FastQCFastQC Report
Thu 2 Feb 2017
SRR4063088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745265
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA24130.3237774482902055No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC23100.30995686098233516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23050.3092859586858366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15060.20207577170536653No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG12310.1651761453979457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9470.12706889495682744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7880.1057342019281732No Hit
TCCGAAGACTGGCCAAGGGCGCCTGCTCGGGTCCAAAA7870.1056000214688735No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7500.10063534447478414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT400.0044808320.0009234
ATACAAC1003.255991E-919.2008863
CGCGTAA601.1376832E-418.66627510
AGCGTAT953.7820428E-818.527178
GTATTAG1550.017.5515521
GACAATC550.001363613917.455357
GGACGCA1101.0608346E-817.455356
GCGTAAC652.0994921E-417.23040811
AATAGCG854.9770024E-616.9419575
ATAGCGT957.679755E-716.8428826
CGTATAT957.6851575E-716.84175110
ACGCACC1151.8366336E-816.6964238
GTACCGG600.002440073316.0007386
TAGCGTA908.568946E-616.0007387
GACGCAC1203.103014E-816.0007387
TCGCGTA703.6929216E-416.0007389
TCTATAC805.6143912E-516.0007383
CGCACCG1304.78758E-916.0007369
CCGAAAA703.6948087E-415.99966422
TACGACG1052.1812775E-615.2387985
Produced by FastQC (version 0.11.5)