FastQCFastQC Report
Thu 2 Feb 2017
SRR4063090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069945
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32010.29917425662066743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23100.2158989480767703No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19270.18010271556014562No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18970.17729883311759018No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18240.17047605250737188No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17310.16178401693544994No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17000.1588866717448093No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16420.15346583235586875No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16240.1517835028903355No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15440.14430648304352092No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15280.14281107907415802No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14730.13767062792947302No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14690.13729677693713227No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13880.12972629434223254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12790.11953885480094771No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG12520.1170153606026478No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12470.1165480468622219No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12430.11617419586988115No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT12400.11589380762562561No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT12160.11365070167158124No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC12110.11318338793115533No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT11870.11094028197711098No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11820.11047296823668505No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC11740.10972526625200361No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT11730.10963180350391842No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT11620.10860371327498142No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG11410.1066409955651926No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT11090.10365018762646679No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCA450.00884735417.7786624
TCTAGCG3800.017.68343528
ACGATTA550.001364962517.45378132
GTCGTAA957.694689E-716.84136630
CGTAAAC957.694689E-716.84136632
GTATTAA1456.91216E-1116.5525461
TAGCGGC4000.016.3992830
CTAGCGG4000.016.3992829
GAACCGC2200.016.002296
TGCGTAC908.583258E-615.999316
GGTCGTA1001.3147619E-615.99929829
TGTAGGA1501.1459633E-1015.9992982
CTAGATA3250.015.7531573
GTTATTC2950.015.7281243
GGCATCG2800.015.42789526
AGAACCG2200.015.2749145
GTCTTAA1052.1833566E-615.23885251
GTTACAC1052.1854812E-615.2374283
TATATGA951.43280195E-515.1572292
TCTATAC951.43280195E-515.1572293
Produced by FastQC (version 0.11.5)