##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063087_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1458243
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21972195306269	32.0	32.0	32.0	32.0	32.0
2	31.29949329432749	32.0	32.0	32.0	32.0	32.0
3	31.369827936770484	32.0	32.0	32.0	32.0	32.0
4	31.463066855112626	32.0	32.0	32.0	32.0	32.0
5	31.3933706522164	32.0	32.0	32.0	32.0	32.0
6	34.91392243953854	36.0	36.0	36.0	36.0	36.0
7	34.93948059411223	36.0	36.0	36.0	36.0	36.0
8	34.88242425987987	36.0	36.0	36.0	32.0	36.0
9	34.97410171007164	36.0	36.0	36.0	36.0	36.0
10	34.83510841471552	36.0	36.0	36.0	32.0	36.0
11	35.00819067878262	36.0	36.0	36.0	36.0	36.0
12	34.90873948992041	36.0	36.0	36.0	32.0	36.0
13	34.96002723825865	36.0	36.0	36.0	36.0	36.0
14	34.896784692263225	36.0	36.0	36.0	32.0	36.0
15	34.86822155155211	36.0	36.0	36.0	32.0	36.0
16	34.887725159661315	36.0	36.0	36.0	32.0	36.0
17	34.85076149859797	36.0	36.0	36.0	32.0	36.0
18	34.8534606372189	36.0	36.0	36.0	32.0	36.0
19	34.83778012306591	36.0	36.0	36.0	32.0	36.0
20	34.828516234948495	36.0	36.0	36.0	32.0	36.0
21	34.822156526724285	36.0	36.0	36.0	32.0	36.0
22	34.81088748583055	36.0	36.0	36.0	32.0	36.0
23	34.752670165397674	36.0	36.0	36.0	32.0	36.0
24	34.72523166577861	36.0	36.0	36.0	32.0	36.0
25	34.703792852082955	36.0	36.0	36.0	32.0	36.0
26	34.645445923621786	36.0	36.0	36.0	32.0	36.0
27	34.63282662766082	36.0	36.0	36.0	32.0	36.0
28	34.60643184983573	36.0	36.0	36.0	32.0	36.0
29	34.56899021630826	36.0	36.0	36.0	32.0	36.0
30	34.54595221784024	36.0	36.0	36.0	32.0	36.0
31	34.53673770420979	36.0	36.0	36.0	32.0	36.0
32	34.51289119851767	36.0	36.0	36.0	32.0	36.0
33	34.465111781781225	36.0	36.0	36.0	32.0	36.0
34	34.460086556218684	36.0	36.0	36.0	32.0	36.0
35	34.42514450609398	36.0	36.0	36.0	32.0	36.0
36	34.401088844589005	36.0	36.0	36.0	32.0	36.0
37	34.397228034010794	36.0	36.0	36.0	32.0	36.0
38	33.957853389318515	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	1.0
20	12.0
21	24.0
22	113.0
23	337.0
24	946.0
25	2317.0
26	5049.0
27	9788.0
28	17235.0
29	27320.0
30	41305.0
31	59114.0
32	84598.0
33	135130.0
34	326944.0
35	748008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99647147172891	18.392376458646954	11.9952815792993	26.615870490324838
2	15.752450037476606	21.204696336618795	36.516067623845956	26.526786002058643
3	17.830361606398935	25.415517166891938	28.79616085933552	27.957960367373612
4	12.285031661498328	16.39019145022966	36.04836796188277	35.276408926389244
5	14.206215429263455	36.83805568623041	33.15595079554697	15.79977808895917
6	33.564753699834256	36.07112953208695	16.810870082517223	13.553246685561577
7	29.390998320577076	30.816553390767904	21.43002621655298	18.362422072102035
8	27.9603760775197	32.51294395183135	19.783227381515694	19.743452589133252
9	27.774413639914115	14.136488292685936	18.414460108449443	39.67463795895051
10	15.83289330215398	26.558499002215015	31.423575156182494	26.18503253944851
11	37.38374991684788	21.242364578639954	22.34353858360919	19.030346920902964
12	24.97435264428352	23.956687513715163	28.333950515690148	22.73500932631117
13	29.50504785899734	19.48709473461881	25.160775783443352	25.8470816229405
14	23.968021790606915	19.671892818960902	25.074353177076798	31.285732213355388
15	25.22302524339222	26.56313111052136	22.61577802876475	25.59806561732167
16	25.809895881550602	25.68213939652033	23.487717753488273	25.02024696844079
17	24.187532530586466	25.672675953184758	25.047060057891585	25.09273145833719
18	25.23790616515903	24.469241409010706	26.094622089734017	24.198230336096245
19	25.728976583463798	24.932607254072195	25.445621888807285	23.892794273656722
20	25.922908596166756	23.946214725529284	24.978072927488764	25.152803750815195
21	27.24567097711692	24.14617905569663	24.323859120487107	24.284290846699342
22	26.031669618849534	24.05422141577227	24.91045731061284	25.003651654765356
23	24.32435583217554	23.956788946660286	25.54754436518593	26.17131085597824
24	24.918206362039797	24.859437007412343	25.13950006960431	25.082856560943544
25	24.954414319149826	24.154136176206574	25.418465920974764	25.47298358366884
26	24.917126238306125	24.93961907557182	25.643411724528576	24.49984296159348
27	25.60987819580015	24.52459879773042	24.639668861546987	25.22585414492245
28	24.630599975449908	24.398608462375613	25.758807002673766	25.211984559500717
29	24.728388889917525	24.658167397340495	25.61959837969392	24.99384533304806
30	24.685666243554742	24.735315033228346	25.79666077601607	24.782357947200843
31	25.1840742592284	24.659127456809323	24.67894582727296	25.477852456689316
32	24.882066980606112	24.711519273536712	24.693278143629012	25.71313560222816
33	24.433719208664126	24.205705084817826	25.442947437429837	25.917628269088212
34	25.217607764960988	24.374675551331293	25.585241965845203	24.822474717862526
35	25.9667970290274	24.212631228128643	25.349478790571943	24.471092952272013
36	24.900856715924576	24.920812237740897	24.9508483839799	25.227482662354628
37	25.721158956360497	24.90942867546767	24.823503353007695	24.54590901516414
38	24.748893017144606	24.57347643705473	25.42086606964683	25.256764476153837
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	281.0
1	227.5
2	174.0
3	174.0
4	605.0
5	1036.0
6	1036.0
7	1209.5
8	1383.0
9	1367.5
10	1352.0
11	1352.0
12	1761.5
13	2171.0
14	2797.5
15	3424.0
16	3424.0
17	5213.5
18	7003.0
19	7003.0
20	8324.0
21	9645.0
22	10098.0
23	10551.0
24	10551.0
25	12148.0
26	13745.0
27	13745.0
28	17480.0
29	21215.0
30	25943.0
31	30671.0
32	30671.0
33	39669.0
34	48667.0
35	48667.0
36	54646.5
37	60626.0
38	69485.5
39	78345.0
40	78345.0
41	84976.0
42	91607.0
43	103091.0
44	114575.0
45	114575.0
46	119094.0
47	123613.0
48	123613.0
49	130949.5
50	138286.0
51	137980.0
52	137674.0
53	137674.0
54	131594.5
55	125515.0
56	125515.0
57	122171.0
58	118827.0
59	105724.0
60	92621.0
61	92621.0
62	87754.5
63	82888.0
64	67953.0
65	53018.0
66	53018.0
67	44528.0
68	36038.0
69	36038.0
70	28941.0
71	21844.0
72	16993.5
73	12143.0
74	12143.0
75	9187.0
76	6231.0
77	6231.0
78	5963.0
79	5695.0
80	4389.0
81	3083.0
82	3083.0
83	2954.5
84	2826.0
85	2826.0
86	1797.0
87	768.0
88	658.5
89	549.0
90	549.0
91	318.0
92	87.0
93	60.0
94	33.0
95	33.0
96	21.0
97	9.0
98	9.0
99	16.5
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008777686572128239
2	0.0
3	0.0
4	3.4287838172375934E-4
5	6.857567634475186E-5
6	1.3715135268950373E-4
7	2.7430270537900746E-4
8	0.0022629973193768116
9	0.003565935169927097
10	8.914837924817742E-4
11	0.004937448696822134
12	2.057270290342556E-4
13	6.857567634475186E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.7430270537900746E-4
22	0.0
23	3.4287838172375934E-4
24	0.0
25	0.0
26	6.857567634475186E-5
27	6.857567634475186E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1458243.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.65159307816215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93260297917567	43.13792498618039
2	13.112943954514503	14.332865541177462
3	3.743320901803392	6.137353520590141
4	1.4699414961792663	3.213385779915765
5	0.7608985310011552	2.0792158445023246
6	0.45227153827060657	1.483041604223978
7	0.2812850128024323	1.0760871841064914
8	0.20556242322685436	0.8987451125083994
9	0.1481743822291293	0.7288169437974994
>10	0.7256282939352693	7.1167549922743625
>50	0.07125723253164562	2.7358985007237715
>100	0.08095747969004236	9.477699677168745
>500	0.01111423473604907	4.183540742844007
>1k	0.0040415399040178435	3.3986695699867355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4685	0.3212770436751625	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3065	0.21018444799666447	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1906	0.13070523911309706	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1750	0.12000743360331577	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1725	0.11829304169469698	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1716	0.1176758606075942	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1663	0.11404134976132237	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1646	0.11287556326346158	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1635	0.1121212308236693	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1618	0.11095544432580852	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1607	0.11020111188601625	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1586	0.10876102268277646	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1556	0.1067037523924339	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1549	0.10622372265802063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3715135268950373E-4
2	0.0	0.0	0.0	0.0	1.3715135268950373E-4
3	0.0	0.0	0.0	0.0	1.3715135268950373E-4
4	0.0	0.0	0.0	0.0	1.3715135268950373E-4
5	0.0	0.0	0.0	0.0	1.3715135268950373E-4
6	0.0	0.0	0.0	0.0	1.3715135268950373E-4
7	0.0	0.0	0.0	0.0	1.3715135268950373E-4
8	0.0	0.0	0.0	0.0	1.3715135268950373E-4
9	0.0	0.0	0.0	0.0	1.3715135268950373E-4
10	0.0	0.0	0.0	0.0	1.3715135268950373E-4
11	0.0	0.0	0.0	0.0	1.3715135268950373E-4
12	0.0	0.0	0.0	0.0	2.057270290342556E-4
13	0.0	0.0	0.0	0.0	2.057270290342556E-4
14	0.0	0.0	0.0	6.857567634475186E-5	2.057270290342556E-4
15	0.0	0.0	0.0	6.857567634475186E-5	2.057270290342556E-4
16	0.0	0.0	0.0	6.857567634475186E-5	2.057270290342556E-4
17	0.0	0.0	0.0	6.857567634475186E-5	2.057270290342556E-4
18	0.0	0.0	0.0	6.857567634475186E-5	2.057270290342556E-4
19	0.0	0.0	0.0	6.857567634475186E-5	2.057270290342556E-4
20	0.0	0.0	0.0	1.3715135268950373E-4	2.7430270537900746E-4
21	0.0	0.0	0.0	2.057270290342556E-4	3.4287838172375934E-4
22	0.0	0.0	0.0	3.4287838172375934E-4	3.4287838172375934E-4
23	0.0	0.0	0.0	8.229081161370224E-4	3.4287838172375934E-4
24	0.0	0.0	0.0	0.001988694613997804	3.4287838172375934E-4
25	0.0	0.0	0.0	0.0024001486720663153	3.4287838172375934E-4
26	0.0	0.0	0.0	0.002674451377445323	3.4287838172375934E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	40	0.0044814358	20.002014	5
GTATTAT	165	0.0	17.4581	1
TCTAGCG	320	0.0	15.999416	28
GATTGCG	60	0.0024429224	15.999416	12
TAATACC	165	2.910383E-11	15.516183	4
TAGCGGC	330	0.0	15.514586	30
ACCGTCG	370	0.0	15.13614	8
ATTGTAC	150	1.9717845E-9	14.934325	3
CTAGTAT	75	6.24263E-4	14.934325	3
CTAGCGG	355	0.0	14.872698	29
GCGCAAA	280	0.0	14.856603	22
GTATAAC	130	8.2933184E-8	14.772238	1
TAACATA	65	0.004160455	14.770211	4
CTAGGAC	130	8.3073246E-8	14.770211	3
TCTATAG	110	3.5413523E-6	14.546421	3
CGAGCCG	495	0.0	14.544926	15
ATCGTTT	355	0.0	14.422009	29
GTATTAG	290	0.0	14.347749	1
GTAATAC	145	2.0290827E-8	14.345781	3
TACCGTC	385	0.0	14.131292	7
>>END_MODULE