FastQCFastQC Report
Thu 2 Feb 2017
SRR4063086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799460
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38680.48382658294348685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26160.32722087409001077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14930.18675105696345035No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12630.1579816376053836No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.15497961123758536No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12260.15335351362169464No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10820.13534135541490505No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10460.13083831586320765No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10290.12871188051935056No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9520.11908037925599779No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9250.11570309959222476No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9190.11495259300027519No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9050.11320141095239288No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA8600.10757261151277112No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8310.10394516298501488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC801.2636883E-720.00093
CTAGCGG2350.019.06349629
TCTAGCG2400.018.66633828
CGCGCCA450.00884888117.77746610
TGTTAGC450.00884888117.7774662
CAAGACG2300.016.6964044
AGCGGCG2850.016.28041531
CTATTAG703.691616E-416.001721
TACTAAC703.691616E-416.001721
CCCGTCC908.5706E-616.000728
TACGACG3400.016.000725
TATACCT600.002440300516.000724
CGCGTAA600.002441319415.99971910
GCGTAAC600.002441319415.99971911
TAGTACG600.002441319415.9997192
TGCGTAG600.002441319415.99971913
TAGCGGC2950.015.72853730
TCCCCGC1850.015.5692411
GACGGTA3300.015.5158498
ACGACGG3500.015.0863916
Produced by FastQC (version 0.11.5)