##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063087_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1458243
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.221172328617385	32.0	32.0	32.0	32.0	32.0
2	30.885504679261274	32.0	32.0	32.0	32.0	32.0
3	30.929581695231864	32.0	32.0	32.0	32.0	32.0
4	30.98547567175018	32.0	32.0	32.0	32.0	32.0
5	30.931777488388423	32.0	32.0	32.0	32.0	32.0
6	34.56949493328615	36.0	36.0	36.0	32.0	36.0
7	34.49405003144195	36.0	36.0	36.0	32.0	36.0
8	34.48578323365859	36.0	36.0	36.0	32.0	36.0
9	34.582841131416366	36.0	36.0	36.0	32.0	36.0
10	34.35620332139431	36.0	36.0	36.0	32.0	36.0
11	34.5868610375637	36.0	36.0	36.0	32.0	36.0
12	34.415468478161735	36.0	36.0	36.0	32.0	36.0
13	34.49448960152732	36.0	36.0	36.0	32.0	36.0
14	34.402179197842884	36.0	36.0	36.0	32.0	36.0
15	34.351794591162104	36.0	36.0	36.0	32.0	36.0
16	34.36905440314131	36.0	36.0	36.0	32.0	36.0
17	34.305403831871644	36.0	36.0	36.0	32.0	36.0
18	34.32047127947811	36.0	36.0	36.0	32.0	36.0
19	34.307624312271685	36.0	36.0	36.0	32.0	36.0
20	34.28208261586032	36.0	36.0	36.0	32.0	36.0
21	34.268043803399024	36.0	36.0	36.0	32.0	36.0
22	34.25516186259766	36.0	36.0	36.0	32.0	36.0
23	34.19963682321808	36.0	36.0	36.0	32.0	36.0
24	34.19220870595642	36.0	36.0	36.0	32.0	36.0
25	34.175340461089135	36.0	36.0	36.0	32.0	36.0
26	34.12063490104187	36.0	36.0	36.0	32.0	36.0
27	34.12868637120151	36.0	36.0	36.0	32.0	36.0
28	34.11715948576472	36.0	36.0	36.0	32.0	36.0
29	34.089329419033724	36.0	36.0	36.0	32.0	36.0
30	34.059752729826236	36.0	36.0	36.0	32.0	36.0
31	34.07792871284141	36.0	36.0	36.0	32.0	36.0
32	34.037547240069046	36.0	36.0	36.0	32.0	36.0
33	33.99278926763235	36.0	36.0	36.0	32.0	36.0
34	33.9938727633186	36.0	36.0	36.0	32.0	36.0
35	33.95156568555446	36.0	36.0	36.0	32.0	36.0
36	33.918108298822624	36.0	36.0	36.0	32.0	36.0
37	33.91602291250498	36.0	36.0	36.0	32.0	36.0
38	33.318606021081536	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	7.0
10	9.0
11	12.0
12	6.0
13	4.0
14	209.0
15	384.0
16	497.0
17	615.0
18	800.0
19	975.0
20	1375.0
21	1900.0
22	2923.0
23	4311.0
24	6426.0
25	9650.0
26	13875.0
27	20099.0
28	27866.0
29	37374.0
30	50268.0
31	66911.0
32	91012.0
33	135395.0
34	305024.0
35	680313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.61051537359814	17.211962171240092	11.295622668144224	25.88189978701754
2	16.734550764176408	20.080678694594294	37.19090206712566	25.99386847410364
3	18.86655930910747	24.5002935415375	28.454736992269158	28.178410157085874
4	12.748853712763667	15.523396878038879	35.99005888137727	35.737690527820185
5	14.72047487349485	36.33055167150012	33.45880413457035	15.490169320434688
6	33.60690531524668	35.53032116134598	17.168927727210697	13.693845796196646
7	29.620577640352124	30.70825644285623	21.40706315751216	18.26410275927949
8	27.905664233136157	32.42694265270827	19.959224763035937	19.70816835111964
9	27.830927809600748	14.205676565121953	18.648289274543785	39.31510635073352
10	16.090709410460153	26.346367090716953	31.397116659203327	26.16580683961957
11	37.18095454928862	21.351966133984174	22.39790099827392	19.06917831845329
12	25.18011808998704	23.821466043985435	28.375542617318494	22.622873248709034
13	29.412769272869806	19.43037501534587	25.25940521807596	25.897450493708362
14	24.026294222335	19.70262976514198	25.066952880945603	31.204123131577415
15	25.098051320040334	26.589089861514086	22.789678375208346	25.523180443237237
16	25.777109326134905	25.631645652961122	23.586581404790905	25.004663616113064
17	24.124754880610823	25.546121540807277	25.170254663527125	25.15886891505477
18	25.14622770580405	24.539440161682	26.0534601223039	24.26087201021005
19	25.643112947847328	24.93735178055361	25.50931253609045	23.91022273550861
20	25.888107993932064	23.927618169798016	24.97764299567399	25.206630840595928
21	27.22469032848292	24.0672821463792	24.359362977125937	24.348664548011946
22	26.00882498978145	24.073746409790058	24.901997898681326	25.015430701747164
23	24.365669876065844	24.017411544921416	25.521821696946674	26.095096882066066
24	24.88117756632452	24.95545444694244	25.16203059137565	25.001337395357385
25	24.95349845269188	24.17970721857648	25.314948532800734	25.551845795930912
26	24.73982785736675	25.129545939339238	25.69738754452231	24.433238658771707
27	25.5136443752529	24.724952164102845	24.650472186597717	25.11093127404654
28	24.56308069599853	24.45019901739448	25.727215868722585	25.25950441788441
29	24.641312070843266	24.82579974377474	25.573009110536233	24.959879074845755
30	24.57339732973439	25.013526886100536	25.7289945803305	24.68408120383457
31	25.012755766841043	24.885540995388723	24.650725158486974	25.450978079283264
32	24.805301504918553	24.87429294065365	24.550938448117893	25.769467106309907
33	24.3271324474925	24.436930990141448	25.31525075010716	25.920685812258892
34	25.192395664659205	24.54043480974416	25.45603310693708	24.811136418659547
35	25.9652282577161	24.310247506383647	25.36708954522665	24.357434690673603
36	24.72317156058882	25.176933581064354	24.831673905871813	25.268220952475012
37	25.582315628341245	24.986197801112542	24.792658396091905	24.638828174454307
38	24.583688232171735	24.84998237507218	25.359390794712795	25.206938598043287
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	506.0
1	408.0
2	310.0
3	310.0
4	720.0
5	1130.0
6	1130.0
7	1267.0
8	1404.0
9	1382.0
10	1360.0
11	1360.0
12	1767.0
13	2174.0
14	2841.0
15	3508.0
16	3508.0
17	5087.0
18	6666.0
19	6666.0
20	8137.0
21	9608.0
22	10139.5
23	10671.0
24	10671.0
25	12061.5
26	13452.0
27	13452.0
28	17425.5
29	21399.0
30	26118.5
31	30838.0
32	30838.0
33	39627.5
34	48417.0
35	48417.0
36	54636.5
37	60856.0
38	69400.5
39	77945.0
40	77945.0
41	84335.0
42	90725.0
43	102958.0
44	115191.0
45	115191.0
46	119664.5
47	124138.0
48	124138.0
49	130231.5
50	136325.0
51	136951.0
52	137577.0
53	137577.0
54	131674.0
55	125771.0
56	125771.0
57	121772.0
58	117773.0
59	105384.0
60	92995.0
61	92995.0
62	87882.5
63	82770.0
64	67880.5
65	52991.0
66	52991.0
67	44363.0
68	35735.0
69	35735.0
70	28572.0
71	21409.0
72	16814.5
73	12220.0
74	12220.0
75	9347.0
76	6474.0
77	6474.0
78	6096.5
79	5719.0
80	4514.0
81	3309.0
82	3309.0
83	3358.0
84	3407.0
85	3407.0
86	2289.0
87	1171.0
88	987.5
89	804.0
90	804.0
91	587.5
92	371.0
93	300.5
94	230.0
95	230.0
96	219.5
97	209.0
98	209.0
99	447.0
100	685.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12240758227538208
2	0.05876935462745235
3	0.0128236514764686
4	0.003908813551650857
5	1.3715135268950373E-4
6	2.7430270537900746E-4
7	0.0
8	3.4287838172375934E-4
9	1.3715135268950373E-4
10	2.7430270537900746E-4
11	0.0017829675849635484
12	0.0022629973193768116
13	0.013440832563571366
14	0.008777686572128239
15	0.02283570022280237
16	0.010080624422678526
17	0.019201189376530526
18	0.004937448696822134
19	0.006926143310819938
20	0.005966083841993413
21	0.005828932489303909
22	0.007611900074267458
23	0.008914837924817743
24	0.012686500123779096
25	0.015566678530258674
26	0.018241129907703997
27	0.009120564953851999
28	0.005417478431235397
29	0.010354927128057531
30	0.002468724348411067
31	0.005417478431235397
32	0.0062403865473724205
33	0.00809192980868072
34	0.012343621742055336
35	0.015086648795845412
36	0.014400892032397892
37	0.009737746040954766
38	0.005554629783924901
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1458243.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.61183887789033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56134987304776	45.6072615879833
2	12.096473802262652	13.696072517686295
3	3.4041936707973406	5.781529908009393
4	1.3700878682813828	3.1025277459079152
5	0.7114683939216457	2.0138767041701806
6	0.434613696929551	1.4762568350819998
7	0.2528069894736571	1.0018307988701034
8	0.19267541526760187	0.8726167651888069
9	0.14135996409497623	0.720238276001624
>10	0.680642636359756	6.987623039549945
>50	0.06641868358013672	2.665919084490657
>100	0.0742910782331265	9.159708599325457
>500	0.009727091250278763	3.6856244218375522
>1k	0.0038908365001115055	3.22891371589679
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4190	0.2873320838845103	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2619	0.17959969634690515	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1783	0.12227043092269257	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1747	0.1198017065742815	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1741	0.119390252516213	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1715	0.11760728493124945	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1618	0.11095544432580852	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1590	0.10903532538815547	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1576	0.10807526591932895	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1561	0.10704663077415767	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1551	0.10636087401071015	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1468	0.10066909287409574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	6.857567634475186E-5	0.0	0.0	0.0
14	0.0	6.857567634475186E-5	0.0	0.0	0.0
15	0.0	6.857567634475186E-5	0.0	0.0	6.857567634475186E-5
16	0.0	6.857567634475186E-5	0.0	0.0	6.857567634475186E-5
17	0.0	6.857567634475186E-5	0.0	0.0	6.857567634475186E-5
18	0.0	6.857567634475186E-5	0.0	6.857567634475186E-5	6.857567634475186E-5
19	0.0	6.857567634475186E-5	0.0	6.857567634475186E-5	6.857567634475186E-5
20	0.0	6.857567634475186E-5	0.0	1.3715135268950373E-4	2.057270290342556E-4
21	0.0	6.857567634475186E-5	0.0	2.057270290342556E-4	2.7430270537900746E-4
22	0.0	6.857567634475186E-5	0.0	3.4287838172375934E-4	2.7430270537900746E-4
23	0.0	6.857567634475186E-5	0.0	8.229081161370224E-4	2.7430270537900746E-4
24	0.0	6.857567634475186E-5	0.0	0.002125845966687308	2.7430270537900746E-4
25	0.0	6.857567634475186E-5	0.0	0.002537300024755819	2.7430270537900746E-4
26	0.0	6.857567634475186E-5	0.0	0.0027430270537900747	2.7430270537900746E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAAT	135	4.3655746E-10	16.610432	1
TCTAGCG	390	0.0	16.409306	28
GTATTAG	255	0.0	16.331263	1
CATCGTT	315	0.0	16.253027	28
ATCGTTT	325	0.0	15.752934	29
AACGGAC	65	0.0041619944	14.769389	15
TAGCCGA	65	0.004164842	14.76787	19
CTAGCGG	430	0.0	14.510787	29
TACTATA	100	2.3172839E-5	14.406083	2
TAGCGGC	435	0.0	14.343997	30
GCATCGT	360	0.0	14.221399	27
CGTCGTA	360	0.0	13.7779255	10
CGCTTCG	375	0.0	13.652543	32
GTAACAC	130	1.3389254E-6	13.53675	3
GTCTTAC	95	2.4056747E-4	13.48817	1
GTATCAA	3780	0.0	13.390041	1
TACCGTC	335	0.0	13.371443	7
CGGCATC	400	0.0	13.199689	25
TTTCGTT	500	0.0	13.119242	28
ACCGTCG	355	0.0	13.068769	8
>>END_MODULE