FastQCFastQC Report
Thu 2 Feb 2017
SRR4063090_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063090_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069945
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26860.2510409413567987No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19550.1827196725065307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18540.17327993494992733No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18450.1724387702171607No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17350.1621578679277907No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16290.15225081663076137No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15780.1474842164784171No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15650.14626920075330974No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15190.14196991434139136No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15170.14178298884522103No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14350.13411904350223608No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14060.13140862380776583No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13880.12972629434223254No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13800.12897859235755108No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT12970.12122118426648099No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12940.12094079602222543No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT12630.1180434508315848No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA12530.11710882335073299No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC11960.11178144670987761No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11880.11103374472519614No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC11810.11037950548859989No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT11660.10897756426732216No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT11440.10692138380944814No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG11370.10626714457285188No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT11180.10449135235923342No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC11100.10374365037455195No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG350.0020699622.8504685
TTAGACC400.004489650519.9950924
TAGATCG400.004490865419.9941585
CAAGACG4950.018.4197224
CTAGCGG3800.017.6848329
TAGCGGC3850.017.45515830
TCTAGCG3850.017.45515828
GTTATTC1950.017.2273463
CGCAAGA5350.017.0513132
GGCGTTA1700.016.9417731
AAGACGG5300.016.9007235
GTCCTAA957.620929E-716.8568821
CGTTATT1900.016.8466342
CGACGAA957.6883407E-716.84269532
ATAATAG1354.4383341E-1016.5892963
CGCGCCA1456.91216E-1116.55230510
GCGCAAG5700.016.0140381
CATCGTT2400.016.00056328
ACGCTTA805.6208337E-515.99981317
ATTAGCC600.002444996315.9968213
Produced by FastQC (version 0.11.5)