##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063085_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1344303
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.125009763423872	32.0	32.0	32.0	32.0	32.0
2	31.128982082164512	32.0	32.0	32.0	32.0	32.0
3	31.28120669224126	32.0	32.0	32.0	32.0	32.0
4	31.399122072925522	32.0	32.0	32.0	32.0	32.0
5	31.30158900188425	32.0	32.0	32.0	32.0	32.0
6	34.820024949732314	36.0	36.0	36.0	32.0	36.0
7	34.84857654859061	36.0	36.0	36.0	32.0	36.0
8	34.76718567168265	36.0	36.0	36.0	32.0	36.0
9	34.91715855725978	36.0	36.0	36.0	32.0	36.0
10	34.72279166229637	36.0	36.0	36.0	32.0	36.0
11	34.934099678420715	36.0	36.0	36.0	36.0	36.0
12	34.82139963981334	36.0	36.0	36.0	32.0	36.0
13	34.885979574545324	36.0	36.0	36.0	32.0	36.0
14	34.82073461117025	36.0	36.0	36.0	32.0	36.0
15	34.784581303471015	36.0	36.0	36.0	32.0	36.0
16	34.802890419793755	36.0	36.0	36.0	32.0	36.0
17	34.76647154696523	36.0	36.0	36.0	32.0	36.0
18	34.766159861281274	36.0	36.0	36.0	32.0	36.0
19	34.767442310252974	36.0	36.0	36.0	32.0	36.0
20	34.760341232594136	36.0	36.0	36.0	32.0	36.0
21	34.74691345626693	36.0	36.0	36.0	32.0	36.0
22	34.730562231877784	36.0	36.0	36.0	32.0	36.0
23	34.666471770129206	36.0	36.0	36.0	32.0	36.0
24	34.65042999978427	36.0	36.0	36.0	32.0	36.0
25	34.62557474021854	36.0	36.0	36.0	32.0	36.0
26	34.561735709880885	36.0	36.0	36.0	32.0	36.0
27	34.549425241184466	36.0	36.0	36.0	32.0	36.0
28	34.50992521775225	36.0	36.0	36.0	32.0	36.0
29	34.48237413737826	36.0	36.0	36.0	32.0	36.0
30	34.45940386951454	36.0	36.0	36.0	32.0	36.0
31	34.46358075523152	36.0	36.0	36.0	32.0	36.0
32	34.4307540785076	36.0	36.0	36.0	32.0	36.0
33	34.405022528403194	36.0	36.0	36.0	32.0	36.0
34	34.39632731608871	36.0	36.0	36.0	32.0	36.0
35	34.36844818467265	36.0	36.0	36.0	32.0	36.0
36	34.33599865506512	36.0	36.0	36.0	32.0	36.0
37	34.33839171674838	36.0	36.0	36.0	32.0	36.0
38	33.890655603684586	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	1.0
21	32.0
22	98.0
23	295.0
24	825.0
25	2171.0
26	4638.0
27	9259.0
28	16354.0
29	26092.0
30	40070.0
31	59172.0
32	86151.0
33	138594.0
34	324991.0
35	635558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.237607351374514	18.103622718318327	11.955357302274093	26.703412628033067
2	16.11578639637046	20.016469501295468	36.528743891816056	27.339000210518016
3	18.697049697873172	23.416521424113462	28.066812318353822	29.81961655965954
4	12.305503008626788	15.629089020985628	35.59344224247397	36.47196572791361
5	14.439750562187244	36.70794456309329	33.03012788039601	15.822176994323453
6	35.24257552054857	35.28728270337863	16.03068653421141	13.439455241861396
7	31.044358435139802	29.985888556042962	20.02017408329546	18.949578925521777
8	28.381276580550086	33.08831679515095	18.66070850718944	19.869698117109525
9	27.107938094940533	14.270612950547962	18.0565846284774	40.564864326034105
10	15.557866563117658	26.735208373782164	31.3029693719589	26.403955691141277
11	38.2719934297007	21.101135147158487	21.33018804059719	19.296683382543627
12	24.787547422450345	23.403406977609166	28.243249274715467	23.565796325225026
13	29.521789002768724	19.027123369599984	25.1041060714036	26.346981556227693
14	23.42024082368335	19.23480048768767	24.841944115277585	32.50301457335139
15	25.37114028608134	27.006039561021584	21.796053419504382	25.8267667333927
16	26.16478576630417	25.927785625710868	23.036398788070844	24.87102981991411
17	24.3538101157254	25.999346873435524	24.600629471183208	25.046213539655866
18	25.233596889986853	25.0084244400258	25.467919062889838	24.290059607097508
19	25.548927585522012	24.984843446752702	25.189856751044964	24.27637221668032
20	25.80973188336261	24.318401431820057	24.71183951832288	25.16002716649446
21	26.84603648444282	24.177880069835652	24.294818340265074	24.681265105456447
22	25.763239388738995	24.290431547054496	24.97695831966454	24.969370744541965
23	24.4847694080418	24.050640444364767	25.395205389269222	26.069384758324215
24	24.635889379105752	25.00299411665376	25.093301138210656	25.267815366029833
25	24.962024902142524	24.58792741511952	25.084690791206143	25.365356891531814
26	24.89475958917	25.19015430301056	25.297644950580338	24.617441157239103
27	25.519023612980106	24.607993882331588	24.67576134249496	25.197221162193344
28	24.634252843295002	24.73155233604329	25.52966109574999	25.10453372491172
29	24.624954344370277	24.967734208731216	25.339822941702877	25.06748850519563
30	24.65894965643906	25.118146727337514	25.53174395950913	24.691159656714298
31	25.171334141186918	24.933962060636627	24.608217046305782	25.286486751870672
32	25.0350553409462	24.859276517273262	24.64370011820252	25.46196802357802
33	24.684167185522906	24.46092882333819	25.119039383234288	25.735864607904617
34	25.20629649714387	24.713847994090617	25.213288968335263	24.866566540430245
35	25.667576431801457	24.42961147895973	25.338037629909326	24.56477445932948
36	24.731998663991675	25.150505503595543	24.88769272998721	25.229803102425567
37	25.745609434777727	24.922134370004382	24.68669637723043	24.64555981798746
38	24.82641549294727	24.517705099003052	25.326749495277102	25.329129912772576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	28.0
2	35.0
3	35.0
4	158.0
5	281.0
6	281.0
7	395.0
8	509.0
9	564.0
10	619.0
11	619.0
12	898.5
13	1178.0
14	1709.5
15	2241.0
16	2241.0
17	3373.5
18	4506.0
19	4506.0
20	5856.0
21	7206.0
22	8238.0
23	9270.0
24	9270.0
25	11210.5
26	13151.0
27	13151.0
28	16691.5
29	20232.0
30	24301.5
31	28371.0
32	28371.0
33	35723.0
34	43075.0
35	43075.0
36	48872.0
37	54669.0
38	62662.0
39	70655.0
40	70655.0
41	77058.0
42	83461.0
43	94178.5
44	104896.0
45	104896.0
46	109061.0
47	113226.0
48	113226.0
49	119763.0
50	126300.0
51	128610.0
52	130920.0
53	130920.0
54	125198.0
55	119476.0
56	119476.0
57	114766.0
58	110056.0
59	99096.0
60	88136.0
61	88136.0
62	82243.5
63	76351.0
64	64033.5
65	51716.0
66	51716.0
67	43011.0
68	34306.0
69	34306.0
70	27431.5
71	20557.0
72	16057.0
73	11557.0
74	11557.0
75	8852.5
76	6148.0
77	6148.0
78	5589.0
79	5030.0
80	3857.0
81	2684.0
82	2684.0
83	2466.0
84	2248.0
85	2248.0
86	1466.5
87	685.0
88	534.5
89	384.0
90	384.0
91	228.0
92	72.0
93	51.0
94	30.0
95	30.0
96	27.5
97	25.0
98	25.0
99	22.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008852170976334948
2	0.0
3	0.0
4	1.4877598279554533E-4
5	0.0
6	0.0
7	2.9755196559109066E-4
8	0.002975519655910907
9	0.003793787561286406
10	8.926558967732722E-4
11	0.005355935380639633
12	2.2316397419331805E-4
13	7.438799139777266E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.9755196559109066E-4
22	0.0
23	1.4877598279554533E-4
24	0.0
25	7.438799139777266E-5
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	7.438799139777266E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1344303.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.891505051169524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.06956538460283	45.77456143856513
2	12.968919144205977	15.015804962900187
3	3.712605800440844	6.447850123476671
4	1.4866812080388117	3.4426485065863064
5	0.739143469463378	2.1395063947989055
6	0.45564868726599933	1.5826912968251012
7	0.29780090773756424	1.2068099928172449
8	0.21173417786277296	0.9806088181798357
9	0.15817465275193648	0.8241271837880143
>10	0.7686787003249725	8.01030150433643
>50	0.06087356907174814	2.463350248427694
>100	0.06075755606275424	7.542043546714877
>500	0.007481795149123244	3.0499987469060703
>1k	0.0019349470213249767	1.519697235677453
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2362	0.17570443568153904	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1863	0.1385848279740505	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1679	0.12489743755686032	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1516	0.11277219495902338	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1395	0.10377124799989287	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.438799139777268E-5
2	0.0	0.0	0.0	0.0	7.438799139777268E-5
3	0.0	0.0	0.0	0.0	7.438799139777268E-5
4	0.0	0.0	0.0	0.0	7.438799139777268E-5
5	0.0	0.0	0.0	0.0	7.438799139777268E-5
6	0.0	0.0	0.0	0.0	7.438799139777268E-5
7	0.0	0.0	0.0	0.0	7.438799139777268E-5
8	0.0	0.0	0.0	0.0	7.438799139777268E-5
9	0.0	0.0	0.0	0.0	7.438799139777268E-5
10	0.0	0.0	0.0	0.0	7.438799139777268E-5
11	0.0	0.0	0.0	0.0	7.438799139777268E-5
12	0.0	0.0	0.0	0.0	5.207159397844088E-4
13	0.0	0.0	0.0	0.0	6.694919225799541E-4
14	0.0	0.0	0.0	1.4877598279554536E-4	6.694919225799541E-4
15	0.0	0.0	0.0	2.2316397419331802E-4	8.182679053754994E-4
16	0.0	0.0	0.0	2.2316397419331802E-4	8.926558967732721E-4
17	0.0	0.0	0.0	2.2316397419331802E-4	8.926558967732721E-4
18	0.0	0.0	0.0	4.4632794838663604E-4	0.0011902078623643628
19	0.0	0.0	0.0	4.4632794838663604E-4	0.0011902078623643628
20	0.0	0.0	0.0	6.694919225799541E-4	0.0012645958537621355
21	0.0	0.0	0.0	8.926558967732721E-4	0.0012645958537621355
22	0.0	0.0	0.0	0.0010414318795688175	0.0013389838451599082
23	0.0	0.0	0.0	0.0016365358107509988	0.0013389838451599082
24	0.0	0.0	0.0	0.002752355681717589	0.0013389838451599082
25	0.0	0.0	0.0	0.0033474596128997705	0.0013389838451599082
26	0.0	0.0	0.0	0.004016951535479724	0.0013389838451599082
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCCGT	50	7.192587E-4	19.201674	6
TCTAGCG	315	0.0	18.793192	28
CTAGCGG	315	0.0	18.793192	29
TGCGATT	60	1.13857765E-4	18.666212	12
CATCGTT	285	0.0	17.964474	28
CGTTTAT	295	0.0	17.897867	31
TATACCG	45	0.008846588	17.779327	5
GTCCTAG	180	0.0	17.777344	1
TCGTTTA	280	0.0	17.713854	30
TAGCGGC	335	0.0	17.67121	30
ATCGTTT	300	0.0	17.599571	29
TAATACC	150	5.456968E-12	17.068155	4
CAAGACG	390	0.0	16.82198	4
TGTGCGA	70	3.6976673E-4	15.99961	10
TACCGTC	295	0.0	15.730185	7
GCATCGT	330	0.0	15.514771	27
CGCCCCT	240	0.0	15.33467	5
GTATCAA	2130	0.0	15.173338	1
AAGACGG	445	0.0	14.742858	5
ATACCGT	320	0.0	14.501265	6
>>END_MODULE