##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063084_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1425156
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.228318864741826	32.0	32.0	32.0	32.0	32.0
2	30.848988461614027	32.0	32.0	32.0	32.0	32.0
3	30.885973184689956	32.0	32.0	32.0	32.0	32.0
4	30.96072570301076	32.0	32.0	32.0	32.0	32.0
5	30.841711363527924	32.0	32.0	32.0	32.0	32.0
6	34.49533314247704	36.0	36.0	36.0	32.0	36.0
7	34.427166569835165	36.0	36.0	36.0	32.0	36.0
8	34.40873350005192	36.0	36.0	36.0	32.0	36.0
9	34.56106349059331	36.0	36.0	36.0	32.0	36.0
10	34.260912489580086	36.0	36.0	36.0	32.0	36.0
11	34.53721978506213	36.0	36.0	36.0	32.0	36.0
12	34.33405746458633	36.0	36.0	36.0	32.0	36.0
13	34.452383458372275	36.0	36.0	36.0	32.0	36.0
14	34.32877523583383	36.0	36.0	36.0	32.0	36.0
15	34.267209344099875	36.0	36.0	36.0	32.0	36.0
16	34.292670416431605	36.0	36.0	36.0	32.0	36.0
17	34.19943571089762	36.0	36.0	36.0	32.0	36.0
18	34.22318328660161	36.0	36.0	36.0	32.0	36.0
19	34.220808809702234	36.0	36.0	36.0	32.0	36.0
20	34.2030205816065	36.0	36.0	36.0	32.0	36.0
21	34.18552495305777	36.0	36.0	36.0	32.0	36.0
22	34.16064627310975	36.0	36.0	36.0	32.0	36.0
23	34.10875791843139	36.0	36.0	36.0	32.0	36.0
24	34.078265116239905	36.0	36.0	36.0	32.0	36.0
25	34.0709424091117	36.0	36.0	36.0	32.0	36.0
26	34.014349306321556	36.0	36.0	36.0	32.0	36.0
27	34.02545054716817	36.0	36.0	36.0	32.0	36.0
28	34.00798017901198	36.0	36.0	36.0	32.0	36.0
29	33.97397267386869	36.0	36.0	36.0	32.0	36.0
30	33.94504461265995	36.0	36.0	36.0	32.0	36.0
31	33.97653379700187	36.0	36.0	36.0	32.0	36.0
32	33.91867697290682	36.0	36.0	36.0	32.0	36.0
33	33.874917552885435	36.0	36.0	36.0	32.0	36.0
34	33.896477999601444	36.0	36.0	36.0	32.0	36.0
35	33.852094788219674	36.0	36.0	36.0	32.0	36.0
36	33.8031176937823	36.0	36.0	36.0	32.0	36.0
37	33.807358633019824	36.0	36.0	36.0	32.0	36.0
38	33.20085590630078	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	3.0
7	1.0
8	0.0
9	5.0
10	10.0
11	21.0
12	6.0
13	9.0
14	105.0
15	277.0
16	402.0
17	462.0
18	629.0
19	812.0
20	1144.0
21	1739.0
22	2711.0
23	4020.0
24	6168.0
25	9307.0
26	14037.0
27	20304.0
28	28528.0
29	38496.0
30	52770.0
31	71349.0
32	97760.0
33	145820.0
34	318944.0
35	609316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.02012680236744	16.8424454240503	11.19640316830292	25.941024605279335
2	17.730140944742583	18.883829406032625	36.05899516143074	27.327034487794055
3	19.351069585702646	22.64119562860397	27.884316462349584	30.1234183233438
4	12.7883624911937	14.434895124326722	34.912596588628574	37.86414579585101
5	15.486558626728938	35.596694247350456	32.677005680797556	16.239741445123045
6	35.7979359394647	34.54915686186456	15.970226333752468	13.682680864918268
7	31.492526421706007	29.614483768069977	20.036711822020635	18.85627798820338
8	29.363204320103627	31.450983088809537	18.779203045852686	20.406609545234154
9	27.47741995239806	13.718396353511766	18.386319494341656	40.417864199748514
10	16.825691086118773	25.02708490626938	29.871915057243175	28.275308950368665
11	39.1505488614388	20.54069377433108	21.127867453681485	19.180889910548636
12	25.58838946080994	23.25139163817197	27.13008527708914	24.030133623928947
13	29.59299897890079	18.10559779919786	25.259187261173317	27.042215960728033
14	24.333528654285423	19.243230725506226	23.650864382931992	32.77237623727636
15	25.87505053227328	26.43553766338768	21.39690854781476	26.29250325652428
16	27.031855930407346	24.96389417941891	22.616068458600765	25.388181431572974
17	24.96631266896302	25.295044874332216	23.991766254839046	25.746876201865714
18	26.381902847872098	23.313900287987067	25.39303497948162	24.911161884659215
19	26.185189811416613	23.91207020685648	24.717307371130477	25.185432610596433
20	26.573299968703118	23.000051927565256	23.93727430806519	26.48937379566643
21	28.32337938743761	23.012386119069824	23.607728219734845	25.056506273757723
22	27.054187952618875	22.95458373098299	24.440015718857016	25.551212597541124
23	24.983649214600902	22.785696641703066	25.29354221169285	26.937111932003187
24	25.51579328547638	24.107945521954942	24.565648682998102	25.810612509570575
25	25.603095800541514	23.35219784152971	24.557126194296014	26.487580163632767
26	25.33377968295141	24.927292735168702	25.068636780892867	24.670290800987022
27	26.345351427139867	23.43745833385964	24.275833708767188	25.9413565302333
28	25.2701270814767	23.363133039078782	25.42920698632348	25.93753289312104
29	24.954578724246364	23.806994139620336	25.385036979129023	25.85339015700428
30	24.721127377267628	24.345718550037788	25.532501633203825	25.400652439490763
31	25.95100324614722	24.051172079484996	23.77918620366381	26.218638470703976
32	25.676540513836766	23.768178386070886	23.540887158571948	27.014393941520403
33	24.89872079078133	23.495653748260555	24.62244416047202	26.9831813004861
34	26.367919695376894	23.635448787135765	24.666625496324844	25.330006021162493
35	26.901046089410958	23.188221704144638	25.013790189806347	24.896942016638057
36	24.83900940959824	24.528397224280805	24.249365581980282	26.383227784140672
37	26.606872682462644	23.998108075486105	24.25544068648228	25.139578555568963
38	25.139589536240653	23.484233417142022	24.886689856154533	26.48948719046279
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	58.5
2	98.0
3	98.0
4	291.5
5	485.0
6	485.0
7	674.0
8	863.0
9	842.5
10	822.0
11	822.0
12	1123.0
13	1424.0
14	1988.5
15	2553.0
16	2553.0
17	3792.0
18	5031.0
19	5031.0
20	5990.0
21	6949.0
22	6984.0
23	7019.0
24	7019.0
25	7743.5
26	8468.0
27	8468.0
28	11317.5
29	14167.0
30	18102.5
31	22038.0
32	22038.0
33	30489.0
34	38940.0
35	38940.0
36	44340.0
37	49740.0
38	59385.5
39	69031.0
40	69031.0
41	74002.5
42	78974.0
43	93304.5
44	107635.0
45	107635.0
46	109025.5
47	110416.0
48	110416.0
49	119843.0
50	129270.0
51	135896.0
52	142522.0
53	142522.0
54	137083.0
55	131644.0
56	131644.0
57	128142.5
58	124641.0
59	112339.5
60	100038.0
61	100038.0
62	97301.5
63	94565.0
64	79340.0
65	64115.0
66	64115.0
67	54562.5
68	45010.0
69	45010.0
70	35978.5
71	26947.0
72	20845.5
73	14744.0
74	14744.0
75	11067.5
76	7391.0
77	7391.0
78	7293.0
79	7195.0
80	5510.5
81	3826.0
82	3826.0
83	4029.5
84	4233.0
85	4233.0
86	2832.0
87	1431.0
88	1274.5
89	1118.0
90	1118.0
91	782.5
92	447.0
93	408.0
94	369.0
95	369.0
96	327.0
97	285.0
98	285.0
99	489.0
100	693.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11795199964074107
2	0.053888837432533705
3	0.011577679917145912
4	0.0028067102829444635
5	2.806710282944464E-4
6	2.806710282944464E-4
7	1.403355141472232E-4
8	3.5083878536805793E-4
9	2.806710282944464E-4
10	5.613420565888928E-4
11	0.0016840261697666783
12	0.002596207011723629
13	0.01550707431326816
14	0.01059533131811535
15	0.022734353291850157
16	0.012489860759102862
17	0.020208314037200137
18	0.006455433650772267
19	0.007718453278097275
20	0.00659576916491949
21	0.0065256014078458775
22	0.008139459820538944
23	0.010384828046894517
24	0.015086067770826491
25	0.01810328132499179
26	0.01852428786743346
27	0.009683150476158399
28	0.006034427108330596
29	0.011437344402998689
30	0.003017213554165298
31	0.005753756080036151
32	0.0078587887922445
33	0.009472647204937564
34	0.012770531787397309
35	0.016138584126930665
36	0.015998248612783445
37	0.010665499075188961
38	0.005964259351256985
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1425156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.064128400923835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.64656202629699	34.08946384241497
2	13.648885232713907	12.301502333129854
3	4.608896663857232	6.230877331399555
4	2.0294515271601186	3.658218568135782
5	1.0778207306440069	2.4285525899459532
6	0.6436142869382727	1.7402350120353218
7	0.44604136501799574	1.407032574170601
8	0.29893532686336355	1.0777007962674203
9	0.22838383733894463	0.9262726713484114
>10	1.1472228538016762	9.27476110455915
>50	0.08958780362262146	2.864547142376294
>100	0.10329642162541362	10.17475859230818
>500	0.021598327642449552	6.688048989483509
>1k	0.009703596477042553	7.138028452425157
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3843	0.26965469043388934	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3286	0.23057124974388768	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2881	0.202153308129075	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2601	0.18250633614846373	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2500	0.17541939268402895	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2398	0.1682622814625206	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2317	0.16257869313955806	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2291	0.16075433145564416	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2273	0.15949131182831916	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2268	0.1591404730429511	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2181	0.15303587817754688	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2180	0.15296571042047327	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2104	0.1476329608828788	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2053	0.1440544052721246	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2044	0.1434228954584621	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2042	0.14328255994431488	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1908	0.13388008049645092	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1861	0.13058219591399117	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1774	0.12447760104858697	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1764	0.12377592347785084	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1650	0.11577679917145911	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1615	0.11332092767388272	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1609	0.11289992113144105	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1605	0.1126192501031466	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1576	0.11058438514801186	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1572	0.11030371411971743	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1526	0.10707599729433129	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1515	0.10630415196652156	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1511	0.10602348093822712	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1490	0.10454995803968127	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	1463	0.10265542859869375	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	1452	0.10188358327088404	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1450	0.1017432477567368	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1436	0.10076089915770625	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.016775707361159E-5
2	0.0	0.0	0.0	0.0	1.4033551414722317E-4
3	0.0	0.0	0.0	0.0	1.4033551414722317E-4
4	0.0	0.0	0.0	0.0	1.4033551414722317E-4
5	0.0	7.016775707361159E-5	0.0	0.0	1.4033551414722317E-4
6	0.0	7.016775707361159E-5	0.0	0.0	1.4033551414722317E-4
7	0.0	7.016775707361159E-5	0.0	0.0	1.4033551414722317E-4
8	0.0	7.016775707361159E-5	0.0	0.0	1.4033551414722317E-4
9	0.0	7.016775707361159E-5	0.0	0.0	1.4033551414722317E-4
10	0.0	7.016775707361159E-5	0.0	0.0	1.4033551414722317E-4
11	0.0	7.016775707361159E-5	0.0	0.0	1.4033551414722317E-4
12	0.0	7.016775707361159E-5	0.0	0.0	2.8067102829444635E-4
13	0.0	7.016775707361159E-5	0.0	7.016775707361159E-5	2.8067102829444635E-4
14	0.0	7.016775707361159E-5	0.0	7.016775707361159E-5	4.210065424416695E-4
15	0.0	7.016775707361159E-5	0.0	7.016775707361159E-5	4.911742995152811E-4
16	0.0	7.016775707361159E-5	0.0	2.1050327122083476E-4	4.911742995152811E-4
17	0.0	7.016775707361159E-5	0.0	2.1050327122083476E-4	4.911742995152811E-4
18	0.0	7.016775707361159E-5	0.0	3.5083878536805793E-4	4.911742995152811E-4
19	0.0	7.016775707361159E-5	0.0	3.5083878536805793E-4	5.613420565888927E-4
20	0.0	7.016775707361159E-5	0.0	4.210065424416695E-4	7.016775707361159E-4
21	0.0	7.016775707361159E-5	0.0	4.911742995152811E-4	7.718453278097274E-4
22	0.0	7.016775707361159E-5	0.0	6.315098136625043E-4	7.718453278097274E-4
23	0.0	7.016775707361159E-5	0.0	7.016775707361159E-4	9.121808419569507E-4
24	0.0	7.016775707361159E-5	0.0	0.0012630196273250086	9.121808419569507E-4
25	0.0	7.016775707361159E-5	0.0	0.001543690655619455	9.121808419569507E-4
26	0.0	7.016775707361159E-5	0.0	0.002175200469281959	9.823485990305622E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	570	0.0	21.89474	28
CTAGCGG	605	0.0	20.628098	29
TAGCGGC	620	0.0	20.386381	30
AGCGGCG	670	0.0	18.626213	31
GTCCTAT	485	0.0	17.821314	1
ACGCCTA	45	0.008853073	17.777155	32
CTAGACA	115	1.8506398E-8	16.689209	4
ACCTCTA	760	0.0	16.632746	25
CGGTCCA	770	0.0	16.417313	10
GTATTAC	90	8.552281E-6	16.00618	1
CCTATTC	520	0.0	15.994386	3
GCGGCGC	785	0.0	15.8975315	32
AAGACGG	665	0.0	15.874128	5
CTATTCC	535	0.0	15.84435	4
CCGGTCC	825	0.0	15.710194	9
GTATCAA	2675	0.0	15.67708	1
CGCCGGT	790	0.0	15.593294	7
TCCCGAC	390	0.0	15.588649	2
GCCGGTC	825	0.0	15.320137	8
CGACCCG	355	0.0	15.318567	5
>>END_MODULE