FastQCFastQC Report
Thu 2 Feb 2017
SRR4063091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087619
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42120.201760953507321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27400.13125000299384132No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24600.11783759392877723No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24290.11635264863943086No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22940.10988595141163211No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22920.1097901484897388No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21810.10447308632465982No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21390.1024612249649002No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20890.10006615191756733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29950.017.6844861
CTAGCGG5500.016.87190629
AACCGCG3250.016.2484767
ACCGTCG4900.016.0022898
TCTAGCG5800.015.99922328
TAGCGGC5850.015.86247630
GGCGGGT2550.015.68551132
GTATTAC2250.015.6459311
CGCGGTC3400.015.52977310
CGTCGTA5300.015.39658410
AATATCG756.240604E-414.9354695
TACCGTC5150.014.9147547
ATACCGT5400.014.8169346
GAACCGC4150.014.65269856
GGTCCTA3950.014.58156913
ATCGTTT4150.014.26436629
GGTATCA13700.014.2495291
TAAACGC4500.014.2215328
TAGCGTA4100.014.0507897
CGTATAT4100.014.04910610
Produced by FastQC (version 0.11.5)