##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063086_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	799460
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16938308358142	32.0	32.0	32.0	32.0	32.0
2	30.815869461886773	32.0	32.0	32.0	32.0	32.0
3	30.863405298576538	32.0	32.0	32.0	32.0	32.0
4	30.919672028619317	32.0	32.0	32.0	32.0	32.0
5	30.864981362419634	32.0	32.0	32.0	32.0	32.0
6	34.488267080279186	36.0	36.0	36.0	32.0	36.0
7	34.40150601656118	36.0	36.0	36.0	32.0	36.0
8	34.391397943611935	36.0	36.0	36.0	32.0	36.0
9	34.48299477147074	36.0	36.0	36.0	32.0	36.0
10	34.25419408100468	36.0	36.0	36.0	32.0	36.0
11	34.51210442048383	36.0	36.0	36.0	32.0	36.0
12	34.33447952367848	36.0	36.0	36.0	32.0	36.0
13	34.41678758161759	36.0	36.0	36.0	32.0	36.0
14	34.326431591324145	36.0	36.0	36.0	32.0	36.0
15	34.26956695769645	36.0	36.0	36.0	32.0	36.0
16	34.29180071548295	36.0	36.0	36.0	32.0	36.0
17	34.21402571735922	36.0	36.0	36.0	32.0	36.0
18	34.2405598779176	36.0	36.0	36.0	32.0	36.0
19	34.23406049083131	36.0	36.0	36.0	32.0	36.0
20	34.20267180346734	36.0	36.0	36.0	32.0	36.0
21	34.18140369749581	36.0	36.0	36.0	32.0	36.0
22	34.151743676981965	36.0	36.0	36.0	32.0	36.0
23	34.0922685312586	36.0	36.0	36.0	32.0	36.0
24	34.083282465664325	36.0	36.0	36.0	32.0	36.0
25	34.06259350061291	36.0	36.0	36.0	32.0	36.0
26	34.03242938983814	36.0	36.0	36.0	32.0	36.0
27	34.01960448302604	36.0	36.0	36.0	32.0	36.0
28	34.00822054887049	36.0	36.0	36.0	32.0	36.0
29	33.97264528556776	36.0	36.0	36.0	32.0	36.0
30	33.93730393015285	36.0	36.0	36.0	32.0	36.0
31	33.95758637080029	36.0	36.0	36.0	32.0	36.0
32	33.90423160633428	36.0	36.0	36.0	32.0	36.0
33	33.86502639281515	36.0	36.0	36.0	32.0	36.0
34	33.86914667400495	36.0	36.0	36.0	32.0	36.0
35	33.80366372301303	36.0	36.0	36.0	32.0	36.0
36	33.76832862181973	36.0	36.0	36.0	32.0	36.0
37	33.76335401395942	36.0	36.0	36.0	32.0	36.0
38	33.15998549020589	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	5.0
11	5.0
12	3.0
13	1.0
14	229.0
15	378.0
16	422.0
17	480.0
18	631.0
19	740.0
20	970.0
21	1323.0
22	1873.0
23	2745.0
24	3871.0
25	5582.0
26	8196.0
27	11429.0
28	15828.0
29	21452.0
30	28597.0
31	38179.0
32	51723.0
33	76746.0
34	167727.0
35	360321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.485648878096555	17.513140114889126	11.508198404776902	25.49301260223742
2	16.347869834777974	20.43762186407291	37.70571406402134	25.508794237127773
3	18.934607007794916	24.82364170650027	28.539456845228788	27.702294440476027
4	12.28770891369258	15.646714608979115	36.75342398925238	35.312152488075924
5	14.267040920223451	36.72600686965419	33.74561265257211	15.26133955755024
6	32.90416757353107	36.14211128039246	17.518493779535635	13.43522736654083
7	29.445000375253294	31.075475946263726	21.290746253721263	18.188777424761714
8	27.886983572641	32.57778072421475	20.13861874092355	19.396616962220705
9	27.642283541390437	14.532809646511396	19.091011432717085	38.73389537938108
10	15.706015825721927	26.677574056423225	31.80492283522086	25.811487282633983
11	36.89578697327784	21.70244756075747	22.447835947008503	18.95392951895618
12	24.77728326866355	24.318840797252097	28.90051360567193	22.003362328412425
13	29.34933953164856	19.78540251727326	25.533022166566376	25.332235784511802
14	23.695199113328346	20.05043820694176	25.586631873644283	30.667730806085608
15	24.871727824020958	27.092109493955164	23.38550965435012	24.650653027673762
16	25.259325977632663	26.140633991343293	24.22840201155896	24.371638019465085
17	23.530898363313806	25.916116392134096	25.893471521259904	24.65951372329219
18	24.61913345433667	24.830534870601067	27.099910310760084	23.450421364302183
19	25.060012784380536	25.391255833968174	26.392490489896964	23.156240891754326
20	25.317540335190497	24.076274727116704	26.239201627167493	24.366983310525303
21	26.555760705314878	24.519649344021136	25.061795110430612	23.86279484023337
22	25.234735617517916	24.707721944934388	25.752742647702682	24.30479978984501
23	23.617044432463132	24.465313056281452	26.344363786022644	25.573278725232772
24	23.98158515302963	25.583001294793927	26.15946606951855	24.27594748265789
25	24.40753876659974	24.74506933888795	26.080804668881008	24.766587225631305
26	23.852262946133678	25.688467081690252	26.592619652901494	23.866650319274576
27	24.847699416816987	25.31304493586488	25.56197976997921	24.277275877338926
28	23.817082842353603	25.09363014778177	26.592713235312516	24.496573774552115
29	23.770630231443896	25.56290993065815	26.604351160221825	24.06210867767613
30	23.922310811791224	25.47714530885293	26.7257662219398	23.874777657416047
31	24.231131992864846	25.597369099174156	25.446134832416423	24.725364075544583
32	23.895818947831916	25.5401443053429	25.495611796611527	25.068424950213657
33	23.634225669251936	25.055166374781084	26.250562922191644	25.06004503377533
34	24.29714102714861	25.367223015894858	26.38363827876174	23.951997678194797
35	25.215175367924857	25.080190355139436	26.134924963845535	23.56970931309017
36	24.039272301467957	25.94121164657931	25.696260173993107	24.32325587795962
37	24.887383302080455	25.816713431316014	25.624567640697375	23.671335625906156
38	23.77929944034285	25.610256461575798	26.131010310000523	24.47943378808083
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	762.0
1	550.5
2	339.0
3	339.0
4	668.0
5	997.0
6	997.0
7	1180.5
8	1364.0
9	1245.5
10	1127.0
11	1127.0
12	1467.5
13	1808.0
14	2291.5
15	2775.0
16	2775.0
17	3991.5
18	5208.0
19	5208.0
20	6287.0
21	7366.0
22	7399.5
23	7433.0
24	7433.0
25	8472.5
26	9512.0
27	9512.0
28	12009.5
29	14507.0
30	17479.0
31	20451.0
32	20451.0
33	25324.5
34	30198.0
35	30198.0
36	33049.5
37	35901.0
38	40890.5
39	45880.0
40	45880.0
41	49106.5
42	52333.0
43	58686.5
44	65040.0
45	65040.0
46	67373.0
47	69706.0
48	69706.0
49	71398.5
50	73091.0
51	72823.0
52	72555.0
53	72555.0
54	67681.5
55	62808.0
56	62808.0
57	60330.0
58	57852.0
59	51467.0
60	45082.0
61	45082.0
62	42817.0
63	40552.0
64	33655.0
65	26758.0
66	26758.0
67	22520.0
68	18282.0
69	18282.0
70	14811.5
71	11341.0
72	8897.5
73	6454.0
74	6454.0
75	4928.5
76	3403.0
77	3403.0
78	3261.0
79	3119.0
80	2434.5
81	1750.0
82	1750.0
83	1796.5
84	1843.0
85	1843.0
86	1225.5
87	608.0
88	525.5
89	443.0
90	443.0
91	314.0
92	185.0
93	164.5
94	144.0
95	144.0
96	128.5
97	113.0
98	113.0
99	241.5
100	370.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1189552948240062
2	0.05178495484452005
3	0.011632852175218271
4	0.003377279663773047
5	2.5016886398318864E-4
6	2.5016886398318864E-4
7	0.0
8	1.2508443199159432E-4
9	0.0
10	2.5016886398318864E-4
11	0.0013759287519075378
12	0.0017511820478823207
13	0.012883696495134215
14	0.008255572511445226
15	0.02289045105446176
16	0.010006754559327546
17	0.02026367798263828
18	0.004127786255722613
19	0.005878968303604933
20	0.004252870687714207
21	0.005503715007630151
22	0.0062542215995797165
23	0.008380656943436818
24	0.013133865359117404
25	0.01563555399894929
26	0.01851249593475596
27	0.006754559327546094
28	0.004753208415680585
29	0.010882345583268706
30	0.002251519775848698
31	0.004753208415680585
32	0.005503715007630151
33	0.007505065919495659
34	0.010256923423310735
35	0.014009456383058566
36	0.014759962975008131
37	0.008380656943436818
38	0.005253546143646962
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	799460.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.73751886413011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.62244103352023	49.95397148704524
2	10.70280189286756	12.787176599484468
3	2.5636733211537917	4.594424501516752
4	0.9536566148875791	2.278763200869969
5	0.49804899872218156	1.4876105728213718
6	0.31333127463232197	1.123057975744216
7	0.21579708787625046	0.90238278254823
8	0.16225209159730875	0.7754029906031039
9	0.1106151888777379	0.5947089239018865
>10	0.6812525978843289	7.78443873989389
>50	0.0849052878432444	3.589301334558856
>100	0.08279739442256694	9.887187611550209
>500	0.007584494143437796	3.2492047165113216
>1k	8.427215714930886E-4	0.9923685629504502
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3352	0.4192830160358242	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2180	0.27268406174167564	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1213	0.15172741600580392	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1140	0.14259625247041752	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	996	0.12458409426362796	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	991	0.12395867210366997	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	973	0.12170715232782128	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	966	0.1208315613038801	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	960	0.12008105471193055	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	947	0.11845495709603983	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	918	0.11482750856828358	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	910	0.11382683311235084	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	858	0.10732244264878793	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	834	0.10432041628098968	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.2508443199159432E-4	0.0
5	0.0	0.0	0.0	1.2508443199159432E-4	0.0
6	0.0	0.0	0.0	1.2508443199159432E-4	0.0
7	0.0	0.0	0.0	2.5016886398318864E-4	0.0
8	0.0	0.0	0.0	2.5016886398318864E-4	0.0
9	0.0	0.0	0.0	3.7525329597478297E-4	0.0
10	0.0	0.0	0.0	3.7525329597478297E-4	0.0
11	0.0	0.0	0.0	3.7525329597478297E-4	0.0
12	0.0	0.0	0.0	3.7525329597478297E-4	0.0
13	0.0	0.0	0.0	3.7525329597478297E-4	0.0
14	0.0	0.0	0.0	3.7525329597478297E-4	0.0
15	0.0	0.0	0.0	5.003377279663773E-4	0.0
16	0.0	0.0	0.0	5.003377279663773E-4	0.0
17	0.0	0.0	0.0	6.254221599579716E-4	0.0
18	0.0	0.0	0.0	8.755910239411603E-4	0.0
19	0.0	0.0	0.0	0.0010006754559327546	0.0
20	0.0	0.0	0.0	0.0010006754559327546	0.0
21	0.0	0.0	0.0	0.0012508443199159432	0.0
22	0.0	0.0	0.0	0.002001350911865509	0.0
23	0.0	0.0	0.0	0.003877617391739424	0.0
24	0.0	0.0	0.0	0.006129137167588122	0.0
25	0.0	0.0	0.0	0.007004728191529282	0.0
26	0.0	0.0	0.0	0.009006079103394791	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAC	80	1.9826984E-10	24.020708	1
ATAGCGT	140	0.0	19.425877	6
AATAGCG	145	0.0	18.756016	5
CGTATAT	130	1.2732926E-11	18.461287	10
AGCGTAT	135	2.3646862E-11	17.77531	8
TAGCGTA	135	2.3646862E-11	17.77531	7
TCTAGCG	190	0.0	17.68397	28
CTAGCGG	190	0.0	17.68397	29
CAACGGA	65	2.0996144E-4	17.230534	14
AACGGAC	65	2.0996144E-4	17.230534	15
GGGTTAG	85	4.9393675E-6	16.955795	1
GCGTATA	145	6.91216E-11	16.551498	9
CTATAAG	80	5.576131E-5	16.013805	1
TTCTATA	110	2.010056E-7	16.004787	2
TCGTTTA	180	0.0	15.999782	30
CTAAACA	90	8.585559E-6	15.997779	4
TTCGTTC	245	0.0	15.673255	29
ATCGTTT	185	0.0	15.567354	29
CATCGTT	185	0.0	15.567354	28
CGTTATT	135	7.86531E-9	15.412017	2
>>END_MODULE