##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063083_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	996668
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14910682393736	32.0	32.0	32.0	32.0	32.0
2	31.34109954367954	32.0	32.0	32.0	32.0	32.0
3	31.41780813671152	32.0	32.0	32.0	32.0	32.0
4	31.51300834380155	32.0	32.0	32.0	32.0	32.0
5	31.453728824443044	32.0	32.0	32.0	32.0	32.0
6	34.98001541135062	36.0	36.0	36.0	36.0	36.0
7	35.00466153222538	36.0	36.0	36.0	36.0	36.0
8	34.934778682570325	36.0	36.0	36.0	36.0	36.0
9	35.05169424522509	36.0	36.0	36.0	36.0	36.0
10	34.937906103135646	36.0	36.0	36.0	36.0	36.0
11	35.08034270188267	36.0	36.0	36.0	36.0	36.0
12	35.022786926037554	36.0	36.0	36.0	36.0	36.0
13	35.0540561149751	36.0	36.0	36.0	36.0	36.0
14	35.01609262061188	36.0	36.0	36.0	36.0	36.0
15	34.98361841656399	36.0	36.0	36.0	36.0	36.0
16	35.00724815083859	36.0	36.0	36.0	36.0	36.0
17	35.01322105254709	36.0	36.0	36.0	36.0	36.0
18	35.00783510657511	36.0	36.0	36.0	36.0	36.0
19	34.97224652542271	36.0	36.0	36.0	36.0	36.0
20	34.960206407750626	36.0	36.0	36.0	36.0	36.0
21	34.93802148759667	36.0	36.0	36.0	36.0	36.0
22	34.921877696484685	36.0	36.0	36.0	36.0	36.0
23	34.888430249591636	36.0	36.0	36.0	32.0	36.0
24	34.877774745451845	36.0	36.0	36.0	32.0	36.0
25	34.854129961030154	36.0	36.0	36.0	32.0	36.0
26	34.7991798673179	36.0	36.0	36.0	32.0	36.0
27	34.7511859515907	36.0	36.0	36.0	32.0	36.0
28	34.70311377509863	36.0	36.0	36.0	32.0	36.0
29	34.68140744962214	36.0	36.0	36.0	32.0	36.0
30	34.61437409448282	36.0	36.0	36.0	32.0	36.0
31	34.60147511508346	36.0	36.0	36.0	32.0	36.0
32	34.58207748217059	36.0	36.0	36.0	32.0	36.0
33	34.58685439885699	36.0	36.0	36.0	32.0	36.0
34	34.53553941733857	36.0	36.0	36.0	32.0	36.0
35	34.50290869176095	36.0	36.0	36.0	32.0	36.0
36	34.44701144212516	36.0	36.0	36.0	32.0	36.0
37	34.38045066160446	36.0	36.0	36.0	32.0	36.0
38	34.035730052535044	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	11.0
22	54.0
23	152.0
24	418.0
25	1175.0
26	2701.0
27	5481.0
28	10128.0
29	16693.0
30	26314.0
31	38436.0
32	55051.0
33	87324.0
34	218896.0
35	533832.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.92349682909207	22.849702978245162	13.977883920687164	24.248916271975595
2	10.818447065622655	23.24996889636268	46.75890065698909	19.172683381025575
3	16.808104604542333	30.139825899898458	31.855843671112144	21.19622582444706
4	9.711759582930323	19.659003800663815	42.115629276750134	28.513607339655735
5	9.193742744567643	42.500253344396874	36.798047893629466	11.507956017406014
6	26.42708146251456	41.693464316567116	20.424575108837757	11.454879112080564
7	23.961262889209735	34.352998544147646	24.9469481782782	16.738790388364418
8	22.008749397977205	42.425750521753095	21.557131963397012	14.00836811687269
9	26.316386221566678	15.447156918816413	22.522500827789653	35.71395603182726
10	14.140672267389883	30.942841920540364	36.303362139632796	18.613123672436956
11	31.43863986642692	22.861446024916518	29.193123073465195	16.50679103519137
12	23.707488180570383	26.101173514845527	35.090160776349904	15.101177528234189
13	28.70316765780346	24.77056027740459	27.634104470199173	18.892167594592777
14	18.61502526418025	26.12825936018815	29.58307079187854	25.673644583753063
15	19.246830439022826	38.250450501069565	24.817090545698267	17.685628514209345
16	17.35592995862213	30.164809144068034	33.86152660665337	18.61773429065647
17	17.415528541098944	32.401662338913255	31.56377048325019	18.61903863673761
18	17.829106583135008	28.495246160205806	37.32125441972653	16.35439283693266
19	22.209201057925007	27.270665858640992	30.661664666669342	19.85846841676466
20	22.56197650571705	29.497184619151014	31.789623023915688	16.151215851216254
21	20.820494708864643	27.873101045785603	29.088853147540757	22.217551097808997
22	21.546313864109077	32.3889523782768	29.64059209344746	16.424141664166665
23	18.026664847271107	32.29721431810793	31.65417170010475	18.02194913451621
24	21.266158841259074	29.18905794105961	32.41841816934024	17.12636504834107
25	19.652100450802525	30.708350933661894	32.1093203647758	17.530228250759784
26	16.289476535817343	32.09915438240216	33.64500515718374	17.966363924596756
27	18.246798332042367	31.684572997226763	31.765743457199385	18.302885213531486
28	17.052418658971693	32.2381174072008	30.63989212054566	20.069571813281854
29	19.24903779392937	29.891297804283873	30.200026488258878	20.659637913527877
30	17.534424703110766	30.255009692294728	32.08229821766125	20.128267386933263
31	20.597932310458447	28.67293823018297	31.434238883961356	19.294890575397222
32	18.0977015415364	31.850325283845777	31.636211857910556	18.41576131670727
33	16.864392154659324	32.27825113277441	33.5048381206179	17.35251859194837
34	18.48459065606601	31.633402497120404	33.25911938579346	16.62288746102012
35	17.861012894966027	32.468986663562994	32.184137546304285	17.485862895166697
36	16.741783623031942	31.485911055637384	33.474838160751624	18.29746716057905
37	17.670879370061044	31.4255097986491	29.992635461357242	20.910975369932615
38	18.42522971587272	33.41791532970925	30.06915054792679	18.08770440649124
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	388.0
1	308.5
2	229.0
3	229.0
4	787.5
5	1346.0
6	1346.0
7	1593.0
8	1840.0
9	1887.5
10	1935.0
11	1935.0
12	2555.0
13	3175.0
14	4113.5
15	5052.0
16	5052.0
17	7810.0
18	10568.0
19	10568.0
20	13130.0
21	15692.0
22	18520.0
23	21348.0
24	21348.0
25	27634.5
26	33921.0
27	33921.0
28	44961.0
29	56001.0
30	72823.0
31	89645.0
32	89645.0
33	105980.0
34	122315.0
35	122315.0
36	126324.5
37	130334.0
38	129937.5
39	129541.0
40	129541.0
41	115590.5
42	101640.0
43	88298.5
44	74957.0
45	74957.0
46	68709.0
47	62461.0
48	62461.0
49	54491.5
50	46522.0
51	38453.0
52	30384.0
53	30384.0
54	25321.0
55	20258.0
56	20258.0
57	16687.0
58	13116.0
59	10650.0
60	8184.0
61	8184.0
62	7145.0
63	6106.0
64	4926.0
65	3746.0
66	3746.0
67	3223.5
68	2701.0
69	2701.0
70	2089.5
71	1478.0
72	1083.0
73	688.0
74	688.0
75	527.5
76	367.0
77	367.0
78	325.5
79	284.0
80	239.0
81	194.0
82	194.0
83	173.5
84	153.0
85	153.0
86	104.0
87	55.0
88	39.0
89	23.0
90	23.0
91	17.5
92	12.0
93	9.5
94	7.0
95	7.0
96	4.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010836105904875045
2	0.0
3	0.0
4	0.0
5	1.0033431393402818E-4
6	1.0033431393402818E-4
7	1.0033431393402818E-4
8	0.002809360790152789
9	0.003812703929493071
10	0.00110367745327431
11	0.006020058836041691
12	4.013372557361127E-4
13	1.0033431393402818E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0033431393402818E-4
22	1.0033431393402818E-4
23	0.0
24	0.0
25	1.0033431393402818E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.0066862786805636E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	996668.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.79341570387897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.78445276342656	48.08387329434391
2	11.906651364825539	14.000654066666915
3	2.94208961377881	5.189264931028869
4	1.0926873080862889	2.569712765546786
5	0.5386305526339875	1.5833964995910044
6	0.32690140215605773	1.153179001868521
7	0.21409973813555344	0.8811358434406656
8	0.16405021181896112	0.7716057839825263
9	0.11658268670921446	0.6168864927212938
>10	0.7676755259277964	8.777475952344762
>50	0.08140640819019447	3.355951796304988
>100	0.055347865585481604	6.0077755225393945
>500	0.006168802074920323	2.522272299348842
>1k	0.0030844010374601613	3.900072106262676
>5k	1.713556131922312E-4	0.5867436440089332
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5824	0.5843470443517801	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4165	0.4178924175352274	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	3776	0.37886236941489043	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	3775	0.37876203510095635	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	3723	0.3735446507763869	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	2873	0.28826048393246295	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2369	0.23769198970971278	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	2144	0.21511676907455643	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	2039	0.20458166611148346	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1755	0.17608672095421946	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	1520	0.15250815717972285	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	1464	0.14688943559941725	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1448	0.14528408657647282	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	1417	0.14217372284451793	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1408	0.14127071401911168	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1373	0.13775901303142069	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	1340	0.13444798067159777	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	1073	0.10765871885121223	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	1050	0.10535102963072959	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.0033431393402818E-4	0.0
8	0.0	0.0	0.0	1.0033431393402818E-4	0.0
9	0.0	0.0	0.0	1.0033431393402818E-4	0.0
10	0.0	0.0	0.0	6.020058836041691E-4	0.0
11	0.0	0.0	0.0	6.020058836041691E-4	0.0
12	0.0	0.0	0.0	6.020058836041691E-4	0.0
13	0.0	0.0	0.0	6.020058836041691E-4	0.0
14	0.0	0.0	0.0	6.020058836041691E-4	0.0
15	0.0	0.0	0.0	6.020058836041691E-4	0.0
16	0.0	0.0	0.0	6.020058836041691E-4	0.0
17	0.0	0.0	0.0	7.023401975381973E-4	0.0
18	0.0	0.0	0.0	8.026745114722254E-4	0.0
19	0.0	0.0	0.0	9.030088254062536E-4	0.0
20	0.0	0.0	0.0	0.00110367745327431	0.0
21	0.0	0.0	0.0	0.0014046803950763945	0.0
22	0.0	0.0	0.0	0.001705683336878479	0.0
23	0.0	0.0	0.0	0.002709026476218761	0.0
24	0.0	0.0	0.0	0.005016715696701409	0.0
25	0.0	0.0	0.0	0.006220727463909747	0.0
26	0.0	0.0	0.0	0.007224070603250029	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACCG	20	0.0037504842	32.004868	5
TTCATCG	20	0.0037541715	31.998447	28
TGTGCGC	35	0.00206633	22.857178	10
GCGTCCT	45	3.5338802E-4	21.335508	9
GAGCCGC	45	3.5374754E-4	21.332296	16
TAGGACC	1060	0.0	19.624641	4
GCCGCCT	50	7.197371E-4	19.199068	18
TGTCCAC	1030	0.0	18.951487	10
CCGAGCC	60	1.1384138E-4	18.665758	14
ACTGTGC	150	0.0	18.136091	8
AATGTCC	1115	0.0	18.083467	8
CCTCGAC	45	0.008851951	17.776913	30
ACTGTTC	580	0.0	17.657858	8
ATGTCCA	1150	0.0	17.53222	9
GGGCGTC	55	0.001363083	17.457201	7
TTAGGGC	55	0.0013635415	17.456326	3
GCCCGAG	55	0.0013649176	17.453697	12
TCCTCGC	810	0.0	17.18435	29
AGGACCT	1965	0.0	17.101837	5
AAATGTC	1200	0.0	16.93591	7
>>END_MODULE