FastQCFastQC Report
Thu 2 Feb 2017
SRR4063083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996668
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT58240.5843470443517801No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT41650.4178924175352274No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG37760.37886236941489043No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA37750.37876203510095635No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA37230.3735446507763869No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC28730.28826048393246295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT23690.23769198970971278No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA21440.21511676907455643No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA20390.20458166611148346No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17550.17608672095421946No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT15200.15250815717972285No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA14640.14688943559941725No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14480.14528408657647282No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA14170.14217372284451793No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14080.14127071401911168No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA13730.13775901303142069No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA13400.13444798067159777No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG10730.10765871885121223No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT10500.10535102963072959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACCG200.003750484232.0048685
TTCATCG200.003754171531.99844728
TGTGCGC350.0020663322.85717810
GCGTCCT453.5338802E-421.3355089
GAGCCGC453.5374754E-421.33229616
TAGGACC10600.019.6246414
GCCGCCT507.197371E-419.19906818
TGTCCAC10300.018.95148710
CCGAGCC601.1384138E-418.66575814
ACTGTGC1500.018.1360918
AATGTCC11150.018.0834678
CCTCGAC450.00885195117.77691330
ACTGTTC5800.017.6578588
ATGTCCA11500.017.532229
GGGCGTC550.00136308317.4572017
TTAGGGC550.001363541517.4563263
GCCCGAG550.001364917617.45369712
TCCTCGC8100.017.1843529
AGGACCT19650.017.1018375
AAATGTC12000.016.935917
Produced by FastQC (version 0.11.5)