FastQCFastQC Report
Thu 2 Feb 2017
SRR4063088_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063088_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745265
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27590.3702038872079059No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23420.3142506356799259No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19290.2588341059891448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17830.23924375893138683No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG10560.14169456502049607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9800.13149685011371795No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9700.13015504552072082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT400.004488230719.9952186
GGTCGTA507.194515E-419.19927429
GTCGTAA507.197765E-419.19798730
ACCGTTC507.197765E-419.1979878
TTCGGAC1401.8189894E-1218.2813434
TTAGAGC450.0088596317.7735274
CGCTTCG652.1008376E-417.22896232
GTTATTC652.1029935E-417.226653
GTATAGA854.9271293E-616.9598851
CGGATTA1354.4201443E-1016.59085318
TCGGACG1354.4383341E-1016.5886275
CGGACGT1354.4383341E-1016.5886276
CGCGGTT908.564844E-616.00154310
CGTTTAG600.002441438815.99939629
CGTAAAC600.002442532715.99832332
CTTCGGA1501.1459633E-1015.9961763
TGGCGTA1551.8735591E-1015.48536414
CTAGCGG1255.1304596E-815.3594229
TAGCGGC1255.1346433E-815.35838930
GACGGAC1052.1847045E-615.2364977
Produced by FastQC (version 0.11.5)