##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063090_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1069945
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209464037871104	32.0	32.0	32.0	32.0	32.0
2	31.310978601703827	32.0	32.0	32.0	32.0	32.0
3	31.382086929701995	32.0	32.0	32.0	32.0	32.0
4	31.475491730883363	32.0	32.0	32.0	32.0	32.0
5	31.401750557271637	32.0	32.0	32.0	32.0	32.0
6	34.93571445261205	36.0	36.0	36.0	36.0	36.0
7	34.95667440849763	36.0	36.0	36.0	36.0	36.0
8	34.89752183523452	36.0	36.0	36.0	36.0	36.0
9	34.99283421110431	36.0	36.0	36.0	36.0	36.0
10	34.84552663921977	36.0	36.0	36.0	32.0	36.0
11	35.02051320394973	36.0	36.0	36.0	36.0	36.0
12	34.90641201183239	36.0	36.0	36.0	32.0	36.0
13	34.970086312847855	36.0	36.0	36.0	36.0	36.0
14	34.91210202393581	36.0	36.0	36.0	32.0	36.0
15	34.87791148143129	36.0	36.0	36.0	32.0	36.0
16	34.89017099009762	36.0	36.0	36.0	32.0	36.0
17	34.84921748314166	36.0	36.0	36.0	32.0	36.0
18	34.847712732897484	36.0	36.0	36.0	32.0	36.0
19	34.84897821850656	36.0	36.0	36.0	32.0	36.0
20	34.839075840346936	36.0	36.0	36.0	32.0	36.0
21	34.821215109187854	36.0	36.0	36.0	32.0	36.0
22	34.800650500726675	36.0	36.0	36.0	32.0	36.0
23	34.74269051212913	36.0	36.0	36.0	32.0	36.0
24	34.71144685007173	36.0	36.0	36.0	32.0	36.0
25	34.688768114248866	36.0	36.0	36.0	32.0	36.0
26	34.63134834033525	36.0	36.0	36.0	32.0	36.0
27	34.614116613470785	36.0	36.0	36.0	32.0	36.0
28	34.58407675160873	36.0	36.0	36.0	32.0	36.0
29	34.537319207996674	36.0	36.0	36.0	32.0	36.0
30	34.51730602974919	36.0	36.0	36.0	32.0	36.0
31	34.521596904513785	36.0	36.0	36.0	32.0	36.0
32	34.48326222375916	36.0	36.0	36.0	32.0	36.0
33	34.45333638644977	36.0	36.0	36.0	32.0	36.0
34	34.44544158811902	36.0	36.0	36.0	32.0	36.0
35	34.38824892868325	36.0	36.0	36.0	32.0	36.0
36	34.36886755861283	36.0	36.0	36.0	32.0	36.0
37	34.36774413638084	36.0	36.0	36.0	32.0	36.0
38	33.9127712172121	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	7.0
21	20.0
22	80.0
23	261.0
24	692.0
25	1747.0
26	3696.0
27	7272.0
28	12605.0
29	20105.0
30	30181.0
31	43380.0
32	61921.0
33	99753.0
34	244404.0
35	543819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.89985904592411	18.42684782568056	11.891270942149006	26.782022186246323
2	16.204197412016505	20.84537055643047	35.93895013295076	27.011481898602263
3	17.901387454495325	24.43742435358827	28.769703115580707	28.891485076335698
4	12.152049221313659	15.917203065957718	36.11827919805074	35.81246851467789
5	14.411021127254205	36.60094677763811	32.967395520330484	16.020636574777207
6	34.11406028171594	36.00743217162036	16.35834806153225	13.52015948513145
7	30.122960395836017	30.596352312003127	20.78522306900026	18.495464223160592
8	28.36112030177788	32.03314995616519	19.583971227916695	20.02175851414023
9	27.70233010253199	13.867615033040162	18.470712489835595	39.959342374592254
10	16.185062401220264	25.818487360399878	30.924611026318523	27.071839212061338
11	38.250279235618784	20.85121298083439	21.812531253359005	19.085976530187825
12	25.003504875983467	23.91942336845686	27.80048937415065	23.276582381409018
13	29.340320614910688	18.72079286392559	25.370580142512132	26.56830637865159
14	23.995252092397273	19.62811172536906	24.402282360308238	31.974353821925426
15	25.491403763744863	26.73829028594928	22.050105379248468	25.72020057105739
16	26.44509764520606	25.30055283215492	23.06884933337695	25.185500189262065
17	24.45695806793807	25.513180584048712	24.82557514638603	25.204286201627184
18	25.8065601502881	23.81234549439457	25.907967231960523	24.473127123356807
19	25.86768478753581	24.647622074031844	25.141479234913945	24.343213903518404
20	26.092556159428753	23.345405604961	24.77977839982429	25.782259835785954
21	27.756966719753557	23.823957141682182	23.777599575304876	24.641476563259385
22	26.215926986901195	23.796643752716264	24.79258279631196	25.19484646407058
23	24.223136911838377	23.30888345558272	25.435910664483373	27.032068968095523
24	24.811649196921337	24.654164466397805	25.06512016972835	25.46906616695251
25	25.11000575731066	23.758907008217253	25.167859252446856	25.963227982025227
26	24.946235554163998	24.99203230072574	25.37840730130988	24.683324843800385
27	25.984492617356832	23.962233466860823	24.502636591516175	25.550637324266173
28	24.56406637724369	24.019552406899418	25.944604629209913	25.47177658664698
29	24.552570459229212	24.375084700615453	25.69795643701312	25.374388403142216
30	24.7027650954021	24.464808938777228	25.924790526615855	24.90763543920482
31	25.440933879778864	24.321810934206898	24.287042791919212	25.950212394095022
32	25.097084429573485	24.353307880311608	24.32274556168775	26.226862128427165
33	24.566683334190074	23.76935263027539	25.299431279177902	26.364532756356635
34	25.428970648023963	24.115164798190563	25.523648411834255	24.932216141951223
35	26.590619143974692	23.714209608905133	25.269710125286814	24.425461121833365
36	24.7853861647094	24.75351536761235	24.82520129539369	25.635897172284555
37	26.245835066288453	24.498548990835978	24.595002546859885	24.66061339601568
38	24.887470746132507	23.99134907995185	25.325531055830957	25.795649118084686
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	270.0
1	194.5
2	119.0
3	119.0
4	428.5
5	738.0
6	738.0
7	842.0
8	946.0
9	905.0
10	864.0
11	864.0
12	1105.0
13	1346.0
14	1798.5
15	2251.0
16	2251.0
17	3519.5
18	4788.0
19	4788.0
20	5633.0
21	6478.0
22	6701.5
23	6925.0
24	6925.0
25	8106.0
26	9287.0
27	9287.0
28	11901.0
29	14515.0
30	17844.5
31	21174.0
32	21174.0
33	28307.5
34	35441.0
35	35441.0
36	39182.0
37	42923.0
38	50098.5
39	57274.0
40	57274.0
41	60453.0
42	63632.0
43	73494.0
44	83356.0
45	83356.0
46	85220.0
47	87084.0
48	87084.0
49	93142.5
50	99201.0
51	99926.0
52	100651.0
53	100651.0
54	95374.5
55	90098.0
56	90098.0
57	88012.0
58	85926.0
59	76654.0
60	67382.0
61	67382.0
62	66265.0
63	65148.0
64	54346.0
65	43544.0
66	43544.0
67	37066.5
68	30589.0
69	30589.0
70	24967.0
71	19345.0
72	15064.0
73	10783.0
74	10783.0
75	8052.0
76	5321.0
77	5321.0
78	5362.5
79	5404.0
80	4184.0
81	2964.0
82	2964.0
83	2883.5
84	2803.0
85	2803.0
86	1768.0
87	733.0
88	617.0
89	501.0
90	501.0
91	293.0
92	85.0
93	59.5
94	34.0
95	34.0
96	21.5
97	9.0
98	9.0
99	11.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008692035571921922
2	0.0
3	0.0
4	1.8692549617036389E-4
5	0.0
6	1.8692549617036389E-4
7	6.542392365962736E-4
8	0.001775792213618457
9	0.0032711961829813684
10	0.0013084784731925473
11	0.005607764885110916
12	6.542392365962736E-4
13	9.346274808518194E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	9.346274808518194E-5
22	0.0
23	9.346274808518194E-5
24	0.0
25	9.346274808518194E-5
26	0.0
27	2.803882442555458E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	9.346274808518194E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1069945.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.180957525783604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.76897480820585	38.433455871094694
2	12.206883463983619	11.7627866740077
3	3.3315669032992954	4.815542503865093
4	1.4137464514460212	2.724626309173922
5	0.7533712526367365	1.8149074162218497
6	0.47136160016678424	1.3626391942152736
7	0.3146776047118977	1.0613027814857485
8	0.23442717014760256	0.9035940422217028
9	0.1762568847793344	0.7643002931262042
>10	1.070338562850076	9.605004016414172
>50	0.1112464818244577	3.7509882323250956
>100	0.12333959120441082	12.757450239620285
>500	0.01697870944855424	5.606372614595655
>1k	0.006830515295395384	4.637029811632643
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3201	0.29917425662066743	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2310	0.2158989480767703	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1927	0.18010271556014562	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1897	0.17729883311759018	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1824	0.17047605250737188	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1731	0.16178401693544994	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1700	0.1588866717448093	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1642	0.15346583235586875	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1624	0.1517835028903355	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1544	0.14430648304352092	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1528	0.14281107907415802	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1473	0.13767062792947302	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1469	0.13729677693713227	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1388	0.12972629434223254	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1279	0.11953885480094771	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1252	0.1170153606026478	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1247	0.1165480468622219	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1243	0.11617419586988115	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1240	0.11589380762562561	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1216	0.11365070167158124	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1211	0.11318338793115533	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1187	0.11094028197711098	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1182	0.11047296823668505	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1174	0.10972526625200361	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1173	0.10963180350391842	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1162	0.10860371327498142	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1141	0.1066409955651926	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1109	0.10365018762646679	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.346274808518194E-5
2	0.0	0.0	0.0	0.0	9.346274808518194E-5
3	0.0	0.0	0.0	0.0	9.346274808518194E-5
4	0.0	0.0	0.0	0.0	9.346274808518194E-5
5	0.0	0.0	0.0	0.0	1.8692549617036389E-4
6	0.0	0.0	0.0	0.0	2.8038824425554587E-4
7	0.0	0.0	0.0	0.0	2.8038824425554587E-4
8	0.0	0.0	0.0	0.0	2.8038824425554587E-4
9	0.0	0.0	0.0	9.346274808518194E-5	2.8038824425554587E-4
10	0.0	0.0	0.0	9.346274808518194E-5	2.8038824425554587E-4
11	0.0	0.0	0.0	9.346274808518194E-5	2.8038824425554587E-4
12	0.0	0.0	0.0	1.8692549617036389E-4	2.8038824425554587E-4
13	0.0	0.0	0.0	1.8692549617036389E-4	2.8038824425554587E-4
14	0.0	9.346274808518194E-5	0.0	1.8692549617036389E-4	2.8038824425554587E-4
15	0.0	9.346274808518194E-5	0.0	1.8692549617036389E-4	2.8038824425554587E-4
16	0.0	9.346274808518194E-5	0.0	1.8692549617036389E-4	2.8038824425554587E-4
17	0.0	9.346274808518194E-5	0.0	1.8692549617036389E-4	2.8038824425554587E-4
18	0.0	9.346274808518194E-5	0.0	1.8692549617036389E-4	4.6731374042590973E-4
19	0.0	9.346274808518194E-5	0.0	2.8038824425554587E-4	4.6731374042590973E-4
20	0.0	9.346274808518194E-5	0.0	2.8038824425554587E-4	4.6731374042590973E-4
21	0.0	9.346274808518194E-5	0.0	4.6731374042590973E-4	4.6731374042590973E-4
22	0.0	9.346274808518194E-5	0.0	6.542392365962736E-4	4.6731374042590973E-4
23	0.0	9.346274808518194E-5	0.0	0.001495403969362911	4.6731374042590973E-4
24	0.0	9.346274808518194E-5	0.0	0.0024300314502147305	4.6731374042590973E-4
25	0.0	9.346274808518194E-5	0.0	0.0028973451906406404	4.6731374042590973E-4
26	0.0	9.346274808518194E-5	0.0	0.0033646589310665502	4.6731374042590973E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCA	45	0.008847354	17.778662	4
TCTAGCG	380	0.0	17.683435	28
ACGATTA	55	0.0013649625	17.453781	32
GTCGTAA	95	7.694689E-7	16.841366	30
CGTAAAC	95	7.694689E-7	16.841366	32
GTATTAA	145	6.91216E-11	16.552546	1
TAGCGGC	400	0.0	16.39928	30
CTAGCGG	400	0.0	16.39928	29
GAACCGC	220	0.0	16.00229	6
TGCGTAC	90	8.583258E-6	15.9993	16
GGTCGTA	100	1.3147619E-6	15.999298	29
TGTAGGA	150	1.1459633E-10	15.999298	2
CTAGATA	325	0.0	15.753157	3
GTTATTC	295	0.0	15.728124	3
GGCATCG	280	0.0	15.427895	26
AGAACCG	220	0.0	15.274914	5
GTCTTAA	105	2.1833566E-6	15.2388525	1
GTTACAC	105	2.1854812E-6	15.237428	3
TATATGA	95	1.43280195E-5	15.157229	2
TCTATAC	95	1.43280195E-5	15.157229	3
>>END_MODULE