FastQCFastQC Report
Thu 2 Feb 2017
SRR4063089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136156
Sequences flagged as poor quality0
Sequence length38
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46443.410793501571727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31472.311319368959135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20851.5313317077469961No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA16721.2280031728311642No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15441.1339933605570083No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10300.7564852081436No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9980.7329827550750609No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA9340.6859778489379829No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8780.6448485560680396No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA7240.5317430006756955No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA6880.5053027409735891No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA6650.4884103528305767No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5690.4179029936249596No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.4032139604571227No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG4970.3650224742207468No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA4680.3437233761273833No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.3136108581333177No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3660.26880930697141514No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA3260.23943124063574134No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA3210.23575898234378212No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA2780.20417756103293283No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2760.20270865771614913No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA2750.20197420605775726No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACT2680.1968330444490144No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA2670.1960985927906225No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA2580.1894885278650959No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2560.18801962454831223No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA2560.18801962454831223No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC2550.1872851728899204No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA2520.18508181791474485No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.17994065630600192No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG2300.16892388143012427No Hit
TTCTTGGACTGGTCCGGACCTGCGAGAGGACTCTTTTA1990.14615588001997706No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC1970.1446869767031934No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA1970.1446869767031934No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1950.1432180733864097No Hit
GTCCTTGACAGTCCTTGAAAATCCGACGGAACTTATAA1880.1380769117776668No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1880.1380769117776668No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA1800.13220129851053206No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1750.1285290402185728No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACAATGCG1750.1285290402185728No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1740.12779458856018097No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1720.12632568524339727No Hit
GTTCTAAGTCGGTTGTTAAGCGTACGCCAGACGCCCGA1700.12485678192661359No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1700.12485678192661359No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA1590.11677781368430329No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTT1580.11604336202591145No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT1550.11384000705073592No Hit
GTCCAAGAAGGTTGCCCCTCCCAGTCCGACCCAGTCCC1510.11090220041716854No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT1470.10796439378360116No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1440.10576103880842562No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1440.10576103880842562No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1430.1050265871500338No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1410.10355768383325009No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1400.10282323217485824No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAG1370.10061987719968271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCC200.003743260431.9977998
AGGTGCC200.003743260431.9977997
CGCCCAA200.003743260431.99779913
AACTTAT453.815676E-728.44248630
GGAACTT356.392761E-527.42668528
GAACTTA401.584254E-423.9983529
ACCGGAC350.002058079722.8555712
GACGGAA502.7757658E-522.39845825
TCCGACG604.6730784E-621.33186522
CGACGGA555.7881378E-520.36223224
ACGGAAC555.7881378E-520.36223226
CCGACGG555.7881378E-520.36223223
ACGCACC400.00446533819.9986258
CACCGGA400.00446533819.99862511
CGGAACT507.1547757E-419.19867927
ATCCGAC601.1294467E-418.66538221
GAAAATC701.7695505E-518.28445617
TGCCCCT802.761455E-617.99876213
CGCACCG450.00881488317.7765549
TGGTCCG450.00881488317.77655410
Produced by FastQC (version 0.11.5)