##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063088_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	745265
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15724473844874	32.0	32.0	32.0	32.0	32.0
2	30.785728566348883	32.0	32.0	32.0	32.0	32.0
3	30.842456039127022	32.0	32.0	32.0	32.0	32.0
4	30.902301865779286	32.0	32.0	32.0	32.0	32.0
5	30.828357698268402	32.0	32.0	32.0	32.0	32.0
6	34.44318061360724	36.0	36.0	36.0	32.0	36.0
7	34.38236063682046	36.0	36.0	36.0	32.0	36.0
8	34.34348855776133	36.0	36.0	36.0	32.0	36.0
9	34.497649829255366	36.0	36.0	36.0	32.0	36.0
10	34.2265945670332	36.0	36.0	36.0	32.0	36.0
11	34.48909985038879	36.0	36.0	36.0	32.0	36.0
12	34.32573916660517	36.0	36.0	36.0	32.0	36.0
13	34.38274170932487	36.0	36.0	36.0	32.0	36.0
14	34.29724997148665	36.0	36.0	36.0	32.0	36.0
15	34.26238854635599	36.0	36.0	36.0	32.0	36.0
16	34.26180888677182	36.0	36.0	36.0	32.0	36.0
17	34.206091792852206	36.0	36.0	36.0	32.0	36.0
18	34.22632486431001	36.0	36.0	36.0	32.0	36.0
19	34.206691579505275	36.0	36.0	36.0	32.0	36.0
20	34.18148443842123	36.0	36.0	36.0	32.0	36.0
21	34.16395107780454	36.0	36.0	36.0	32.0	36.0
22	34.14516715530717	36.0	36.0	36.0	32.0	36.0
23	34.08604187772135	36.0	36.0	36.0	32.0	36.0
24	34.08541793858561	36.0	36.0	36.0	32.0	36.0
25	34.03852723527873	36.0	36.0	36.0	32.0	36.0
26	34.00315994981651	36.0	36.0	36.0	32.0	36.0
27	33.98679664280491	36.0	36.0	36.0	32.0	36.0
28	33.967111027621044	36.0	36.0	36.0	32.0	36.0
29	33.92938350787975	36.0	36.0	36.0	32.0	36.0
30	33.898033585368964	36.0	36.0	36.0	32.0	36.0
31	33.9111336236104	36.0	36.0	36.0	32.0	36.0
32	33.85979215446854	36.0	36.0	36.0	32.0	36.0
33	33.819150235151255	36.0	36.0	36.0	32.0	36.0
34	33.80886932835971	36.0	36.0	36.0	32.0	36.0
35	33.76598793717671	36.0	36.0	36.0	32.0	36.0
36	33.706352773845545	36.0	36.0	36.0	27.0	36.0
37	33.68708982710848	36.0	36.0	36.0	27.0	36.0
38	33.06342978672016	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	1.0
10	8.0
11	6.0
12	1.0
13	2.0
14	57.0
15	151.0
16	249.0
17	309.0
18	386.0
19	510.0
20	746.0
21	1075.0
22	1545.0
23	2285.0
24	3564.0
25	5205.0
26	7597.0
27	11092.0
28	15584.0
29	21257.0
30	28202.0
31	37926.0
32	51270.0
33	74878.0
34	159945.0
35	321409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.12495214041498	17.46911797303742	11.423428022542705	24.98250186400489
2	16.475133849218924	19.59608950847667	37.6992970566597	26.229479585644704
3	19.48462905208462	23.93836590746834	28.234555243621607	28.34244979682543
4	12.437016511570176	15.116708152461975	36.577992176964315	35.86828315900353
5	13.825283623945845	37.311426137011665	34.08800896325468	14.775281275787808
6	33.38190698548157	36.182540321498536	16.96924563239675	13.466307060623139
7	29.796649513931285	30.920679221484974	21.046607582537753	18.236063682045984
8	28.3422854543576	32.763555318203956	19.598637255941206	19.295521971497234
9	27.01418557719144	14.67614160887954	19.132012280212113	39.17766053371691
10	15.570384066790025	27.223616961037813	32.069436076757	25.136562895415164
11	36.865396666080734	22.266249267023504	21.512675629219228	19.355678437676534
12	24.30978888765141	24.20016021619794	29.49709021084398	21.992960685306674
13	29.15563989285465	20.382980208171176	25.54659695419483	24.914782944779347
14	23.224721649290856	20.105261321563674	25.901880452446548	30.76813657669892
15	25.09495599142108	27.285318931339102	23.394874658091325	24.224850419148495
16	25.552480997981707	26.5793414780779	24.078885214926782	23.789292309013614
17	23.47428362830136	26.13724971782652	26.19764356624333	24.19082308762879
18	24.347847090270843	25.54319238242461	26.8171717497021	23.29178877760244
19	25.00043611575183	26.051273793468997	25.911985131807597	23.03630495897157
20	24.731589689501504	24.878391779082985	26.66202374865979	23.727994782755722
21	25.689023792941963	24.823508730967056	25.462111239484663	24.02535623660632
22	24.142530487804876	25.204907033665407	26.504261851597388	24.148300626932325
23	23.68360666077115	24.995336816071948	26.30330958576166	25.017746937395245
24	23.6283239282575	26.418712634114595	26.014238457254436	23.938724980373475
25	24.863414012225974	25.124172129882506	25.983076892106798	24.029336965784722
26	23.496483787846252	25.38329396607258	26.465804165771956	24.654418080309213
27	24.211458591931077	25.98332002603311	25.895961513945824	23.90925986808999
28	23.430033694612217	25.372539494889452	26.63752997424932	24.559896836249013
29	23.58923915333549	26.291796780119782	26.6915643539909	23.42739971255383
30	24.587011683500194	25.751336148537586	26.7319611560855	22.929691011876727
31	24.27239704731885	25.369252879347197	25.582612406252643	24.77573766708131
32	23.579440619309544	25.645550222284246	26.081530614572667	24.693478543833542
33	23.266917515203893	25.35600989796083	26.688262542874163	24.68881004396112
34	23.541742152797347	26.079820712052282	27.01625132520096	23.362185809949406
35	24.57202673323134	25.56808116595539	26.33639316101672	23.523498939796546
36	24.136986999950345	25.982116376412296	25.84509716822496	24.035799455412395
37	24.85849398414918	25.68766958580136	25.503154866223472	23.95068156382599
38	23.942836056224632	26.24536213895132	25.766043812271462	24.045757992552584
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	72.0
2	98.0
3	98.0
4	337.0
5	576.0
6	576.0
7	718.0
8	860.0
9	878.0
10	896.0
11	896.0
12	1172.5
13	1449.0
14	1945.5
15	2442.0
16	2442.0
17	3343.5
18	4245.0
19	4245.0
20	5123.5
21	6002.0
22	6336.0
23	6670.0
24	6670.0
25	7868.5
26	9067.0
27	9067.0
28	11372.5
29	13678.0
30	16163.5
31	18649.0
32	18649.0
33	22447.5
34	26246.0
35	26246.0
36	30345.0
37	34444.0
38	39999.5
39	45555.0
40	45555.0
41	48395.0
42	51235.0
43	56272.5
44	61310.0
45	61310.0
46	68399.0
47	75488.0
48	75488.0
49	74946.0
50	74404.0
51	69458.0
52	64512.0
53	64512.0
54	60956.5
55	57401.0
56	57401.0
57	54990.5
58	52580.0
59	47105.5
60	41631.0
61	41631.0
62	39079.0
63	36527.0
64	29999.5
65	23472.0
66	23472.0
67	19188.5
68	14905.0
69	14905.0
70	11810.0
71	8715.0
72	6659.5
73	4604.0
74	4604.0
75	3526.0
76	2448.0
77	2448.0
78	2163.5
79	1879.0
80	1493.5
81	1108.0
82	1108.0
83	1042.0
84	976.0
85	976.0
86	634.0
87	292.0
88	270.5
89	249.0
90	249.0
91	180.5
92	112.0
93	96.5
94	81.0
95	81.0
96	73.0
97	65.0
98	65.0
99	206.5
100	348.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12076241336974097
2	0.05326964234198574
3	0.012478782714873233
4	0.004025413778991366
5	0.0
6	6.709022964985609E-4
7	0.0
8	5.367218371988488E-4
9	1.341804592997122E-4
10	5.367218371988488E-4
11	0.002146887348795395
12	0.0029519701045936683
13	0.014625670063668628
14	0.009660993069579276
15	0.024555024051847327
16	0.010868617203276686
17	0.0209321516507551
18	0.0055013988312882
19	0.006709022964985609
20	0.005903940209187337
21	0.005903940209187337
22	0.006574842505685897
23	0.008855910313781004
24	0.013418045929971218
25	0.017443459708962585
26	0.019456166598458266
27	0.008050827557982731
28	0.005635579290587911
29	0.0110027976625764
30	0.0034886919417925167
31	0.005903940209187337
32	0.006440662046386184
33	0.008185008017282443
34	0.012747143633472659
35	0.016772557412464025
36	0.015296572360167187
37	0.008990090773080717
38	0.005367218371988487
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	745265.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.212799925380345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.36198197404106	48.176707601612954
2	11.998553011100594	14.209358376807385
3	3.2107037410201142	5.7034427471008255
4	1.2247407607515353	2.900813185073551
5	0.5758501974960091	1.7048851265660974
6	0.3195740264750652	1.1353723734609742
7	0.20632652522861428	0.8552019880362616
8	0.13832985950864826	0.6552718635833248
9	0.09699580745503382	0.516905400639207
>10	0.6720296549306508	7.9353188161557915
>50	0.10780592545525222	4.481501291991617
>100	0.08255976893802036	9.1042339717967
>500	0.0034115606994973356	1.2919088869635231
>1k	0.0011371868998324452	1.3290783702119262
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2759	0.3702038872079059	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2342	0.3142506356799259	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1929	0.2588341059891448	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1783	0.23924375893138683	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1056	0.14169456502049607	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	980	0.13149685011371795	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	970	0.13015504552072082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.683609185994244E-4
2	0.0	0.0	0.0	0.0	2.683609185994244E-4
3	0.0	0.0	0.0	0.0	2.683609185994244E-4
4	0.0	0.0	0.0	0.0	2.683609185994244E-4
5	0.0	0.0	0.0	0.0	2.683609185994244E-4
6	0.0	0.0	0.0	0.0	2.683609185994244E-4
7	0.0	0.0	0.0	0.0	2.683609185994244E-4
8	0.0	0.0	0.0	0.0	2.683609185994244E-4
9	0.0	0.0	0.0	0.0	2.683609185994244E-4
10	0.0	0.0	0.0	0.0	2.683609185994244E-4
11	0.0	0.0	0.0	0.0	2.683609185994244E-4
12	0.0	0.0	0.0	0.0	2.683609185994244E-4
13	0.0	0.0	0.0	0.0	2.683609185994244E-4
14	0.0	1.341804592997122E-4	0.0	0.0	4.0254137789913654E-4
15	0.0	1.341804592997122E-4	0.0	0.0	6.70902296498561E-4
16	0.0	1.341804592997122E-4	0.0	0.0	6.70902296498561E-4
17	0.0	1.341804592997122E-4	0.0	0.0	6.70902296498561E-4
18	0.0	1.341804592997122E-4	0.0	1.341804592997122E-4	8.050827557982731E-4
19	0.0	1.341804592997122E-4	0.0	1.341804592997122E-4	8.050827557982731E-4
20	0.0	1.341804592997122E-4	0.0	2.683609185994244E-4	8.050827557982731E-4
21	0.0	1.341804592997122E-4	0.0	4.0254137789913654E-4	8.050827557982731E-4
22	0.0	1.341804592997122E-4	0.0	4.0254137789913654E-4	8.050827557982731E-4
23	0.0	1.341804592997122E-4	0.0	0.0012076241336974097	8.050827557982731E-4
24	0.0	1.341804592997122E-4	0.0	0.0034886919417925167	8.050827557982731E-4
25	0.0	2.683609185994244E-4	0.0	0.00429377469759079	8.050827557982731E-4
26	0.0	2.683609185994244E-4	0.0	0.0052330379126887755	9.392632150979853E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACCGT	40	0.0044882307	19.995218	6
GGTCGTA	50	7.194515E-4	19.199274	29
GTCGTAA	50	7.197765E-4	19.197987	30
ACCGTTC	50	7.197765E-4	19.197987	8
TTCGGAC	140	1.8189894E-12	18.281343	4
TTAGAGC	45	0.00885963	17.773527	4
CGCTTCG	65	2.1008376E-4	17.228962	32
GTTATTC	65	2.1029935E-4	17.22665	3
GTATAGA	85	4.9271293E-6	16.959885	1
CGGATTA	135	4.4201443E-10	16.590853	18
TCGGACG	135	4.4383341E-10	16.588627	5
CGGACGT	135	4.4383341E-10	16.588627	6
CGCGGTT	90	8.564844E-6	16.001543	10
CGTTTAG	60	0.0024414388	15.999396	29
CGTAAAC	60	0.0024425327	15.998323	32
CTTCGGA	150	1.1459633E-10	15.996176	3
TGGCGTA	155	1.8735591E-10	15.485364	14
CTAGCGG	125	5.1304596E-8	15.35942	29
TAGCGGC	125	5.1346433E-8	15.358389	30
GACGGAC	105	2.1847045E-6	15.236497	7
>>END_MODULE