FastQCFastQC Report
Thu 2 Feb 2017
SRR4063086_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063086_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799460
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33520.4192830160358242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21800.27268406174167564No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12130.15172741600580392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11400.14259625247041752No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT9960.12458409426362796No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9910.12395867210366997No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9730.12170715232782128No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9660.1208315613038801No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9600.12008105471193055No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9470.11845495709603983No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9180.11482750856828358No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9100.11382683311235084No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8580.10732244264878793No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8340.10432041628098968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC801.9826984E-1024.0207081
ATAGCGT1400.019.4258776
AATAGCG1450.018.7560165
CGTATAT1301.2732926E-1118.46128710
AGCGTAT1352.3646862E-1117.775318
TAGCGTA1352.3646862E-1117.775317
TCTAGCG1900.017.6839728
CTAGCGG1900.017.6839729
CAACGGA652.0996144E-417.23053414
AACGGAC652.0996144E-417.23053415
GGGTTAG854.9393675E-616.9557951
GCGTATA1456.91216E-1116.5514989
CTATAAG805.576131E-516.0138051
TTCTATA1102.010056E-716.0047872
TCGTTTA1800.015.99978230
CTAAACA908.585559E-615.9977794
TTCGTTC2450.015.67325529
ATCGTTT1850.015.56735429
CATCGTT1850.015.56735428
CGTTATT1357.86531E-915.4120172
Produced by FastQC (version 0.11.5)