FastQCFastQC Report
Thu 2 Feb 2017
SRR4063087_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063087_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458243
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41900.2873320838845103No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26190.17959969634690515No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17830.12227043092269257No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17470.1198017065742815No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17410.119390252516213No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17150.11760728493124945No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16180.11095544432580852No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15900.10903532538815547No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15760.10807526591932895No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15610.10704663077415767No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15510.10636087401071015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14680.10066909287409574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT1354.3655746E-1016.6104321
TCTAGCG3900.016.40930628
GTATTAG2550.016.3312631
CATCGTT3150.016.25302728
ATCGTTT3250.015.75293429
AACGGAC650.004161994414.76938915
TAGCCGA650.00416484214.7678719
CTAGCGG4300.014.51078729
TACTATA1002.3172839E-514.4060832
TAGCGGC4350.014.34399730
GCATCGT3600.014.22139927
CGTCGTA3600.013.777925510
CGCTTCG3750.013.65254332
GTAACAC1301.3389254E-613.536753
GTCTTAC952.4056747E-413.488171
GTATCAA37800.013.3900411
TACCGTC3350.013.3714437
CGGCATC4000.013.19968925
TTTCGTT5000.013.11924228
ACCGTCG3550.013.0687698
Produced by FastQC (version 0.11.5)