FastQCFastQC Report
Thu 2 Feb 2017
SRR4063083_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063083_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996668
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT52720.5289625030601965No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA41610.4174910802794913No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA36870.3699326154747619No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG35360.3547821340707236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33430.3354176114814562No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC30850.30953135848647695No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA20730.20799303278524042No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA18610.18672215823122645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18590.1865214896033584No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16430.1648492777936083No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA15340.15391283757479923No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA15090.15140447972644852No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT14860.1490967905059659No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14470.14518375226253877No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA13550.13595299538060818No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA13090.1313376169396429No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11290.11327744043151781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCT308.421701E-426.6636128
GATAACG453.535451E-421.33410311
TGAACGG604.7365465E-621.324475
GCGAAAA707.77949E-720.5721720
TAGGACC10700.020.0300644
TAAGCCG400.004482131520.00072115
ACGAACG400.004482131520.00072115
AACGGCT507.2005653E-419.1978027
ATGAACG601.1411822E-418.6598474
TGGCGAA1550.018.57758518
GGCGAAA953.790592E-818.52512419
CGAACGA450.008847631517.77841816
CCGATAA450.008847631517.7784189
TGTGGCG450.008847631517.77841816
CGCCTGG450.008847631517.77841820
CGATAAC450.008847631517.77841810
CGCCACT450.008847631517.77841822
CAACGAT450.0088501917.77752730
GCGCCGA450.00885274917.77663419
GAGGTCG450.00885530917.77574227
Produced by FastQC (version 0.11.5)