##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063085_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1344303
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203798548392736	32.0	32.0	32.0	32.0	32.0
2	30.787404327744564	32.0	32.0	32.0	32.0	32.0
3	30.838249263744856	32.0	32.0	32.0	32.0	32.0
4	30.908278118846717	32.0	32.0	32.0	32.0	32.0
5	30.80044082323702	32.0	32.0	32.0	32.0	32.0
6	34.438391493584405	36.0	36.0	36.0	32.0	36.0
7	34.383062449462656	36.0	36.0	36.0	32.0	36.0
8	34.358521851100534	36.0	36.0	36.0	32.0	36.0
9	34.52467412480669	36.0	36.0	36.0	32.0	36.0
10	34.21687670116038	36.0	36.0	36.0	32.0	36.0
11	34.4979859451329	36.0	36.0	36.0	32.0	36.0
12	34.31132787771804	36.0	36.0	36.0	32.0	36.0
13	34.40076827917515	36.0	36.0	36.0	32.0	36.0
14	34.29272492882929	36.0	36.0	36.0	32.0	36.0
15	34.236009292547884	36.0	36.0	36.0	32.0	36.0
16	34.25272873749445	36.0	36.0	36.0	32.0	36.0
17	34.177927148864505	36.0	36.0	36.0	32.0	36.0
18	34.201093801025515	36.0	36.0	36.0	32.0	36.0
19	34.195828618994376	36.0	36.0	36.0	32.0	36.0
20	34.181256011479555	36.0	36.0	36.0	32.0	36.0
21	34.17196569523389	36.0	36.0	36.0	32.0	36.0
22	34.139016278324156	36.0	36.0	36.0	32.0	36.0
23	34.08378021919165	36.0	36.0	36.0	32.0	36.0
24	34.062785696379464	36.0	36.0	36.0	32.0	36.0
25	34.049328164855694	36.0	36.0	36.0	32.0	36.0
26	33.99283346090874	36.0	36.0	36.0	32.0	36.0
27	33.9930551371231	36.0	36.0	36.0	32.0	36.0
28	33.974137527030734	36.0	36.0	36.0	32.0	36.0
29	33.94583066466414	36.0	36.0	36.0	32.0	36.0
30	33.91837033763965	36.0	36.0	36.0	32.0	36.0
31	33.93548627058037	36.0	36.0	36.0	32.0	36.0
32	33.9029392927041	36.0	36.0	36.0	32.0	36.0
33	33.863506962344054	36.0	36.0	36.0	32.0	36.0
34	33.87294977397209	36.0	36.0	36.0	32.0	36.0
35	33.840478671847045	36.0	36.0	36.0	32.0	36.0
36	33.796679766391954	36.0	36.0	36.0	32.0	36.0
37	33.78965084508478	36.0	36.0	36.0	32.0	36.0
38	33.19204896515146	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	3.0
10	13.0
11	8.0
12	4.0
13	7.0
14	104.0
15	283.0
16	394.0
17	478.0
18	589.0
19	732.0
20	1102.0
21	1579.0
22	2464.0
23	3952.0
24	5931.0
25	9270.0
26	13517.0
27	19845.0
28	27683.0
29	37943.0
30	50732.0
31	68703.0
32	93779.0
33	138033.0
34	295853.0
35	571296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.08941688879753	16.868390536219806	11.201800581971689	25.840391993010975
2	17.108771083691707	18.93293558821472	37.353431965786775	26.604861362306803
3	19.7926423444985	22.586004102217682	27.697706582072996	29.92364697121082
4	12.783649640249237	14.640204602667334	35.64188284444127	36.934262912642154
5	14.932901882020383	36.11530164397828	33.498549430930595	15.453247043070743
6	35.26918882569192	34.74527225362234	16.405291088731815	13.580247831953926
7	31.15860040481945	29.801689053732677	20.070326407067455	18.96938413438042
8	28.536455458536725	32.69914855188358	18.98217508320328	19.782220906376413
9	27.267405142151947	14.431143025259969	18.256699950383133	40.044751882204956
10	15.867475714072112	26.34953436628959	31.294126223594937	26.488863696043357
11	38.11907813642575	21.155981988831172	21.585432169113954	19.13950770562912
12	24.97896623215477	23.30928305165566	28.317811863268233	23.39393885292133
13	29.55996226504934	19.066863475662743	25.091212637042155	26.281961622245763
14	23.462850102962708	19.31979145588078	24.965183130780495	32.252175310376025
15	25.32074677095448	26.8529204083713	22.061455103636753	25.76487771703747
16	26.099037832746717	25.746325009132143	23.3163932974892	24.838243860631938
17	24.3503396552109	25.749585196538717	24.91975506134627	24.980320086904115
18	25.19732544973836	24.788168061025452	25.786363304979965	24.22814318425622
19	25.561468205228543	24.883443597264986	25.394379850007326	24.160708347499142
20	25.76378184718114	24.228927744615515	25.067994215284266	24.93929619291908
21	26.841465093009674	24.00910568621835	24.59866911171204	24.550760109059937
22	25.79247290229949	24.088870042627267	25.187433511132934	24.931223543940305
23	24.485783154041872	24.045142763617967	25.540626999360207	25.92844708297996
24	24.592517863147904	24.823600484484878	25.346103140521446	25.237778511845775
25	24.904394718124383	24.37436201144585	25.34983349056744	25.37140977986233
26	24.716207760398028	25.137924851323895	25.607111156083683	24.538756232194395
27	25.413724686447093	24.530219291752438	24.96520211785379	25.090853903946687
28	24.640815339693876	24.571556101099144	25.68781268016887	25.099815879038108
29	24.48776292579788	25.01813409634171	25.54017288210494	24.95393009575547
30	24.610196776238073	25.12006534411293	25.700678733704883	24.56905914594411
31	25.129015051818037	24.868418409743025	24.749836894622394	25.25272964381654
32	24.82249665228389	24.775703020383872	24.909537271239397	25.492263056092845
33	24.700691350346418	24.33771939939636	25.310133084755698	25.651456165501518
34	25.291263436060422	24.64192824578319	25.3379846206954	24.728823697460985
35	25.662248576179515	24.418809682351323	25.45891473444957	24.460027007019594
36	24.651809548751128	25.04106861732679	25.130869017894607	25.17625281602747
37	25.548702917041744	24.83941763318628	24.94580298622942	24.66607646354256
38	24.793355229632162	24.526284857705054	25.408137835093342	25.272222077569435
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	55.5
2	84.0
3	84.0
4	269.0
5	454.0
6	454.0
7	628.0
8	802.0
9	779.0
10	756.0
11	756.0
12	1035.0
13	1314.0
14	1929.5
15	2545.0
16	2545.0
17	3800.5
18	5056.0
19	5056.0
20	6488.0
21	7920.0
22	8674.0
23	9428.0
24	9428.0
25	11208.5
26	12989.0
27	12989.0
28	16238.5
29	19488.0
30	23652.5
31	27817.0
32	27817.0
33	35036.5
34	42256.0
35	42256.0
36	48206.5
37	54157.0
38	62449.0
39	70741.0
40	70741.0
41	77192.5
42	83644.0
43	94554.0
44	105464.0
45	105464.0
46	108855.0
47	112246.0
48	112246.0
49	119014.5
50	125783.0
51	128405.0
52	131027.0
53	131027.0
54	124938.0
55	118849.0
56	118849.0
57	114546.0
58	110243.0
59	98969.0
60	87695.0
61	87695.0
62	81786.5
63	75878.0
64	63674.0
65	51470.0
66	51470.0
67	42887.5
68	34305.0
69	34305.0
70	27394.5
71	20484.0
72	16101.5
73	11719.0
74	11719.0
75	9016.0
76	6313.0
77	6313.0
78	5693.0
79	5073.0
80	3914.5
81	2756.0
82	2756.0
83	2687.0
84	2618.0
85	2618.0
86	1775.5
87	933.0
88	792.5
89	652.0
90	652.0
91	470.0
92	288.0
93	251.5
94	215.0
95	215.0
96	189.0
97	163.0
98	163.0
99	407.0
100	651.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12095487401277837
2	0.05422884572897629
3	0.011306974692461447
4	0.002901131664513134
5	2.2316397419331805E-4
6	3.719399569888634E-4
7	0.0
8	3.719399569888634E-4
9	1.4877598279554533E-4
10	4.463279483866361E-4
11	0.0019340877763420895
12	0.0026779676903198163
13	0.01420810635697458
14	0.00944727490751713
15	0.024548037161264986
16	0.011158198709665902
17	0.021795681479547392
18	0.005727875337628496
19	0.007736351105368358
20	0.00528154738924186
21	0.006546143243003995
22	0.006918083199992858
23	0.009149722941926038
24	0.014505658322565671
25	0.017481177978476578
26	0.020382309642989715
27	0.008331455036550538
28	0.005802263329026268
29	0.011158198709665902
30	0.0026035796989220435
31	0.005951039311821814
32	0.0076619631139705855
33	0.009075334950528266
34	0.011827690632245855
35	0.015472702210736716
36	0.015695866184930032
37	0.009893602855903766
38	0.006546143243003995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1344303.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.84316301383027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.62678710733776	48.24961964145803
2	12.109479575939595	14.49339120551203
3	3.39866451811213	6.101605043601153
4	1.3517073807556386	3.2356178053422893
5	0.6575285045337207	1.9674292741525743
6	0.412237713948168	1.4801765229749368
7	0.26703905293345015	1.1186323103028717
8	0.19477208847740154	0.932462227303785
9	0.1462497869452515	0.7876844856812368
>10	0.7125340961497479	7.692528338244717
>50	0.057878020267103446	2.4224387976690216
>100	0.05713785805326086	7.35030740969735
>500	0.005738713142990772	2.3587916659144903
>1k	0.002245583403778998	1.8093152721455188
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3046	0.22658582179761558	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1833	0.1363531882321173	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1685	0.12534376550524695	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1625	0.1208804860213806	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1382	0.10280420411172184	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4877598279554536E-4
2	0.0	0.0	0.0	0.0	1.4877598279554536E-4
3	0.0	0.0	0.0	0.0	1.4877598279554536E-4
4	0.0	0.0	0.0	0.0	1.4877598279554536E-4
5	0.0	0.0	0.0	0.0	1.4877598279554536E-4
6	0.0	0.0	0.0	0.0	2.2316397419331802E-4
7	0.0	0.0	0.0	0.0	2.2316397419331802E-4
8	0.0	0.0	0.0	0.0	2.2316397419331802E-4
9	0.0	0.0	0.0	0.0	2.2316397419331802E-4
10	0.0	0.0	0.0	7.438799139777268E-5	2.2316397419331802E-4
11	0.0	0.0	0.0	7.438799139777268E-5	2.2316397419331802E-4
12	0.0	0.0	0.0	7.438799139777268E-5	5.207159397844088E-4
13	0.0	0.0	0.0	7.438799139777268E-5	6.694919225799541E-4
14	0.0	0.0	0.0	1.4877598279554536E-4	6.694919225799541E-4
15	0.0	0.0	0.0	2.2316397419331802E-4	6.694919225799541E-4
16	0.0	0.0	0.0	2.2316397419331802E-4	6.694919225799541E-4
17	0.0	0.0	0.0	2.2316397419331802E-4	7.438799139777268E-4
18	0.0	0.0	0.0	2.975519655910907E-4	8.182679053754994E-4
19	0.0	0.0	0.0	3.719399569888634E-4	8.182679053754994E-4
20	0.0	0.0	0.0	8.182679053754994E-4	0.0010414318795688175
21	0.0	0.0	0.0	0.0010414318795688175	0.0011158198709665902
22	0.0	0.0	0.0	0.0012645958537621355	0.0011902078623643628
23	0.0	0.0	0.0	0.002008475767739862	0.0011902078623643628
24	0.0	0.0	0.0	0.0031242956387064523	0.0011902078623643628
25	7.438799139777268E-5	0.0	0.0	0.0037193995698886336	0.0011902078623643628
26	7.438799139777268E-5	0.0	0.0	0.004388891492468587	0.0013389838451599082
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	325	0.0	20.676947	29
TCTAGCG	325	0.0	20.183138	28
TAGCGGC	375	0.0	18.34669	30
CGAGCCG	330	0.0	16.969086	15
AAGACGG	425	0.0	16.56226	5
GCGAAAG	440	0.0	16.362438	18
CAAGACG	440	0.0	16.361221	4
GTATTAC	130	4.7384674E-9	16.016106	1
AACGGAC	60	0.0024427825	15.999423	15
CGGTCCA	475	0.0	15.831596	10
GTATCAA	2045	0.0	15.82031	1
GTGTAGG	230	0.0	15.319754	1
CGGACCA	470	0.0	15.319166	9
GAGCGAA	460	0.0	15.303797	16
CCGATAA	190	1.8189894E-12	15.157913	9
CGAAAGC	475	0.0	15.157349	19
ACGAACG	190	1.8189894E-12	15.157349	15
GACGGAC	445	0.0	15.10058	7
AGCGGCG	435	0.0	15.080479	31
AGGCCCG	385	0.0	14.961057	10
>>END_MODULE