##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063089_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136156
Sequences flagged as poor quality	0
Sequence length	38
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.139538470577868	32.0	32.0	32.0	21.0	32.0
2	31.22582185140574	32.0	32.0	32.0	32.0	32.0
3	31.323452510355768	32.0	32.0	32.0	32.0	32.0
4	31.468991450982696	32.0	32.0	32.0	32.0	32.0
5	31.230404829754107	32.0	32.0	32.0	32.0	32.0
6	34.6927274596786	36.0	36.0	36.0	32.0	36.0
7	34.791114603836775	36.0	36.0	36.0	32.0	36.0
8	34.78882311466259	36.0	36.0	36.0	32.0	36.0
9	34.83938276682629	36.0	36.0	36.0	32.0	36.0
10	34.70866506066571	36.0	36.0	36.0	32.0	36.0
11	34.896266047768734	36.0	36.0	36.0	36.0	36.0
12	34.80459179176827	36.0	36.0	36.0	32.0	36.0
13	34.812119921266785	36.0	36.0	36.0	32.0	36.0
14	34.72063662269749	36.0	36.0	36.0	32.0	36.0
15	34.70969329298745	36.0	36.0	36.0	32.0	36.0
16	34.691890184788036	36.0	36.0	36.0	32.0	36.0
17	34.654513939892475	36.0	36.0	36.0	32.0	36.0
18	34.60764123505391	36.0	36.0	36.0	32.0	36.0
19	34.52802667528423	36.0	36.0	36.0	32.0	36.0
20	34.46997561620494	36.0	36.0	36.0	32.0	36.0
21	34.40052586738741	36.0	36.0	36.0	32.0	36.0
22	34.438827521372545	36.0	36.0	36.0	32.0	36.0
23	34.40658509356914	36.0	36.0	36.0	32.0	36.0
24	34.37104497781956	36.0	36.0	36.0	32.0	36.0
25	34.36374452833515	36.0	36.0	36.0	32.0	36.0
26	34.32741854931108	36.0	36.0	36.0	32.0	36.0
27	34.32296042774465	36.0	36.0	36.0	32.0	36.0
28	34.223016246070685	36.0	36.0	36.0	32.0	36.0
29	34.16223302682217	36.0	36.0	36.0	32.0	36.0
30	34.11221686888569	36.0	36.0	36.0	32.0	36.0
31	34.061569082522986	36.0	36.0	36.0	32.0	36.0
32	33.95331825259262	36.0	36.0	36.0	32.0	36.0
33	33.75894562119921	36.0	36.0	36.0	32.0	36.0
34	33.63624812714827	36.0	36.0	36.0	27.0	36.0
35	33.503312376979345	36.0	36.0	36.0	21.0	36.0
36	33.491142512999794	36.0	36.0	36.0	21.0	36.0
37	33.40311848174153	36.0	36.0	36.0	21.0	36.0
38	32.99786274567408	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	2.0
22	14.0
23	48.0
24	115.0
25	288.0
26	681.0
27	1333.0
28	2409.0
29	3687.0
30	5648.0
31	7569.0
32	10585.0
33	15572.0
34	31852.0
35	56350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.05802850007346	17.803731452916118	13.680035257822828	18.4582047891876
2	14.88292840565234	22.175298921824965	38.82458356590969	24.117189106613
3	19.582093937057028	31.364253975248797	27.531122617604936	21.522529470089236
4	10.907341578777284	16.180704486030727	32.71468021974794	40.19727371544405
5	11.58670936278974	41.30629571961574	28.737624489556097	18.369370428038426
6	29.415955462197218	35.24758729086182	18.517267212127443	16.81919003481352
7	27.839390111342873	34.63013014483386	19.20297306031317	18.32750668351009
8	33.260374586852734	26.746235769372017	18.554535438854206	21.438854204921043
9	31.03070900263682	12.284335544146487	19.24802973213171	37.43692572108499
10	19.736919415964042	27.486853315314786	26.065689356326565	26.710537912394607
11	35.904159596894445	24.256847579383443	18.80081972631718	21.038173097404936
12	27.594083257439994	27.383295631481534	27.02194541555275	18.000675695525718
13	32.27474367637122	21.403390228855137	27.006521930726517	19.315344164047122
14	26.753870560239722	19.100884279796702	28.437968212932223	25.70727694703135
15	26.76341843179882	24.018038132730105	29.405241047034288	19.813302388436792
16	22.49258203825024	27.245218719703868	27.116689679485294	23.145509562560594
17	19.869855166132965	25.40688621874908	28.557683833250096	26.165574781867857
18	21.736096830106643	23.189576662064106	33.55268956197303	21.521636945856223
19	23.12421046446723	24.607068362760362	33.22806192896384	19.040659243808573
20	26.36167337465848	19.87646523105849	33.386703487176476	20.37515790710655
21	27.26431446282206	20.339169775845352	30.77646229325186	21.62005346808073
22	24.903052381092277	22.290607832192485	31.60933047386821	21.19700931284703
23	21.847733482182203	23.480419518787272	33.00258526983754	21.669261729192986
24	21.426158230265287	26.28602485384412	32.160169217662094	20.1276476982285
25	23.361438350127795	24.433003319721497	31.340521166896796	20.865037163253913
26	20.98989394518053	24.63277417080408	32.81970680689797	21.55762507711742
27	20.92232439260848	26.272070272334673	32.957783718675635	19.847821616381207
28	20.701988895090924	24.820059343694	33.64229266429683	20.835659096918242
29	19.074444020094596	27.00358412409295	33.66065395575663	20.26131790005582
30	20.536002820294367	26.734040365463148	32.460559945944354	20.269396868298127
31	19.38585152325274	27.82690443315021	29.887775786597725	22.899468256999324
32	18.79241458327213	27.82690443315021	31.46317459384823	21.91750638972943
33	19.874261876083317	27.267986721114017	31.205382061752694	21.652369341049972
34	19.55771321131643	27.732894620876053	32.01180998266694	20.697582185140572
35	20.527189400393667	28.01859631599048	31.129733540938336	20.324480742677515
36	19.88087194100884	29.62704544786862	30.388671817620967	20.10341079350157
37	20.241487705279237	30.349011428067808	29.89658920649843	19.51291166015453
38	19.977085108258173	31.39560504127618	29.477951761215078	19.149358089250565
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	54.0
1	59.5
2	65.0
3	65.0
4	275.5
5	486.0
6	486.0
7	791.5
8	1097.0
9	1251.5
10	1406.0
11	1406.0
12	1754.0
13	2102.0
14	2451.5
15	2801.0
16	2801.0
17	3875.5
18	4950.0
19	4950.0
20	5344.5
21	5739.0
22	4351.0
23	2963.0
24	2963.0
25	2069.5
26	1176.0
27	1176.0
28	1822.0
29	2468.0
30	3314.0
31	4160.0
32	4160.0
33	3460.5
34	2761.0
35	2761.0
36	3186.0
37	3611.0
38	4450.5
39	5290.0
40	5290.0
41	6421.5
42	7553.0
43	8585.0
44	9617.0
45	9617.0
46	12637.0
47	15657.0
48	15657.0
49	14394.5
50	13132.0
51	11381.0
52	9630.0
53	9630.0
54	8636.0
55	7642.0
56	7642.0
57	7468.0
58	7294.0
59	6702.0
60	6110.0
61	6110.0
62	5663.0
63	5216.0
64	4798.5
65	4381.0
66	4381.0
67	3753.5
68	3126.0
69	3126.0
70	2707.0
71	2288.0
72	1888.5
73	1489.0
74	1489.0
75	1162.5
76	836.0
77	836.0
78	677.5
79	519.0
80	405.5
81	292.0
82	292.0
83	222.5
84	153.0
85	153.0
86	99.5
87	46.0
88	34.5
89	23.0
90	23.0
91	15.5
92	8.0
93	8.0
94	8.0
95	8.0
96	7.0
97	6.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011751226534269514
2	0.0
3	7.344516583918446E-4
4	0.0
5	0.0
6	0.0014689033167836893
7	0.0
8	0.004406709950351068
9	0.005141161608742913
10	0.0
11	0.00954787155909398
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	136156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.36945856223743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.44080103680295	30.38206175269544
2	14.181476023931685	11.73359969446811
3	5.299412359969464	6.577014600898969
4	2.3558861647167433	3.8984694027439115
5	1.230315834324569	2.5448749963277417
6	0.75274735029382	1.8684450189488526
7	0.44383688106946934	1.285290402185728
8	0.3160118593214622	1.0458591615499868
9	0.2769542137873489	1.03117012838215
>10	1.3954231540824116	10.733276535738417
>50	0.15978127718500895	4.768794617938248
>100	0.12249897917517355	9.348835159669791
>500	0.014202780194223018	4.4103822086430275
>1k	0.010652085145667263	10.37192631980963
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4644	3.410793501571727	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3147	2.311319368959135	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2085	1.5313317077469961	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1672	1.2280031728311642	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1544	1.1339933605570083	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1030	0.7564852081436	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	998	0.7329827550750609	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	934	0.6859778489379829	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	878	0.6448485560680396	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	724	0.5317430006756955	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	688	0.5053027409735891	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	665	0.4884103528305767	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	569	0.4179029936249596	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	549	0.4032139604571227	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	497	0.3650224742207468	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	468	0.3437233761273833	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	427	0.3136108581333177	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	366	0.26880930697141514	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	326	0.23943124063574134	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	321	0.23575898234378212	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	278	0.20417756103293283	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	276	0.20270865771614913	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	275	0.20197420605775726	No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACT	268	0.1968330444490144	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	267	0.1960985927906225	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	258	0.1894885278650959	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	256	0.18801962454831223	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	256	0.18801962454831223	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	255	0.1872851728899204	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	252	0.18508181791474485	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	245	0.17994065630600192	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	230	0.16892388143012427	No Hit
TTCTTGGACTGGTCCGGACCTGCGAGAGGACTCTTTTA	199	0.14615588001997706	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	197	0.1446869767031934	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	197	0.1446869767031934	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	195	0.1432180733864097	No Hit
GTCCTTGACAGTCCTTGAAAATCCGACGGAACTTATAA	188	0.1380769117776668	No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	188	0.1380769117776668	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	180	0.13220129851053206	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	175	0.1285290402185728	No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACAATGCG	175	0.1285290402185728	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	174	0.12779458856018097	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	172	0.12632568524339727	No Hit
GTTCTAAGTCGGTTGTTAAGCGTACGCCAGACGCCCGA	170	0.12485678192661359	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	170	0.12485678192661359	No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA	159	0.11677781368430329	No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTT	158	0.11604336202591145	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	155	0.11384000705073592	No Hit
GTCCAAGAAGGTTGCCCCTCCCAGTCCGACCCAGTCCC	151	0.11090220041716854	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	147	0.10796439378360116	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	144	0.10576103880842562	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	144	0.10576103880842562	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA	143	0.1050265871500338	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	141	0.10355768383325009	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	140	0.10282323217485824	No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAG	137	0.10061987719968271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	7.344516583918446E-4	0.0
19	0.0	0.0	0.0	0.0014689033167836893	0.0
20	0.0	0.0	0.0	0.0014689033167836893	0.0
21	0.0	0.0	0.0	0.002203354975175534	0.0
22	0.0	0.0	0.0	0.004406709950351068	0.0
23	0.0	0.0	0.0	0.011016774875877669	0.0
24	0.0	0.0	0.0	0.022768001410147183	0.0
25	0.0	0.0	0.0	0.027174711360498253	0.0
26	0.0	0.0	0.0	0.03378477628602485	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGTCC	20	0.0037432604	31.997799	8
AGGTGCC	20	0.0037432604	31.997799	7
CGCCCAA	20	0.0037432604	31.997799	13
AACTTAT	45	3.815676E-7	28.442486	30
GGAACTT	35	6.392761E-5	27.426685	28
GAACTTA	40	1.584254E-4	23.99835	29
ACCGGAC	35	0.0020580797	22.85557	12
GACGGAA	50	2.7757658E-5	22.398458	25
TCCGACG	60	4.6730784E-6	21.331865	22
CGACGGA	55	5.7881378E-5	20.362232	24
ACGGAAC	55	5.7881378E-5	20.362232	26
CCGACGG	55	5.7881378E-5	20.362232	23
ACGCACC	40	0.004465338	19.998625	8
CACCGGA	40	0.004465338	19.998625	11
CGGAACT	50	7.1547757E-4	19.198679	27
ATCCGAC	60	1.1294467E-4	18.665382	21
GAAAATC	70	1.7695505E-5	18.284456	17
TGCCCCT	80	2.761455E-6	17.998762	13
CGCACCG	45	0.008814883	17.776554	9
TGGTCCG	45	0.008814883	17.776554	10
>>END_MODULE