##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063083_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	996668
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.215257237114063	32.0	32.0	32.0	32.0	32.0
2	30.94139874060369	32.0	32.0	32.0	32.0	32.0
3	30.993949840869778	32.0	32.0	32.0	32.0	32.0
4	31.03852737320753	32.0	32.0	32.0	32.0	32.0
5	31.02842170110809	32.0	32.0	32.0	32.0	32.0
6	34.67719942849575	36.0	36.0	36.0	32.0	36.0
7	34.58120056026681	36.0	36.0	36.0	32.0	36.0
8	34.56085878145982	36.0	36.0	36.0	32.0	36.0
9	34.6452058258116	36.0	36.0	36.0	32.0	36.0
10	34.44797364819579	36.0	36.0	36.0	32.0	36.0
11	34.67635260688615	36.0	36.0	36.0	32.0	36.0
12	34.542486565235365	36.0	36.0	36.0	32.0	36.0
13	34.58725673945587	36.0	36.0	36.0	32.0	36.0
14	34.53472369936629	36.0	36.0	36.0	32.0	36.0
15	34.50957390023559	36.0	36.0	36.0	32.0	36.0
16	34.53444978668925	36.0	36.0	36.0	32.0	36.0
17	34.499304683204436	36.0	36.0	36.0	32.0	36.0
18	34.52801434379352	36.0	36.0	36.0	32.0	36.0
19	34.467809742060545	36.0	36.0	36.0	32.0	36.0
20	34.43501647489435	36.0	36.0	36.0	32.0	36.0
21	34.38981987983963	36.0	36.0	36.0	32.0	36.0
22	34.36633262029081	36.0	36.0	36.0	32.0	36.0
23	34.36896137931588	36.0	36.0	36.0	32.0	36.0
24	34.3398564015299	36.0	36.0	36.0	32.0	36.0
25	34.308122664718844	36.0	36.0	36.0	32.0	36.0
26	34.30899858327949	36.0	36.0	36.0	32.0	36.0
27	34.26577556417985	36.0	36.0	36.0	32.0	36.0
28	34.2201013777908	36.0	36.0	36.0	32.0	36.0
29	34.16862987474264	36.0	36.0	36.0	32.0	36.0
30	34.112564063459445	36.0	36.0	36.0	32.0	36.0
31	34.10902326552071	36.0	36.0	36.0	32.0	36.0
32	34.08435607444003	36.0	36.0	36.0	32.0	36.0
33	34.07976477623441	36.0	36.0	36.0	32.0	36.0
34	34.02647421207463	36.0	36.0	36.0	32.0	36.0
35	34.017788270517364	36.0	36.0	36.0	32.0	36.0
36	33.9441559275506	36.0	36.0	36.0	32.0	36.0
37	33.88168276697957	36.0	36.0	36.0	32.0	36.0
38	33.303117989139814	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	3.0
9	3.0
10	8.0
11	14.0
12	9.0
13	9.0
14	204.0
15	381.0
16	466.0
17	562.0
18	667.0
19	782.0
20	986.0
21	1284.0
22	1690.0
23	2436.0
24	3657.0
25	5528.0
26	8238.0
27	11869.0
28	17028.0
29	23485.0
30	32240.0
31	43846.0
32	59804.0
33	90761.0
34	213122.0
35	477583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.47116972670612	21.434541677215062	13.351155099596951	23.743133496481857
2	11.219278598599121	22.273913274817815	47.84241167204939	18.66439645453368
3	17.441216373517864	29.577054918236772	31.342068926762305	21.639659781483054
4	9.899761192831772	18.743352531556663	42.1112359776043	29.245650298007263
5	9.433240992227093	41.89624016848155	37.21764641550287	11.452872423788488
6	26.142284518870433	41.456858623509405	20.952576843773986	11.448280013846176
7	24.091472787327376	34.237880618219904	24.89163894095125	16.77900765350147
8	21.985543774030162	42.06603228369842	21.891630479278874	14.056793462992543
9	26.897202169234397	15.410293328249713	22.574285241279668	35.11821926123622
10	14.176292110480562	30.481586572806883	36.76424552306494	18.577875793647607
11	31.740161280450792	22.814651898511915	29.04814031820631	16.397046502830985
12	23.896311086807106	25.775434336542464	35.30921551019179	15.019039066458634
13	28.766926709630287	24.750176116053957	27.587400727745653	18.8954964465701
14	18.72453111155486	25.934139373584504	29.76843317856745	25.572896336293184
15	19.261302165190354	38.181577315180014	25.082647365220062	17.474473154409566
16	17.304722425424693	29.87748647072931	34.35325921711356	18.464531886732434
17	17.439288502800988	32.18286409335691	31.760457792488012	18.61738961135409
18	17.70493217927389	28.39168852435987	37.64898183620677	16.25439746015946
19	22.08026908214451	27.297849731028233	30.404496208506554	20.217384978320705
20	22.435658804275732	29.365312697860023	32.01331325174267	16.185715246121568
21	20.946268668697595	27.94404508181917	28.806688420892083	22.302997828591153
22	21.488396921900602	32.551332595483196	29.55601556910622	16.404254913509984
23	18.06210721406589	32.225730934297445	31.78692451184918	17.92523733978749
24	21.167364608766075	29.18240924996563	32.73413674446889	16.916089396799403
25	19.531402100632036	30.510747022967262	32.4737922770763	17.48405859932441
26	16.23956412960902	32.089542502682036	33.94002466774316	17.73086869996578
27	17.98719608256319	31.685380856337236	31.987416839760378	18.340006221339195
28	16.976539068606744	32.29402574571566	30.484460557118105	20.244974628559504
29	19.29583725945304	30.113162560923847	29.945681569364126	20.64531861025899
30	17.498286724548453	30.531521759852463	31.95301360887552	20.017177906723564
31	20.846841802654435	28.705685447578112	31.242217265354	19.20525548441346
32	17.97910876087458	32.016375841619926	31.63878826799386	18.365727129511637
33	16.81564492467132	32.25496354481289	33.71146517139858	17.217926359117207
34	18.357482105423713	31.74903741077799	33.40126900216852	16.492211481629774
35	17.5983349590363	32.49870545874929	32.594943060255375	17.308016521959033
36	16.67998009007635	31.575192074565877	33.642954054639894	18.10187378071788
37	17.53415255308773	31.61903271058985	29.992634648459997	20.854180087862424
38	18.164769203908513	34.03361850462676	29.911679179284643	17.889933112180085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	712.0
1	544.5
2	377.0
3	377.0
4	924.5
5	1472.0
6	1472.0
7	1685.5
8	1899.0
9	1923.0
10	1947.0
11	1947.0
12	2566.0
13	3185.0
14	4166.0
15	5147.0
16	5147.0
17	7570.5
18	9994.0
19	9994.0
20	12764.5
21	15535.0
22	18287.5
23	21040.0
24	21040.0
25	27555.5
26	34071.0
27	34071.0
28	45225.5
29	56380.0
30	73113.5
31	89847.0
32	89847.0
33	106304.5
34	122762.0
35	122762.0
36	126588.0
37	130414.0
38	129315.0
39	128216.0
40	128216.0
41	114733.5
42	101251.0
43	88356.0
44	75461.0
45	75461.0
46	68741.0
47	62021.0
48	62021.0
49	53916.0
50	45811.0
51	38006.0
52	30201.0
53	30201.0
54	25270.0
55	20339.0
56	20339.0
57	16879.0
58	13419.0
59	10836.5
60	8254.0
61	8254.0
62	7283.0
63	6312.0
64	5083.0
65	3854.0
66	3854.0
67	3275.5
68	2697.0
69	2697.0
70	2127.0
71	1557.0
72	1168.0
73	779.0
74	779.0
75	600.5
76	422.0
77	422.0
78	375.0
79	328.0
80	274.5
81	221.0
82	221.0
83	209.0
84	197.0
85	197.0
86	133.0
87	69.0
88	57.5
89	46.0
90	46.0
91	37.5
92	29.0
93	29.0
94	29.0
95	29.0
96	31.5
97	34.0
98	34.0
99	186.5
100	339.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1260198983011394
2	0.058093567767802314
3	0.01324412943929172
4	0.0048160470688333535
5	1.0033431393402818E-4
6	3.010029418020846E-4
7	0.0
8	4.013372557361127E-4
9	3.010029418020846E-4
10	4.013372557361127E-4
11	0.0019063519647465354
12	0.00331103235982293
13	0.01665549611304868
14	0.010936440218809072
15	0.026688927506451497
16	0.012541789241753523
17	0.023478229460562595
18	0.007023401975381973
19	0.009531759823732678
20	0.006722399033579888
21	0.008026745114722254
22	0.007926410800788226
23	0.010133765707336847
24	0.016755830426982705
25	0.020668868670409804
26	0.02257522063515634
27	0.00983276276553476
28	0.007525073545052113
29	0.013745801008961861
30	0.003913038243427099
31	0.007123736289316
32	0.007826076486854199
33	0.010234100021270875
34	0.013745801008961861
35	0.01765883925238896
36	0.018060176508125074
37	0.011036774532743099
38	0.007424739231118085
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	996668.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.47404491945001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.89262872994783	50.12852553306158
2	11.185359166334289	13.528478253301632
3	2.750231017305829	4.989527822382522
4	1.0165560830128622	2.459010329090399
5	0.508900009676308	1.5387621022336797
6	0.31659196321928723	1.1487357962916025
7	0.2064768585535851	0.8740543573297501
8	0.15181747253909025	0.734481332310905
9	0.10500539492676272	0.5715090872627073
>10	0.7287706798327023	8.543606898000007
>50	0.0766512660615966	3.2325179137447035
>100	0.05236669789846193	5.783428470853217
>500	0.005818521619226591	2.4169415878371425
>1k	0.0026598955973607275	3.5204044582710043
>5k	1.6624347483504547E-4	0.5300160580290927
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5272	0.5289625030601965	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	4161	0.4174910802794913	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	3687	0.3699326154747619	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	3536	0.3547821340707236	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3343	0.3354176114814562	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	3085	0.30953135848647695	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	2073	0.20799303278524042	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	1861	0.18672215823122645	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1859	0.1865214896033584	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1643	0.1648492777936083	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	1534	0.15391283757479923	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	1509	0.15140447972644852	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	1486	0.1490967905059659	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1447	0.14518375226253877	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	1355	0.13595299538060818	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1309	0.1313376169396429	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1129	0.11327744043151781	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.013372557361127E-4	0.0
11	0.0	0.0	0.0	4.013372557361127E-4	0.0
12	0.0	0.0	0.0	4.013372557361127E-4	0.0
13	0.0	0.0	0.0	4.013372557361127E-4	0.0
14	0.0	0.0	0.0	4.013372557361127E-4	0.0
15	0.0	0.0	0.0	4.013372557361127E-4	0.0
16	0.0	1.0033431393402818E-4	0.0	4.013372557361127E-4	0.0
17	0.0	1.0033431393402818E-4	0.0	4.013372557361127E-4	0.0
18	0.0	1.0033431393402818E-4	0.0	5.016715696701409E-4	0.0
19	0.0	1.0033431393402818E-4	0.0	6.020058836041691E-4	0.0
20	0.0	1.0033431393402818E-4	0.0	7.023401975381973E-4	0.0
21	0.0	1.0033431393402818E-4	0.0	0.0010033431393402818	0.0
22	0.0	1.0033431393402818E-4	0.0	0.0012040117672083381	0.0
23	0.0	1.0033431393402818E-4	0.0	0.0021070205926145918	0.0
24	0.0	1.0033431393402818E-4	0.0	0.00441470981309724	0.0
25	0.0	1.0033431393402818E-4	0.0	0.005618721580305578	0.0
26	0.0	1.0033431393402818E-4	0.0	0.006822733347513916	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGCT	30	8.421701E-4	26.663612	8
GATAACG	45	3.535451E-4	21.334103	11
TGAACGG	60	4.7365465E-6	21.32447	5
GCGAAAA	70	7.77949E-7	20.57217	20
TAGGACC	1070	0.0	20.030064	4
TAAGCCG	40	0.0044821315	20.000721	15
ACGAACG	40	0.0044821315	20.000721	15
AACGGCT	50	7.2005653E-4	19.197802	7
ATGAACG	60	1.1411822E-4	18.659847	4
TGGCGAA	155	0.0	18.577585	18
GGCGAAA	95	3.790592E-8	18.525124	19
CGAACGA	45	0.0088476315	17.778418	16
CCGATAA	45	0.0088476315	17.778418	9
TGTGGCG	45	0.0088476315	17.778418	16
CGCCTGG	45	0.0088476315	17.778418	20
CGATAAC	45	0.0088476315	17.778418	10
CGCCACT	45	0.0088476315	17.778418	22
CAACGAT	45	0.00885019	17.777527	30
GCGCCGA	45	0.008852749	17.776634	19
GAGGTCG	45	0.008855309	17.775742	27
>>END_MODULE