##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063090_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1069945
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266245461215295	32.0	32.0	32.0	32.0	32.0
2	30.92240535728472	32.0	32.0	32.0	32.0	32.0
3	30.965158022141324	32.0	32.0	32.0	32.0	32.0
4	31.031639944109276	32.0	32.0	32.0	32.0	32.0
5	30.958650210992154	32.0	32.0	32.0	32.0	32.0
6	34.61809345340181	36.0	36.0	36.0	32.0	36.0
7	34.53799494366533	36.0	36.0	36.0	32.0	36.0
8	34.52137259391838	36.0	36.0	36.0	32.0	36.0
9	34.64244330316044	36.0	36.0	36.0	32.0	36.0
10	34.39868124062452	36.0	36.0	36.0	32.0	36.0
11	34.62272266331447	36.0	36.0	36.0	32.0	36.0
12	34.45532714298398	36.0	36.0	36.0	32.0	36.0
13	34.53272084079088	36.0	36.0	36.0	32.0	36.0
14	34.435167228222014	36.0	36.0	36.0	32.0	36.0
15	34.38136633191426	36.0	36.0	36.0	32.0	36.0
16	34.39809429456654	36.0	36.0	36.0	32.0	36.0
17	34.32110902896878	36.0	36.0	36.0	32.0	36.0
18	34.34563552332129	36.0	36.0	36.0	32.0	36.0
19	34.33835103673553	36.0	36.0	36.0	32.0	36.0
20	34.31793129553388	36.0	36.0	36.0	32.0	36.0
21	34.30203421671207	36.0	36.0	36.0	32.0	36.0
22	34.27404773142545	36.0	36.0	36.0	32.0	36.0
23	34.217212099687366	36.0	36.0	36.0	32.0	36.0
24	34.20440583394473	36.0	36.0	36.0	32.0	36.0
25	34.17237708480342	36.0	36.0	36.0	32.0	36.0
26	34.12473538359448	36.0	36.0	36.0	32.0	36.0
27	34.13168433891462	36.0	36.0	36.0	32.0	36.0
28	34.120425816280274	36.0	36.0	36.0	32.0	36.0
29	34.079808775217415	36.0	36.0	36.0	32.0	36.0
30	34.05572903280075	36.0	36.0	36.0	32.0	36.0
31	34.072905616643844	36.0	36.0	36.0	32.0	36.0
32	34.0190019113132	36.0	36.0	36.0	32.0	36.0
33	33.98342344699961	36.0	36.0	36.0	32.0	36.0
34	33.99916444303212	36.0	36.0	36.0	32.0	36.0
35	33.94724401721584	36.0	36.0	36.0	32.0	36.0
36	33.90595591362173	36.0	36.0	36.0	32.0	36.0
37	33.9033314796555	36.0	36.0	36.0	32.0	36.0
38	33.29854712158102	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	5.0
10	7.0
11	11.0
12	5.0
13	5.0
14	163.0
15	336.0
16	408.0
17	476.0
18	600.0
19	723.0
20	1002.0
21	1375.0
22	2088.0
23	3097.0
24	4561.0
25	6672.0
26	9712.0
27	13989.0
28	19552.0
29	26802.0
30	35893.0
31	49007.0
32	66988.0
33	101006.0
34	229544.0
35	495914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73949447727843	17.08085518903765	11.192353903334594	25.98729643034933
2	17.277079072603307	19.899678218446752	36.639051992829344	26.184190716120593
3	19.0679027578368	23.664079325197417	28.389062126106722	28.878955790859056
4	12.757648972114607	15.079796058565401	35.84948337017427	36.31307159914572
5	15.026515455977988	36.15074461980182	33.17497583981188	15.647764084408314
6	34.18543400402264	35.398301219503075	16.786859873040083	13.629404903434198
7	30.44773329470206	30.38791713592755	20.79396604498362	18.370383524386767
8	28.498086812182343	31.783778933811362	19.81322352052728	19.90491073347901
9	27.777341617879063	14.053539250652369	18.629292748031673	39.53982638343689
10	16.457262596722625	25.49654607123196	30.762350447361115	27.283840884684295
11	38.13205423936654	21.04457886728403	21.850243568702076	18.97312332464736
12	25.28670528659313	23.72069156960279	27.832330448989357	23.160272694814722
13	29.465717071310323	18.695181947094238	25.263206108868197	26.57589487272724
14	24.18709731090379	19.68314858590872	24.41376693128592	31.715987171901567
15	25.601829303064328	26.664560151145604	22.07512968625695	25.65848085953312
16	26.48704227326899	25.34227560582338	23.178089874512185	24.99259224639544
17	24.55890738784553	25.390425621418487	24.919465659558952	25.131201331177028
18	25.77562219420254	23.870834474423837	25.980691346623885	24.372851984749737
19	25.945710501496926	24.60870938826404	25.128405011137	24.31717509910203
20	26.166997855838307	23.453334106004213	24.68973213868445	25.689935899473028
21	27.779320014020332	23.790723215328892	23.837551115784557	24.592405654866223
22	26.38933546693785	23.793117629904362	24.594714842260323	25.22283206089746
23	24.29339769198701	23.518233601722876	25.44983446091838	26.73853424537173
24	24.897596724590805	24.715411435889287	24.917226745436018	25.469765094083886
25	25.11916168504298	23.896650198875456	24.99698531920562	25.98720279687595
26	24.84131687827532	25.07931819074132	25.391823449018545	24.68754148196481
27	26.03185701079504	24.076056019434628	24.4180688194436	25.47401815032673
28	24.734620018488037	24.155396721691478	25.681921634275213	25.428061625545272
29	24.476602999706497	24.627747378575073	25.719221040942646	25.176428580775784
30	24.562767779032292	24.735305333522756	25.87296899184232	24.82895789560263
31	25.396096934507423	24.676388033390754	24.225962423717867	25.701552608383953
32	24.970533665462465	24.556649670752854	24.1566926668318	26.31612399695289
33	24.634248475490068	23.93125404261524	25.047670145029404	26.386827336865288
34	25.540399192006813	24.335326520291904	25.18865481846794	24.93561946923335
35	26.500226216427425	23.862000501041344	25.312870406030445	24.324902876500786
36	24.757336005503873	24.89745670186354	24.65834479973677	25.686862492895816
37	26.191629012990752	24.70196422262062	24.57923536086772	24.527171403520907
38	24.824232295644926	24.277347510650745	25.191283492136485	25.70713670156784
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	486.0
1	354.5
2	223.0
3	223.0
4	494.5
5	766.0
6	766.0
7	881.0
8	996.0
9	936.5
10	877.0
11	877.0
12	1157.5
13	1438.0
14	1813.5
15	2189.0
16	2189.0
17	3345.5
18	4502.0
19	4502.0
20	5314.0
21	6126.0
22	6519.0
23	6912.0
24	6912.0
25	8113.5
26	9315.0
27	9315.0
28	12015.0
29	14715.0
30	18165.5
31	21616.0
32	21616.0
33	28285.5
34	34955.0
35	34955.0
36	38781.0
37	42607.0
38	49449.0
39	56291.0
40	56291.0
41	59798.5
42	63306.0
43	73369.0
44	83432.0
45	83432.0
46	85445.0
47	87458.0
48	87458.0
49	92658.0
50	97858.0
51	99322.0
52	100786.0
53	100786.0
54	95419.0
55	90052.0
56	90052.0
57	87867.5
58	85683.0
59	76610.5
60	67538.0
61	67538.0
62	66562.0
63	65586.0
64	54703.5
65	43821.0
66	43821.0
67	37072.0
68	30323.0
69	30323.0
70	24570.0
71	18817.0
72	14832.5
73	10848.0
74	10848.0
75	8182.5
76	5517.0
77	5517.0
78	5421.0
79	5325.0
80	4187.5
81	3050.0
82	3050.0
83	3170.5
84	3291.0
85	3291.0
86	2194.5
87	1098.0
88	939.5
89	781.0
90	781.0
91	556.0
92	331.0
93	303.5
94	276.0
95	276.0
96	237.5
97	199.0
98	199.0
99	377.0
100	555.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1186042273200959
2	0.05476917037791662
3	0.011308992518307016
4	0.002803882442555458
5	2.803882442555458E-4
6	9.346274808518194E-5
7	0.0
8	2.803882442555458E-4
9	9.346274808518194E-5
10	3.7385099234072777E-4
11	0.001962717709788821
12	0.0028973451906406404
13	0.014580188701288384
14	0.009907051297029287
15	0.023178761525125123
16	0.011215529770221833
17	0.02009449083831412
18	0.005794690381281281
19	0.008224721831496012
20	0.005888153129366463
21	0.006542392365962737
22	0.007570482594899738
23	0.010000514045114468
24	0.014486725953203201
25	0.016823294655332752
26	0.018972937861291936
27	0.00953320030468856
28	0.005981615877451644
29	0.010000514045114468
30	0.0030842706868110044
31	0.006355466869792373
32	0.007477019846814556
33	0.008692035571921922
34	0.01252400824341438
35	0.016169055418736478
36	0.014299800457032838
37	0.010000514045114468
38	0.00626200412170719
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1069945.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.19288283290836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0566548934323	40.68467181893535
2	11.437839230079701	11.481962486740665
3	3.1529117648430125	4.74761192385791
4	1.332338628061081	2.6749566660811084
5	0.6575075037465673	1.6501098548654756
6	0.4481432354244456	1.3496160544811797
7	0.2984497398501	1.0486036976665636
8	0.22620016246237373	0.9082910601007008
9	0.1634520128644688	0.7383714957458382
>10	0.9850722956346395	9.310198981233968
>50	0.10574781405321113	3.6701125572889812
>100	0.11472207890424407	12.488693681954311
>500	0.014971886174244617	5.103690875921687
>1k	0.005988754469697846	4.143108845126296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2686	0.2510409413567987	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1955	0.1827196725065307	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1854	0.17327993494992733	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1845	0.1724387702171607	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1735	0.1621578679277907	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1629	0.15225081663076137	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1578	0.1474842164784171	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1565	0.14626920075330974	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1519	0.14196991434139136	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1517	0.14178298884522103	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1435	0.13411904350223608	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1406	0.13140862380776583	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1388	0.12972629434223254	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1380	0.12897859235755108	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1297	0.12122118426648099	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1294	0.12094079602222543	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1263	0.1180434508315848	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1253	0.11710882335073299	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1196	0.11178144670987761	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1188	0.11103374472519614	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1181	0.11037950548859989	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1166	0.10897756426732216	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1144	0.10692138380944814	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1137	0.10626714457285188	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1118	0.10449135235923342	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1110	0.10374365037455195	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	9.346274808518194E-5
9	0.0	0.0	0.0	9.346274808518194E-5	9.346274808518194E-5
10	0.0	0.0	0.0	9.346274808518194E-5	9.346274808518194E-5
11	0.0	0.0	0.0	9.346274808518194E-5	9.346274808518194E-5
12	0.0	0.0	0.0	9.346274808518194E-5	9.346274808518194E-5
13	0.0	0.0	0.0	9.346274808518194E-5	9.346274808518194E-5
14	0.0	0.0	0.0	9.346274808518194E-5	1.8692549617036389E-4
15	0.0	0.0	0.0	9.346274808518194E-5	1.8692549617036389E-4
16	0.0	0.0	0.0	9.346274808518194E-5	1.8692549617036389E-4
17	0.0	0.0	0.0	1.8692549617036389E-4	1.8692549617036389E-4
18	0.0	0.0	0.0	1.8692549617036389E-4	1.8692549617036389E-4
19	0.0	0.0	0.0	1.8692549617036389E-4	1.8692549617036389E-4
20	0.0	0.0	0.0	1.8692549617036389E-4	1.8692549617036389E-4
21	0.0	0.0	0.0	2.8038824425554587E-4	1.8692549617036389E-4
22	0.0	0.0	0.0	4.6731374042590973E-4	1.8692549617036389E-4
23	0.0	0.0	0.0	0.001495403969362911	1.8692549617036389E-4
24	0.0	0.0	0.0	0.0026169569463850946	1.8692549617036389E-4
25	0.0	0.0	0.0	0.0030842706868110044	1.8692549617036389E-4
26	0.0	0.0	0.0	0.003645047175322096	1.8692549617036389E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCG	35	0.00206996	22.850468	5
TTAGACC	40	0.0044896505	19.995092	4
TAGATCG	40	0.0044908654	19.994158	5
CAAGACG	495	0.0	18.419722	4
CTAGCGG	380	0.0	17.68483	29
TAGCGGC	385	0.0	17.455158	30
TCTAGCG	385	0.0	17.455158	28
GTTATTC	195	0.0	17.227346	3
CGCAAGA	535	0.0	17.051313	2
GGCGTTA	170	0.0	16.94177	31
AAGACGG	530	0.0	16.900723	5
GTCCTAA	95	7.620929E-7	16.856882	1
CGTTATT	190	0.0	16.846634	2
CGACGAA	95	7.6883407E-7	16.842695	32
ATAATAG	135	4.4383341E-10	16.589296	3
CGCGCCA	145	6.91216E-11	16.552305	10
GCGCAAG	570	0.0	16.014038	1
CATCGTT	240	0.0	16.000563	28
ACGCTTA	80	5.6208337E-5	15.999813	17
ATTAGCC	60	0.0024449963	15.996821	3
>>END_MODULE