25.989387002623545	24.332375774463266	25.195903513327572	24.482333709585617
36	24.81805573188522	25.059083080416706	24.901991314200302	25.22086987349777
37	25.6596193571382	24.821741329950523	24.859635070345345	24.659004242565928
38	24.66423887758348	24.673915542832177	25.40455167339243	25.25729390619192
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	230.0
1	236.5
2	243.0
3	243.0
4	551.0
5	859.0
6	859.0
7	1038.5
8	1218.0
9	1180.0
10	1142.0
11	1142.0
12	1597.5
13	2053.0
14	2868.0
15	3683.0
16	3683.0
17	5688.0
18	7693.0
19	7693.0
20	9853.0
21	12013.0
22	13634.0
23	15255.0
24	15255.0
25	18313.0
26	21371.0
27	21371.0
28	27047.0
29	32723.0
30	39666.5
31	46610.0
32	46610.0
33	59075.5
34	71541.0
35	71541.0
36	80479.0
37	89417.0
38	101793.0
39	114169.0
40	114169.0
41	123284.0
42	132399.0
43	149041.0
44	165683.0
45	165683.0
46	169618.5
47	173554.0
48	173554.0
49	184335.0
50	195116.0
51	197449.5
52	199783.0
53	199783.0
54	189932.0
55	180081.0
56	180081.0
57	174208.5
58	168336.0
59	150705.0
60	133074.0
61	133074.0
62	124797.5
63	116521.0
64	95983.5
65	75446.0
66	75446.0
67	62597.5
68	49749.0
69	49749.0
70	40083.5
71	30418.0
72	23703.5
73	16989.0
74	16989.0
75	12926.5
76	8864.0
77	8864.0
78	8305.0
79	7746.0
80	6043.5
81	4341.0
82	4341.0
83	4454.5
84	4568.0
85	4568.0
86	3050.5
87	1533.0
88	1285.0
89	1037.0
90	1037.0
91	767.0
92	497.0
93	439.5
94	382.0
95	382.0
96	352.0
97	322.0
98	322.0
99	641.0
100	960.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12195711957018976
2	0.05345803041646967
3	0.011208941861517835
4	0.003017792039639417
5	1.9160584378662967E-4
6	1.4370438283997224E-4
7	0.0
8	2.874087656799445E-4
9	2.874087656799445E-4
10	3.8321168757325934E-4
11	0.0013891423674530648
12	0.0022034672035462412
13	0.01460994558873051
14	0.00905337611891825
15	0.023759124629542078
16	0.011544252088144436
17	0.020597628207062686
18	0.005508668008865602
19	0.007616332290518528
20	0.005652372391705574
21	0.005604470930758917
22	0.007185219141998612
23	0.009580292189331482
24	0.014897354354410456
25	0.017436131784583298
26	0.018729471230143048
27	0.009197080501758223
28	0.005748175313598889
29	0.009771898033118112
30	0.0023950730473328704
31	0.005077554860345686
32	0.006658303071585381
33	0.008861770275131622
34	0.012741788611810871
35	0.01757983616742327
36	0.014562044127783853
37	0.009723996572171455
38	0.0058918796964388615
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2087619.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.64172178645311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.32037767210417	46.06126207672618
2	11.268190979787649	12.76499477027505
3	3.164316976124489	5.376970854173817
4	1.3590997460772845	3.0792699878939445
5	0.7249507048254425	2.0531228065807903
6	0.4570047118513719	1.553132024627015
7	0.3189523143001029	1.2646205774812247
8	0.22678200418581773	1.0276258549813868
9	0.1694366346055618	0.8637464445985029
>10	0.8589704814739526	8.597898108029531
>50	0.05903204774233687	2.3517938522268738
>100	0.059620351521027244	7.6234123417746265
>500	0.009268755889197651	3.773292772801471
>1k	0.003996619511855867	3.608857527829562
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4046	0.19380931099017587	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2418	0.11582573256901763	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2413	0.11558622526428435	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2349	0.11252053176369826	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2220	0.10634124330157946	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2165	0.1037066629495133	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2132	0.10212591473827361	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2113	0.1012157869802871	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3950730473328705E-4
2	0.0	0.0	0.0	0.0	2.3950730473328705E-4
3	0.0	0.0	0.0	0.0	2.3950730473328705E-4
4	0.0	0.0	0.0	0.0	2.3950730473328705E-4
5	0.0	0.0	0.0	0.0	2.874087656799445E-4
6	0.0	0.0	0.0	0.0	2.874087656799445E-4
7	0.0	0.0	0.0	0.0	2.874087656799445E-4
8	0.0	0.0	0.0	0.0	2.874087656799445E-4
9	0.0	0.0	0.0	4.790146094665741E-5	2.874087656799445E-4
10	0.0	0.0	0.0	4.790146094665741E-5	2.874087656799445E-4
11	0.0	0.0	0.0	4.790146094665741E-5	3.832116875732593E-4
12	0.0	0.0	0.0	4.790146094665741E-5	7.185219141998612E-4
13	0.0	0.0	0.0	9.580292189331482E-5	7.185219141998612E-4
14	0.0	0.0	0.0	9.580292189331482E-5	7.185219141998612E-4
15	4.790146094665741E-5	0.0	0.0	9.580292189331482E-5	8.14324836093176E-4
16	4.790146094665741E-5	0.0	0.0	1.9160584378662964E-4	8.622262970398334E-4
17	4.790146094665741E-5	0.0	0.0	2.874087656799445E-4	8.622262970398334E-4
18	4.790146094665741E-5	0.0	0.0	3.832116875732593E-4	9.101277579864908E-4
19	4.790146094665741E-5	0.0	0.0	4.311131485199167E-4	9.101277579864908E-4
20	4.790146094665741E-5	0.0	0.0	4.790146094665741E-4	0.0011017336017731204
21	4.790146094665741E-5	0.0	0.0	4.790146094665741E-4	0.001149635062719778
22	4.790146094665741E-5	0.0	0.0	6.227189923065464E-4	0.001149635062719778
23	4.790146094665741E-5	0.0	0.0	0.0011975365236664352	0.001149635062719778
24	4.790146094665741E-5	0.0	0.0	0.001628649672186352	0.001149635062719778
25	4.790146094665741E-5	0.0	0.0	0.0019639598988129537	0.001149635062719778
26	4.790146094665741E-5	0.0	0.0	0.0023950730473328704	0.0011975365236664352
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3270	0.0	17.384933	1
TAGGACG	65	2.1053718E-4	17.226553	4
TCTAGCG	490	0.0	15.019609	28
GTACTAA	130	8.23311E-8	14.781902	1
GTCCTAT	720	0.0	14.679251	1
TTTCGTT	620	0.0	14.192794	28
TATACAG	305	0.0	14.160468	5
CCTATTC	780	0.0	14.151738	3
GGTATCA	1460	0.0	14.149115	1
CTAGCGG	500	0.0	14.0799265	29
CATACCG	80	0.0010203209	13.9965725	5
ATAGCGT	380	0.0	13.8913355	6
CTTTCGT	635	0.0	13.857199	27
GTCCTAA	185	1.8007995E-10	13.8497095	1
TTCGTTC	660	0.0	13.8181095	29
CGACTTT	680	0.0	13.646661	24
TAGCGTA	390	0.0	13.537742	7
CGACGGT	700	0.0	13.484997	7
TAGCGGC	535	0.0	13.457874	30
CGCTTCG	430	0.0	13.395279	32
>>END_MODULE