##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063091_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2087619
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23387265588213	32.0	32.0	32.0	32.0	32.0
2	31.281800462632308	32.0	32.0	32.0	32.0	32.0
3	31.36433707491645	32.0	32.0	32.0	32.0	32.0
4	31.46628671227844	32.0	32.0	32.0	32.0	32.0
5	31.384444192163418	32.0	32.0	32.0	32.0	32.0
6	34.92116760769087	36.0	36.0	36.0	36.0	36.0
7	34.945314734154074	36.0	36.0	36.0	36.0	36.0
8	34.87966242882442	36.0	36.0	36.0	32.0	36.0
9	34.99519500445244	36.0	36.0	36.0	36.0	36.0
10	34.843759325815675	36.0	36.0	36.0	32.0	36.0
11	35.02384917937612	36.0	36.0	36.0	36.0	36.0
12	34.92437269444281	36.0	36.0	36.0	32.0	36.0
13	34.97133145463803	36.0	36.0	36.0	36.0	36.0
14	34.92448574189064	36.0	36.0	36.0	32.0	36.0
15	34.88467052656639	36.0	36.0	36.0	32.0	36.0
16	34.90341005710333	36.0	36.0	36.0	32.0	36.0
17	34.87516783474379	36.0	36.0	36.0	32.0	36.0
18	34.869003874749176	36.0	36.0	36.0	32.0	36.0
19	34.86948672147552	36.0	36.0	36.0	32.0	36.0
20	34.861204079863235	36.0	36.0	36.0	32.0	36.0
21	34.85623957245072	36.0	36.0	36.0	32.0	36.0
22	34.83839196711661	36.0	36.0	36.0	32.0	36.0
23	34.78073824773582	36.0	36.0	36.0	32.0	36.0
24	34.760503712602734	36.0	36.0	36.0	32.0	36.0
25	34.73931210627993	36.0	36.0	36.0	32.0	36.0
26	34.67042836839481	36.0	36.0	36.0	32.0	36.0
27	34.66390754251614	36.0	36.0	36.0	32.0	36.0
28	34.63438778819315	36.0	36.0	36.0	32.0	36.0
29	34.601730488178156	36.0	36.0	36.0	32.0	36.0
30	34.58080952510971	36.0	36.0	36.0	32.0	36.0
31	34.58018776414662	36.0	36.0	36.0	32.0	36.0
32	34.54999547331194	36.0	36.0	36.0	32.0	36.0
33	34.52246267158902	36.0	36.0	36.0	32.0	36.0
34	34.51651666324171	36.0	36.0	36.0	32.0	36.0
35	34.48471488331923	36.0	36.0	36.0	32.0	36.0
36	34.46004706797552	36.0	36.0	36.0	32.0	36.0
37	34.45830441282629	36.0	36.0	36.0	32.0	36.0
38	34.03126528355988	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	9.0
21	35.0
22	139.0
23	423.0
24	1324.0
25	3126.0
26	6702.0
27	13030.0
28	23194.0
29	37166.0
30	56878.0
31	82495.0
32	119176.0
33	193713.0
34	476893.0
35	1073314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65028750403004	18.498368103843497	11.978879484001764	26.872464908124698
2	15.742575632814226	20.931549291321836	36.81715868652278	26.50871638934116
3	17.68062084125504	25.13265112072653	28.924913980951505	28.261814057066925
4	12.245068058493516	16.341127870259665	36.43102946135688	34.982774609889944
5	14.214724252580812	36.889333627767925	33.3942001813551	15.501741938296155
6	33.63191967867639	36.186269977941336	16.78044083798976	13.401369505392518
7	29.5206001120899	30.755936214139613	21.493765597980467	18.22969807579002
8	27.65898740689538	32.99164435604825	19.700072476576842	19.649295760479525
9	27.494253996813473	14.172005491636561	18.511663021224045	39.822077490325924
10	15.552953946571105	26.668269785787203	31.810162593642353	25.968613673999343
11	37.33329309373527	21.323681578312005	22.46821071752952	18.874814610423204
12	24.746217208944955	23.855103342958365	28.645888220608047	22.752791227488633
13	29.345209724973238	19.510589835769526	25.262751992105827	25.881448447151413
14	23.59670207863699	19.667391256446344	25.23967507465446	31.4962315902622
15	25.118232780981586	26.90898099701143	22.307997771624034	25.664788450382947
16	25.808313589938386	25.87815395345317	23.302970179410217	25.010562277198222
17	24.190812595593354	25.835509257196836	25.02041799772851	24.9532601494813
18	25.18194172404064	24.801747828507022	25.740520660139616	24.275789787312725
19	25.65429803043563	24.937261061525113	25.41704209436684	23.99139881367242
20	25.86137604610803	24.198093617657246	24.801747828507022	25.138782507727704
21	26.911223136822098	24.353041938747356	24.358885925380914	24.37684899904963
22	25.884225042979587	24.26496405713878	24.871396552723464	24.979414347158173
23	24.383950105742677	23.95044105354675	25.513612423746718	26.151996416963858
24	24.820429398276218	24.956182138599043	25.11550239770763	25.107886065417105
25	24.950817174973018	24.316458127656436	25.29043853308482	25.442286164285726
26	24.932854127118024	25.122400208083945	25.398504228980478	24.54624143581755
27	25.476368510124225	24.681299299632066	24.601399586226783	25.240932604016926
28	24.596154758123966	24.579437148253586	25.60428890520732	25.22011918841513
29	24.759498739952072	24.75058906821599	25.470404321861412	25.019507869970525
30	24.6845808550315	24.89822137085359	25.602468649691346	24.814729124423565
31	25.207569005647102	24.745942626504167	24.63035640123988	25.41613196660885
32	24.95196681003574	24.72108176827285	24.584993717723396	25.741957703968016
33	24.577808498581398	24.325367799392513	25.284211343161754	25.812612358864335
34	25.25245267455412	24.396309863054512	25.3816908161882	24.96954664620316
35	25.98563243580366	24.306638328162368	25.136195828836584	24.571533407197386
36	24.950481864746394	24.96240932852211	24.82929116855135	25.257817638180146
37	25.703157520601223	24.831925748903416	24.793556678685142	24.671360051810222
38	24.73753566865953	24.549665958842066	25.356039924947922	25.356758447550487
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	137.0
1	141.0
2	145.0
3	145.0
4	484.0
5	823.0
6	823.0
7	956.5
8	1090.0
9	1049.0
10	1008.0
11	1008.0
12	1513.0
13	2018.0
14	2889.0
15	3760.0
16	3760.0
17	5848.0
18	7936.0
19	7936.0
20	10031.5
21	12127.0
22	13750.0
23	15373.0
24	15373.0
25	18536.0
26	21699.0
27	21699.0
28	27218.5
29	32738.0
30	40031.0
31	47324.0
32	47324.0
33	60039.5
34	72755.0
35	72755.0
36	81438.5
37	90122.0
38	102484.0
39	114846.0
40	114846.0
41	123930.0
42	133014.0
43	149534.5
44	166055.0
45	166055.0
46	169165.5
47	172276.0
48	172276.0
49	184275.0
50	196274.0
51	197663.5
52	199053.0
53	199053.0
54	189550.5
55	180048.0
56	180048.0
57	174533.5
58	169019.0
59	151033.5
60	133048.0
61	133048.0
62	124861.0
63	116674.0
64	95793.5
65	74913.0
66	74913.0
67	62482.0
68	50051.0
69	50051.0
70	40194.0
71	30337.0
72	23688.0
73	17039.0
74	17039.0
75	12739.0
76	8439.0
77	8439.0
78	8056.0
79	7673.0
80	5852.5
81	4032.0
82	4032.0
83	3939.5
84	3847.0
85	3847.0
86	2402.0
87	957.0
88	834.0
89	711.0
90	711.0
91	435.5
92	160.0
93	105.0
94	50.0
95	50.0
96	38.0
97	26.0
98	26.0
99	24.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00814324836093176
2	0.0
3	0.0
4	1.4370438283997224E-4
5	9.580292189331484E-5
6	1.9160584378662967E-4
7	4.311131485199167E-4
8	0.002299270125439556
9	0.0038800183366792503
10	0.0010538321408264631
11	0.0058918796964388615
12	3.353102266266019E-4
13	1.9160584378662967E-4
14	4.790146094665742E-5
15	0.0
16	4.790146094665742E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.4370438283997224E-4
22	0.0
23	1.9160584378662967E-4
24	0.0
25	0.0
26	0.0
27	9.580292189331484E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	9.580292189331484E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2087619.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.94936336161877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.16488704031477	44.05009506821381
2	12.004125910815503	13.19238153024048
3	3.344571037623023	5.513461477052816
4	1.409771325803815	3.098641473535395
5	0.764808708348657	2.101287580859034
6	0.49033776744523694	1.6166248891963948
7	0.33657619497301616	1.2946253345510317
8	0.23616919248890147	1.038187741831419
9	0.17934671125245882	0.8869488843890414
>10	0.9281925954744727	8.971312034143725
>50	0.06364686868468016	2.439880556762396
>100	0.06258151955647262	7.711211747067822
>500	0.01007771713576046	3.9028333571884364
>1k	0.004907410083500744	4.182508324968206
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4212	0.201760953507321	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2740	0.13125000299384132	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2460	0.11783759392877723	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2429	0.11635264863943086	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2294	0.10988595141163211	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2292	0.1097901484897388	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2181	0.10447308632465982	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2139	0.1024612249649002	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2089	0.10006615191756733	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.9160584378662964E-4
7	0.0	0.0	0.0	0.0	1.9160584378662964E-4
8	0.0	0.0	0.0	0.0	1.9160584378662964E-4
9	0.0	0.0	0.0	0.0	1.9160584378662964E-4
10	0.0	0.0	0.0	0.0	1.9160584378662964E-4
11	0.0	0.0	0.0	0.0	1.9160584378662964E-4
12	0.0	0.0	0.0	0.0	1.9160584378662964E-4
13	0.0	0.0	0.0	0.0	1.9160584378662964E-4
14	0.0	0.0	0.0	0.0	2.3950730473328705E-4
15	0.0	0.0	0.0	0.0	3.832116875732593E-4
16	0.0	0.0	0.0	9.580292189331482E-5	3.832116875732593E-4
17	0.0	0.0	0.0	9.580292189331482E-5	3.832116875732593E-4
18	0.0	0.0	0.0	1.9160584378662964E-4	3.832116875732593E-4
19	0.0	0.0	0.0	2.3950730473328705E-4	3.832116875732593E-4
20	0.0	0.0	0.0	2.874087656799445E-4	4.790146094665741E-4
21	0.0	0.0	0.0	2.874087656799445E-4	4.790146094665741E-4
22	0.0	0.0	0.0	4.311131485199167E-4	4.790146094665741E-4
23	0.0	0.0	0.0	8.14324836093176E-4	4.790146094665741E-4
24	0.0	0.0	0.0	0.0013412409065064075	4.790146094665741E-4
25	0.0	0.0	0.0	0.0015328467502930372	4.790146094665741E-4
26	0.0	0.0	0.0	0.0019160584378662964	5.269160704132316E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2995	0.0	17.684486	1
CTAGCGG	550	0.0	16.871906	29
AACCGCG	325	0.0	16.248476	7
ACCGTCG	490	0.0	16.002289	8
TCTAGCG	580	0.0	15.999223	28
TAGCGGC	585	0.0	15.862476	30
GGCGGGT	255	0.0	15.685511	32
GTATTAC	225	0.0	15.645931	1
CGCGGTC	340	0.0	15.529773	10
CGTCGTA	530	0.0	15.396584	10
AATATCG	75	6.240604E-4	14.935469	5
TACCGTC	515	0.0	14.914754	7
ATACCGT	540	0.0	14.816934	6
GAACCGC	415	0.0	14.6526985	6
GGTCCTA	395	0.0	14.581569	13
ATCGTTT	415	0.0	14.264366	29
GGTATCA	1370	0.0	14.249529	1
TAAACGC	450	0.0	14.22153	28
TAGCGTA	410	0.0	14.050789	7
CGTATAT	410	0.0	14.049106	10
>>END_MODULE