FastQCFastQC Report
Thu 2 Feb 2017
SRR4063089_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063089_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136156
Sequences flagged as poor quality0
Sequence length38
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT50193.686212873468668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31932.34510414524516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18891.3873791827021946No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15771.158230265283939No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA14411.058344839742648No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10380.7623608214107348No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8360.6140015864155821No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8250.6059226181732719No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA6660.4891448044889685No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA5720.4201063486001352No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.41569963864978404No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5610.4120273803578249No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA4750.3488645377361262No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4490.32976879461793823No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG4000.29378066335673786No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA3390.24897911219483532No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3100.22768001410147184No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.20784981932489202No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA2820.20711536766650018No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2690.1975674961074062No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA2640.19389523781544699No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA2630.19316078615705515No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA2610.19169188284027144No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2600.1909574311818796No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACT2540.18655072123152852No Hit
GTCCTTGACAGTCCTTGAAAATCCGACGGAACTTATAA2070.15203149328711185No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2050.15056258997032815No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2030.14909368665354447No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA1990.14615588001997706No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG1970.1446869767031934No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA1960.14395252504480155No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA1940.14248362172801785No Hit
TTCTTGGACTGGTCCGGACCTGCGAGAGGACTCTTTTA1900.13954581509445046No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC1860.1366080084608831No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT1830.13440465348570757No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1830.13440465348570757No Hit
GTTCTAAGTCGGTTGTTAAGCGTACGCCAGACGCCCGA1790.1314668468521402No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1740.12779458856018097No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA1670.12265342695143805No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1640.12045007197626252No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA1610.11824671700108699No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1590.11677781368430329No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACAATGCG1580.11604336202591145No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA1550.11384000705073592No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1500.11016774875877669No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1490.10943329710038485No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA1410.10355768383325009No Hit
GTCCAAGAAGGTTGCCCCTCCCAGTCCGACCCAGTCCC1400.10282323217485824No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCG1370.10061987719968271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGAA200.003743892331.99669312
GGGGGTA555.789673E-520.3615327
TGGGATC400.004466228719.99793219
TGGGGGT802.7623628E-617.998146
AGTACGA450.00881663217.77594225
TTTAGCA600.002428771715.99834724
GTTAAGA600.002428771715.99834711
ATGGGAT703.669477E-415.99834618
TCCAAGA756.130434E-414.9537582
AGTCCGA650.00413942614.76770523
TTAGCAG650.00413942614.76770525
CCCAACC650.00413942614.7677057
TGTCTCT650.00413942614.76770532
AGGCTGC650.00413942614.7677056
ATGGGGA800.001010662413.9985535
CGTACCC800.001010662413.9985533
GGCTGCT800.001010662413.9985537
GACGTAC700.00668600713.7380941
ACGTACC700.00670232813.7330432
CTGTCTC700.006767946813.71286931
Produced by FastQC (version 0.11.5)