FastQCFastQC Report
Thu 2 Feb 2017
SRR4063085_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063085_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344303
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30460.22658582179761558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18330.1363531882321173No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16850.12534376550524695No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16250.1208804860213806No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13820.10280420411172184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG3250.020.67694729
TCTAGCG3250.020.18313828
TAGCGGC3750.018.3466930
CGAGCCG3300.016.96908615
AAGACGG4250.016.562265
GCGAAAG4400.016.36243818
CAAGACG4400.016.3612214
GTATTAC1304.7384674E-916.0161061
AACGGAC600.002442782515.99942315
CGGTCCA4750.015.83159610
GTATCAA20450.015.820311
GTGTAGG2300.015.3197541
CGGACCA4700.015.3191669
GAGCGAA4600.015.30379716
CCGATAA1901.8189894E-1215.1579139
CGAAAGC4750.015.15734919
ACGAACG1901.8189894E-1215.15734915
GACGGAC4450.015.100587
AGCGGCG4350.015.08047931
AGGCCCG3850.014.96105710
Produced by FastQC (version 0.11.5)