FastQCFastQC Report
Thu 2 Feb 2017
SRR4063084_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063084_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425156
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38430.26965469043388934No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT32860.23057124974388768No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28810.202153308129075No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT26010.18250633614846373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25000.17541939268402895No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23980.1682622814625206No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23170.16257869313955806No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22910.16075433145564416No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC22730.15949131182831916No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22680.1591404730429511No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC21810.15303587817754688No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG21800.15296571042047327No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21040.1476329608828788No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20530.1440544052721246No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC20440.1434228954584621No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20420.14328255994431488No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA19080.13388008049645092No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18610.13058219591399117No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17740.12447760104858697No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC17640.12377592347785084No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG16500.11577679917145911No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT16150.11332092767388272No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16090.11289992113144105No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC16050.1126192501031466No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT15760.11058438514801186No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC15720.11030371411971743No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG15260.10707599729433129No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT15150.10630415196652156No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC15110.10602348093822712No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14900.10454995803968127No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC14630.10265542859869375No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG14520.10188358327088404No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT14500.1017432477567368No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG14360.10076089915770625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5700.021.8947428
CTAGCGG6050.020.62809829
TAGCGGC6200.020.38638130
AGCGGCG6700.018.62621331
GTCCTAT4850.017.8213141
ACGCCTA450.00885307317.77715532
CTAGACA1151.8506398E-816.6892094
ACCTCTA7600.016.63274625
CGGTCCA7700.016.41731310
GTATTAC908.552281E-616.006181
CCTATTC5200.015.9943863
GCGGCGC7850.015.897531532
AAGACGG6650.015.8741285
CTATTCC5350.015.844354
CCGGTCC8250.015.7101949
GTATCAA26750.015.677081
CGCCGGT7900.015.5932947
TCCCGAC3900.015.5886492
GCCGGTC8250.015.3201378
CGACCCG3550.015.3185675
Produced by FastQC (version 0.11.5)