FastQCFastQC Report
Thu 2 Feb 2017
SRR4063085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344303
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23620.17570443568153904No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18630.1385848279740505No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16790.12489743755686032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15160.11277219495902338No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13950.10377124799989287No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGT507.192587E-419.2016746
TCTAGCG3150.018.79319228
CTAGCGG3150.018.79319229
TGCGATT601.13857765E-418.66621212
CATCGTT2850.017.96447428
CGTTTAT2950.017.89786731
TATACCG450.00884658817.7793275
GTCCTAG1800.017.7773441
TCGTTTA2800.017.71385430
TAGCGGC3350.017.6712130
ATCGTTT3000.017.59957129
TAATACC1505.456968E-1217.0681554
CAAGACG3900.016.821984
TGTGCGA703.6976673E-415.9996110
TACCGTC2950.015.7301857
GCATCGT3300.015.51477127
CGCCCCT2400.015.334675
GTATCAA21300.015.1733381
AAGACGG4450.014.7428585
ATACCGT3200.014.5012656
Produced by FastQC (version 0.11.5)