##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063088_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	745265
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.106835823499022	32.0	32.0	32.0	32.0	32.0
2	31.193892105492676	32.0	32.0	32.0	32.0	32.0
3	31.312511656927402	32.0	32.0	32.0	32.0	32.0
4	31.42396463003093	32.0	32.0	32.0	32.0	32.0
5	31.328908509053825	32.0	32.0	32.0	32.0	32.0
6	34.83530824606012	36.0	36.0	36.0	32.0	36.0
7	34.87621181727305	36.0	36.0	36.0	32.0	36.0
8	34.80356785841278	36.0	36.0	36.0	32.0	36.0
9	34.93774563410331	36.0	36.0	36.0	32.0	36.0
10	34.75906020006307	36.0	36.0	36.0	32.0	36.0
11	34.96092530844733	36.0	36.0	36.0	36.0	36.0
12	34.86281658202116	36.0	36.0	36.0	32.0	36.0
13	34.909801211649544	36.0	36.0	36.0	32.0	36.0
14	34.861403661784735	36.0	36.0	36.0	32.0	36.0
15	34.82318906697618	36.0	36.0	36.0	32.0	36.0
16	34.83141298732666	36.0	36.0	36.0	32.0	36.0
17	34.804089820399454	36.0	36.0	36.0	32.0	36.0
18	34.804164961456664	36.0	36.0	36.0	32.0	36.0
19	34.7877949454221	36.0	36.0	36.0	32.0	36.0
20	34.7844605610085	36.0	36.0	36.0	32.0	36.0
21	34.7668131470014	36.0	36.0	36.0	32.0	36.0
22	34.73966844008507	36.0	36.0	36.0	32.0	36.0
23	34.68024259827041	36.0	36.0	36.0	32.0	36.0
24	34.66380817561539	36.0	36.0	36.0	32.0	36.0
25	34.642578143344984	36.0	36.0	36.0	32.0	36.0
26	34.57431115106707	36.0	36.0	36.0	32.0	36.0
27	34.55402574923014	36.0	36.0	36.0	32.0	36.0
28	34.516268709787795	36.0	36.0	36.0	32.0	36.0
29	34.48232105358497	36.0	36.0	36.0	32.0	36.0
30	34.46630929937673	36.0	36.0	36.0	32.0	36.0
31	34.4585174401052	36.0	36.0	36.0	32.0	36.0
32	34.41858131000382	36.0	36.0	36.0	32.0	36.0
33	34.37340140755302	36.0	36.0	36.0	32.0	36.0
34	34.35977404010654	36.0	36.0	36.0	32.0	36.0
35	34.321936492388616	36.0	36.0	36.0	32.0	36.0
36	34.28580035289461	36.0	36.0	36.0	32.0	36.0
37	34.26710498950038	36.0	36.0	36.0	32.0	36.0
38	33.82253426633479	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	17.0
22	32.0
23	173.0
24	461.0
25	1166.0
26	2472.0
27	4984.0
28	9083.0
29	14993.0
30	22479.0
31	33138.0
32	47320.0
33	74775.0
34	175051.0
35	359119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.447635432085015	18.811335110958428	12.162283662265546	25.57874579469101
2	15.738428612641142	20.695994042387607	36.77792463083601	26.78765271413524
3	18.439883799722246	24.879204041515436	28.607810644535835	28.073101514226483
4	12.082816179479783	16.156132382441147	36.53572890180003	35.225322536279045
5	13.463264744755222	37.79836702381032	33.66171764405949	15.07665058737496
6	33.54542819725627	36.60407587110071	16.568222804268018	13.282273127374996
7	29.80955665196488	31.08910837948048	20.708181429056395	18.393153539498243
8	28.35683107256769	33.29088667009281	19.11106220873829	19.241220048601207
9	26.909426128869075	14.512296088358468	18.864736989709314	39.713540793063146
10	15.357593323135548	27.477122078200892	32.17011512760647	24.99516947105708
11	37.05993333583816	22.22111890589189	21.335215531354162	19.38373222691579
12	24.275554839921636	24.35485602340123	29.356868743794113	22.01272039288302
13	29.406760557332706	20.41075377315958	25.365642242212154	24.816843427295563
14	23.285006004575553	20.101842968608484	25.685360240988103	30.92779078582786
15	25.148370042870656	27.434268347500552	23.038516500841983	24.378845108786805
16	25.70887631765388	26.70946134524142	23.801766890658882	23.779895446445824
17	23.582618263302315	26.295344608964598	25.98297249971487	24.13906462801822
18	24.614331814857803	25.709110182284157	26.402957337322963	23.273600665535078
19	24.940792872334004	26.469242484217027	25.58995793442601	23.000006709022966
20	25.078059482197606	24.8959765989279	26.247978906831797	23.777985012042695
21	26.035069451898924	24.969540994868932	24.9306286094592	24.064760943772946
22	24.296592487236087	25.47429437850966	25.952916076831734	24.27619705742253
23	23.699891984730264	25.20660436220673	25.907160540210526	25.186343112852477
24	23.730753490369196	26.681650151288466	25.59036047580391	23.997235882538426
25	24.938679447819833	25.211200326327315	25.6774780480474	24.172642177805447
26	23.656283335457857	25.458595264771596	26.128692478514353	24.756428921256195
27	24.38676175588549	26.08884088210234	25.54218969091531	23.98220767109686
28	23.734644723688888	25.489859311788425	26.102258928032313	24.673237036490374
29	23.673324253788923	26.310238639946864	26.375316162707225	23.641120943556988
30	24.626944778031977	25.857379589810336	26.492724064594476	23.022951567563215
31	24.405278659268852	25.506497688741586	25.27168188496709	24.816541767022468
32	23.74376899492127	25.823163572688912	25.67435744332553	24.758709989064293
33	23.444009848845713	25.537761735758423	26.244087673512105	24.77414074188376
34	23.718811429491524	26.169349157682163	26.67225751913749	23.43958189368882
35	24.772262215453562	25.660805216936254	25.95385534004683	23.613077227563352
36	24.235808739173315	26.075557016631667	25.663891367500153	24.024742876694866
37	25.078864564953406	25.75258465109726	25.15481070491704	24.013740079032292
38	24.095053437367916	26.189878767955022	25.522330982938957	24.192736811738104
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	50.0
2	69.0
3	69.0
4	210.0
5	351.0
6	351.0
7	494.5
8	638.0
9	707.0
10	776.0
11	776.0
12	984.0
13	1192.0
14	1673.0
15	2154.0
16	2154.0
17	3025.0
18	3896.0
19	3896.0
20	4705.0
21	5514.0
22	6069.0
23	6624.0
24	6624.0
25	7721.5
26	8819.0
27	8819.0
28	11427.5
29	14036.0
30	16571.0
31	19106.0
32	19106.0
33	22955.0
34	26804.0
35	26804.0
36	30573.0
37	34342.0
38	39743.5
39	45145.0
40	45145.0
41	47905.0
42	50665.0
43	55517.0
44	60369.0
45	60369.0
46	68531.5
47	76694.0
48	76694.0
49	76136.0
50	75578.0
51	70092.0
52	64606.0
53	64606.0
54	61215.5
55	57825.0
56	57825.0
57	55145.0
58	52465.0
59	47420.5
60	42376.0
61	42376.0
62	39707.0
63	37038.0
64	30281.0
65	23524.0
66	23524.0
67	19233.5
68	14943.0
69	14943.0
70	11870.0
71	8797.0
72	6634.0
73	4471.0
74	4471.0
75	3410.5
76	2350.0
77	2350.0
78	2074.0
79	1798.0
80	1409.5
81	1021.0
82	1021.0
83	917.5
84	814.0
85	814.0
86	519.0
87	224.0
88	184.0
89	144.0
90	144.0
91	90.0
92	36.0
93	25.0
94	14.0
95	14.0
96	11.5
97	9.0
98	9.0
99	8.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010197714906778127
2	0.0
3	0.0
4	0.0
5	0.0
6	1.341804592997122E-4
7	5.367218371988488E-4
8	0.002146887348795395
9	0.003757052860391941
10	6.709022964985609E-4
11	0.004964676994089351
12	6.709022964985609E-4
13	1.341804592997122E-4
14	0.0
15	0.0
16	1.341804592997122E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.341804592997122E-4
22	0.0
23	0.0
24	0.0
25	1.341804592997122E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	745265.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.33610055547197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.77767664743594	45.74140890339314
2	13.033822763856959	14.946171452214042
3	3.5642866727280538	6.130868972281927
4	1.2929989998630684	2.965420826970943
5	0.6052770117250478	1.735211180409146
6	0.3524383795241788	1.2124465420803543
7	0.1988082821130846	0.797922415814752
8	0.15188067426614504	0.6966596489725261
9	0.1061406433784123	0.5477121541590424
>10	0.7099907760141754	8.161409147762267
>50	0.11273429532993826	4.606221828497705
>100	0.08854302467384628	9.587848215938259
>500	0.004227518419137675	1.5557361016056035
>1k	0.0011743106719826874	1.3149626099003477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2413	0.3237774482902055	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2310	0.30995686098233516	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2305	0.3092859586858366	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1506	0.20207577170536653	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1231	0.1651761453979457	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	947	0.12706889495682744	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	788	0.1057342019281732	No Hit
TCCGAAGACTGGCCAAGGGCGCCTGCTCGGGTCCAAAA	787	0.1056000214688735	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	750	0.10063534447478414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.683609185994244E-4
13	0.0	0.0	0.0	0.0	2.683609185994244E-4
14	0.0	0.0	0.0	0.0	6.70902296498561E-4
15	0.0	0.0	0.0	0.0	8.050827557982731E-4
16	0.0	0.0	0.0	0.0	8.050827557982731E-4
17	0.0	0.0	0.0	0.0	8.050827557982731E-4
18	0.0	0.0	0.0	0.0	8.050827557982731E-4
19	0.0	0.0	0.0	0.0	8.050827557982731E-4
20	0.0	0.0	0.0	1.341804592997122E-4	0.0010734436743976975
21	0.0	0.0	0.0	2.683609185994244E-4	0.0010734436743976975
22	0.0	0.0	0.0	2.683609185994244E-4	0.0010734436743976975
23	0.0	0.0	0.0	0.0010734436743976975	0.0010734436743976975
24	0.0	0.0	0.0	0.0032203310231930923	0.0010734436743976975
25	0.0	0.0	0.0	0.004025413778991366	0.0010734436743976975
26	0.0	0.0	0.0	0.005098857453389063	0.0010734436743976975
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACT	40	0.00448083	20.000923	4
ATACAAC	100	3.255991E-9	19.200886	3
CGCGTAA	60	1.1376832E-4	18.666275	10
AGCGTAT	95	3.7820428E-8	18.52717	8
GTATTAG	155	0.0	17.551552	1
GACAATC	55	0.0013636139	17.45535	7
GGACGCA	110	1.0608346E-8	17.45535	6
GCGTAAC	65	2.0994921E-4	17.230408	11
AATAGCG	85	4.9770024E-6	16.941957	5
ATAGCGT	95	7.679755E-7	16.842882	6
CGTATAT	95	7.6851575E-7	16.841751	10
ACGCACC	115	1.8366336E-8	16.696423	8
GTACCGG	60	0.0024400733	16.000738	6
TAGCGTA	90	8.568946E-6	16.000738	7
GACGCAC	120	3.103014E-8	16.000738	7
TCGCGTA	70	3.6929216E-4	16.000738	9
TCTATAC	80	5.6143912E-5	16.000738	3
CGCACCG	130	4.78758E-9	16.000736	9
CCGAAAA	70	3.6948087E-4	15.999664	22
TACGACG	105	2.1812775E-6	15.238798	5
>>END_MODULE