##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063089_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136156
Sequences flagged as poor quality	0
Sequence length	38
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.214716942330856	32.0	32.0	32.0	21.0	32.0
2	30.216038955315963	32.0	32.0	32.0	21.0	32.0
3	30.395737242574693	32.0	32.0	32.0	21.0	32.0
4	30.249948588383912	32.0	32.0	32.0	21.0	32.0
5	30.482094068568408	32.0	32.0	32.0	32.0	32.0
6	33.95599165662916	36.0	36.0	36.0	32.0	36.0
7	33.52838655659684	36.0	36.0	36.0	21.0	36.0
8	33.39619994711948	36.0	36.0	36.0	21.0	36.0
9	33.29839301977144	36.0	36.0	36.0	21.0	36.0
10	33.36680719175064	36.0	36.0	36.0	21.0	36.0
11	33.76757542818532	36.0	36.0	36.0	32.0	36.0
12	33.67840565233996	36.0	36.0	36.0	27.0	36.0
13	33.70018948852786	36.0	36.0	36.0	27.0	36.0
14	33.63223067657687	36.0	36.0	36.0	27.0	36.0
15	33.706806897969976	36.0	36.0	36.0	32.0	36.0
16	33.63441199800229	36.0	36.0	36.0	27.0	36.0
17	33.56744469579012	36.0	36.0	36.0	27.0	36.0
18	33.65289080172743	36.0	36.0	36.0	27.0	36.0
19	33.50891624313288	36.0	36.0	36.0	21.0	36.0
20	33.308264050060224	36.0	36.0	36.0	21.0	36.0
21	33.23970298774935	36.0	36.0	36.0	21.0	36.0
22	33.299164194012754	36.0	36.0	36.0	21.0	36.0
23	33.365301565850935	36.0	36.0	36.0	21.0	36.0
24	33.388657128587795	36.0	36.0	36.0	21.0	36.0
25	33.36517670906901	36.0	36.0	36.0	21.0	36.0
26	33.35288933282411	36.0	36.0	36.0	21.0	36.0
27	33.429940656306	36.0	36.0	36.0	21.0	36.0
28	33.37423984253356	36.0	36.0	36.0	21.0	36.0
29	33.29275977555157	36.0	36.0	36.0	21.0	36.0
30	33.22102588207644	36.0	36.0	36.0	21.0	36.0
31	33.21494462234496	36.0	36.0	36.0	21.0	36.0
32	33.08784041834367	36.0	36.0	36.0	21.0	36.0
33	32.858390375745465	36.0	36.0	36.0	14.0	36.0
34	32.725719028173565	36.0	36.0	36.0	14.0	36.0
35	32.59989277005788	36.0	36.0	36.0	14.0	36.0
36	32.492875818913596	36.0	36.0	36.0	14.0	36.0
37	32.46160286729928	36.0	36.0	36.0	14.0	36.0
38	31.596389435647346	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	0.0
11	2.0
12	3.0
13	1.0
14	69.0
15	129.0
16	182.0
17	257.0
18	329.0
19	512.0
20	600.0
21	724.0
22	939.0
23	1181.0
24	1358.0
25	1696.0
26	2264.0
27	2925.0
28	3777.0
29	4908.0
30	6343.0
31	8019.0
32	10141.0
33	13941.0
34	26810.0
35	49044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.92218536958456	16.257896556035213	12.862469388205358	17.957448686174867
2	15.040787829793489	21.25450135959433	40.335121628573525	23.369589182038656
3	19.827383575730863	31.652710445130012	26.687968267959455	21.83193771117967
4	11.005185304875361	15.386988263290096	33.54216548907855	40.065660942756
5	11.56108846535199	40.75208402188682	29.03822848958907	18.648599023172117
6	28.770886122433993	35.288458007418015	19.303000257059967	16.637655613088025
7	27.745380299068717	34.55154381738594	19.812567936778404	17.89050794676694
8	32.71492049502405	26.59248650435166	18.857919283169917	21.834673717454372
9	31.39927729956814	12.236699080466524	19.76042186903258	36.603601750932754
10	19.187549575486944	26.914715473427535	26.669408619524663	27.228326331560858
11	35.45570057215045	24.795634323151162	19.26656041365229	20.482104691046104
12	28.03778130324931	26.44764674775251	27.667606792408485	17.846965156589693
13	31.43171967971792	22.022331594799088	27.3055167854257	19.2404319400573
14	27.009695901278096	18.63375936535919	28.850448068165125	25.506096665197592
15	26.132665280604183	23.500371004356545	30.83082936003585	19.536134355003416
16	22.066169124992655	27.480607627666455	27.581242287124642	22.871980960216256
17	19.703792948920444	24.62588432350629	29.082213618765657	26.588109108807608
18	21.144834070308942	23.488710317168838	34.226028705322385	21.140426907199835
19	22.266864496415558	24.420466564813726	34.48848278293571	18.824186155834997
20	25.336408508637913	19.914943001527792	34.65007638970502	20.098572100129275
21	26.413944059231405	20.2506169937713	32.13582677165354	21.199612175343756
22	24.390351392643083	21.707897520272653	32.90045833823011	21.001292748854155
23	21.321541896815138	23.506728170172757	33.701081208132564	21.470648724879542
24	21.071305267642675	25.8541279484034	33.416585251188174	19.65798153276575
25	22.861571462153268	23.86944640338505	32.571550305594734	20.69743182886695
26	20.891827364554636	23.846464646464646	33.92249770431589	21.33921028466483
27	20.40252681063611	26.13045394446893	33.810048479506385	19.65697076538857
28	20.398104961621797	24.849976128392523	34.49924712622571	20.25267178375996
29	18.863489451724746	26.819504025386376	34.80563554092966	19.511370981959217
30	20.199631291727567	26.489706281995723	33.774265337749995	19.53639708852671
31	19.306479808440937	27.38383793868347	31.251744450810158	22.05793780206543
32	18.67287577858738	27.36367375719826	32.317252321071805	21.646198143142556
33	19.586598990737407	26.962148980086532	32.183283261960206	21.267968767215862
34	19.485069967311862	27.62919161126823	32.82036214052228	20.065376280897638
35	20.24520498637342	27.469129000741933	32.39085880512154	19.894807207763108
36	19.773745684272388	29.2404319400573	31.332549768603542	19.653272607066775
37	19.987512395783597	29.939398391302753	30.54467991332134	19.528409299592315
38	19.655815729940944	31.40626377177777	30.04965184945794	18.88826864882334
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75.0
1	103.5
2	132.0
3	132.0
4	467.0
5	802.0
6	802.0
7	1074.5
8	1347.0
9	1412.5
10	1478.0
11	1478.0
12	1854.5
13	2231.0
14	2604.5
15	2978.0
16	2978.0
17	4120.5
18	5263.0
19	5263.0
20	5755.0
21	6247.0
22	4590.5
23	2934.0
24	2934.0
25	2140.5
26	1347.0
27	1347.0
28	1995.0
29	2643.0
30	3300.0
31	3957.0
32	3957.0
33	3398.0
34	2839.0
35	2839.0
36	3353.0
37	3867.0
38	4712.5
39	5558.0
40	5558.0
41	6495.0
42	7432.0
43	8577.0
44	9722.0
45	9722.0
46	12155.5
47	14589.0
48	14589.0
49	13225.0
50	11861.0
51	10560.5
52	9260.0
53	9260.0
54	8361.5
55	7463.0
56	7463.0
57	7383.5
58	7304.0
59	6776.5
60	6249.0
61	6249.0
62	5725.5
63	5202.0
64	4804.0
65	4406.0
66	4406.0
67	3809.0
68	3212.0
69	3212.0
70	2730.0
71	2248.0
72	1836.0
73	1424.0
74	1424.0
75	1131.0
76	838.0
77	838.0
78	697.5
79	557.0
80	428.5
81	300.0
82	300.0
83	242.5
84	185.0
85	185.0
86	123.0
87	61.0
88	49.0
89	37.0
90	37.0
91	25.0
92	13.0
93	14.5
94	16.0
95	16.0
96	12.0
97	8.0
98	8.0
99	39.5
100	71.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1314668468521402
2	0.06316284262169863
3	0.011751226534269514
4	0.0014689033167836893
5	7.344516583918446E-4
6	7.344516583918446E-4
7	0.0
8	7.344516583918446E-4
9	0.0
10	0.0
11	0.002203354975175534
12	0.0029378066335673785
13	0.01909574311818796
14	0.011751226534269514
15	0.028643614677281944
16	0.014689033167836893
17	0.02570580804371456
18	0.010282323217485826
19	0.008813419900702136
20	0.008813419900702136
21	0.008813419900702136
22	0.008813419900702136
23	0.008813419900702136
24	0.01689238814301243
25	0.02056464643497165
26	0.022768001410147187
27	0.011751226534269514
28	0.00807896824231029
29	0.014689033167836893
30	0.003672258291959223
31	0.007344516583918446
32	0.008813419900702136
33	0.01248567819266136
34	0.015423484826228738
35	0.018361291459796116
36	0.01909574311818796
37	0.015423484826228738
38	0.005875613267134757
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	136156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.8573694879403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.75152269303818	33.98014042715708
2	13.237605606130067	11.876083316196127
3	4.756369113890891	6.400746202884926
4	2.0777392101643857	3.728076617997003
5	1.0609732137009629	2.3796233731895766
6	0.640185997773266	1.7230235905872675
7	0.39950225947999213	1.2544434325332707
8	0.31599973803130527	1.1339933605570083
9	0.20630034710852055	0.8328681806163519
>10	1.3016569519942367	10.917623901994771
>50	0.12607243434409587	3.9542877287816913
>100	0.1064247822385225	8.46455536296601
>500	0.009823826052786692	2.9569023766855667
>1k	0.008186521710655576	6.711419254384676
>5k	0.0016373043421311153	3.686212873468668
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5019	3.686212873468668	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3193	2.34510414524516	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1889	1.3873791827021946	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1577	1.158230265283939	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1441	1.058344839742648	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1038	0.7623608214107348	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	836	0.6140015864155821	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	825	0.6059226181732719	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	666	0.4891448044889685	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	572	0.4201063486001352	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	566	0.41569963864978404	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	561	0.4120273803578249	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	475	0.3488645377361262	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	449	0.32976879461793823	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	400	0.29378066335673786	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	339	0.24897911219483532	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	310	0.22768001410147184	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	283	0.20784981932489202	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	282	0.20711536766650018	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	269	0.1975674961074062	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	264	0.19389523781544699	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	263	0.19316078615705515	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	261	0.19169188284027144	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	260	0.1909574311818796	No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACT	254	0.18655072123152852	No Hit
GTCCTTGACAGTCCTTGAAAATCCGACGGAACTTATAA	207	0.15203149328711185	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	205	0.15056258997032815	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	203	0.14909368665354447	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	199	0.14615588001997706	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	197	0.1446869767031934	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	196	0.14395252504480155	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	194	0.14248362172801785	No Hit
TTCTTGGACTGGTCCGGACCTGCGAGAGGACTCTTTTA	190	0.13954581509445046	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	186	0.1366080084608831	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	183	0.13440465348570757	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	183	0.13440465348570757	No Hit
GTTCTAAGTCGGTTGTTAAGCGTACGCCAGACGCCCGA	179	0.1314668468521402	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	174	0.12779458856018097	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	167	0.12265342695143805	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	164	0.12045007197626252	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	161	0.11824671700108699	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	159	0.11677781368430329	No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACAATGCG	158	0.11604336202591145	No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA	155	0.11384000705073592	No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	150	0.11016774875877669	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	149	0.10943329710038485	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	141	0.10355768383325009	No Hit
GTCCAAGAAGGTTGCCCCTCCCAGTCCGACCCAGTCCC	140	0.10282323217485824	No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCG	137	0.10061987719968271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	7.344516583918446E-4	0.0
19	0.0	0.0	0.0	0.0014689033167836893	7.344516583918446E-4
20	0.0	0.0	0.0	0.0014689033167836893	7.344516583918446E-4
21	0.0	0.0	0.0	0.002203354975175534	7.344516583918446E-4
22	0.0	0.0	7.344516583918446E-4	0.004406709950351068	7.344516583918446E-4
23	0.0	0.0	7.344516583918446E-4	0.011016774875877669	7.344516583918446E-4
24	0.0	0.0	7.344516583918446E-4	0.022768001410147183	7.344516583918446E-4
25	0.0	0.0	7.344516583918446E-4	0.027174711360498253	7.344516583918446E-4
26	0.0	0.0	7.344516583918446E-4	0.0345192279444167	7.344516583918446E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGAA	20	0.0037438923	31.996693	12
GGGGGTA	55	5.789673E-5	20.361532	7
TGGGATC	40	0.0044662287	19.997932	19
TGGGGGT	80	2.7623628E-6	17.99814	6
AGTACGA	45	0.008816632	17.775942	25
TTTAGCA	60	0.0024287717	15.998347	24
GTTAAGA	60	0.0024287717	15.998347	11
ATGGGAT	70	3.669477E-4	15.998346	18
TCCAAGA	75	6.130434E-4	14.953758	2
AGTCCGA	65	0.004139426	14.767705	23
TTAGCAG	65	0.004139426	14.767705	25
CCCAACC	65	0.004139426	14.767705	7
TGTCTCT	65	0.004139426	14.767705	32
AGGCTGC	65	0.004139426	14.767705	6
ATGGGGA	80	0.0010106624	13.998553	5
CGTACCC	80	0.0010106624	13.998553	3
GGCTGCT	80	0.0010106624	13.998553	7
GACGTAC	70	0.006686007	13.738094	1
ACGTACC	70	0.006702328	13.733043	2
CTGTCTC	70	0.0067679468	13.712869	31
>>END_MODULE