FastQCFastQC Report
Thu 2 Feb 2017
SRR4063087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458243
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46850.3212770436751625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT30650.21018444799666447No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19060.13070523911309706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17500.12000743360331577No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17250.11829304169469698No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17160.1176758606075942No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16630.11404134976132237No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16460.11287556326346158No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16350.1121212308236693No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16180.11095544432580852No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16070.11020111188601625No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15860.10876102268277646No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15560.1067037523924339No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15490.10622372265802063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG400.004481435820.0020145
GTATTAT1650.017.45811
TCTAGCG3200.015.99941628
GATTGCG600.002442922415.99941612
TAATACC1652.910383E-1115.5161834
TAGCGGC3300.015.51458630
ACCGTCG3700.015.136148
ATTGTAC1501.9717845E-914.9343253
CTAGTAT756.24263E-414.9343253
CTAGCGG3550.014.87269829
GCGCAAA2800.014.85660322
GTATAAC1308.2933184E-814.7722381
TAACATA650.00416045514.7702114
CTAGGAC1308.3073246E-814.7702113
TCTATAG1103.5413523E-614.5464213
CGAGCCG4950.014.54492615
ATCGTTT3550.014.42200929
GTATTAG2900.014.3477491
GTAATAC1452.0290827E-814.3457813
TACCGTC3850.014.1312927
Produced by FastQC (version 0.11.5)