FastQCFastQC Report
Thu 2 Feb 2017
SRR4063084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425156
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33100.23225527591365439No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT32030.22474732590677793No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC31410.220396924968214No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT26240.18412019456115683No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25700.18033113567918177No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24560.17233201137279008No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24540.17219167585864284No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24370.17099882398839145No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC24290.17043748193180255No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG22960.1611051702410122No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22790.1599123183707608No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21830.1531762136916941No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21700.15226403284973714No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20730.14545776041359684No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG20230.14194937255991624No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19670.138019978163794No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG19340.13570444218036481No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC19010.13338890619693564No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17780.12475827207688141No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC17440.1223725683363786No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA17390.12202172955101055No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC16740.11746082534122579No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16360.11479445057242857No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT16030.11247891458899939No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC15930.11177723701826325No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC15750.11051421739093825No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG15560.10918103000653963No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15410.10812851365043546No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15310.10742683607969934No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15280.10721633280847852No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15100.1059533131811535No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC15060.10567264215285904No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14980.10511130009627018No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT14760.1035676094406507No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG14590.10237475757039931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14360.10076089915770625No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG14340.10062056364355902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG5150.020.81528329
TCTAGCG5350.020.03714228
TATGCGA400.004484311719.9997612
TAGCGGC5800.018.48253430
GTATTAC904.3626824E-717.7800581
TACTATA904.3691034E-717.7775632
GTATATG1006.807568E-817.6022591
AAGACGG7100.017.577875
TAAGACA1302.582965E-1017.230564
CAAGACG7550.017.1653564
AGCGGCG6300.017.01566931
TATAGGG854.986192E-616.9409732
CATCGTT5200.016.30749528
GTATCAA28300.016.2847751
ATCGTTT5150.016.15514629
TTTTCGG5450.016.14659529
ACGCTGA6450.016.12440311
GTATAGG1703.6379788E-1216.0020541
TACCGTA600.002441916416.0003687
AATCGCC600.002442488415.99980626
Produced by FastQC (version 0.11.5)