FastQCFastQC Report
Thu 2 Feb 2017
SRR4063082_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063082_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706572
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT21620.3059843865876372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT14680.20776368154979252No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14150.20026267669819917No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12520.17719354856971406No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12190.17252311158664652No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11780.16672044745616865No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11630.1645975215547743No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11620.164455993161348No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11460.1621915388665274No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11110.15723804509660727No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10860.15369983526095005No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10660.15086926739242426No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10340.146340358802783No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG9910.1402546378854526No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9860.13954699591832112No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA9770.13827324037748454No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT8950.12666791211652884No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT8830.12496957139541336No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG8300.11746856654382001No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA8270.11704398136354116No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT8260.11690245297011487No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT8240.1166193961832623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT8040.1137888283147365No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG7920.11209048759362103No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC7920.11209048759362103No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC7820.11067520365935814No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG7820.11067520365935814No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC7560.10699546543027462No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7480.1058632382828643No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT7470.10572170988943802No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT7130.10090974451294418No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG7080.10020210254581274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACC751.5111054E-619.1943953
AACGGAC601.1362361E-418.66914415
TCTAGCG2400.018.6651828
CATCGTT2300.018.08551628
CTAGCGG2500.017.91857329
GTATTAT450.00881861417.7876951
TAGGCTG550.001365963317.4506865
ATCGTTT2400.017.33195329
AAGACGG3100.017.028495
TAGCGGC2650.016.90431430
GTCCTAA1151.8246283E-816.7049641
GTATTAG2500.016.6492821
CGGTCCA2900.016.5539210
AACCGCG1456.91216E-1116.5504067
GTTATTC1456.91216E-1116.5468923
TGGCGAT2050.016.38893930
CGTTTAT2550.016.31242831
TCCTACA805.6095676E-516.0021252
TTAGGTA703.6961175E-415.99872731
TAGGTAG703.6961175E-415.99872732
Produced by FastQC (version 0.11.5)