##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063082_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	706572
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.245224832005796	32.0	32.0	32.0	32.0	32.0
2	30.887934987517195	32.0	32.0	32.0	32.0	32.0
3	30.94736417520083	32.0	32.0	32.0	32.0	32.0
4	31.00655417989957	32.0	32.0	32.0	32.0	32.0
5	30.95115005972498	32.0	32.0	32.0	32.0	32.0
6	34.594560780783844	36.0	36.0	36.0	32.0	36.0
7	34.506960366388704	36.0	36.0	36.0	32.0	36.0
8	34.49117428938594	36.0	36.0	36.0	32.0	36.0
9	34.600186534422534	36.0	36.0	36.0	32.0	36.0
10	34.35650577718902	36.0	36.0	36.0	32.0	36.0
11	34.600947957179166	36.0	36.0	36.0	32.0	36.0
12	34.414720651257056	36.0	36.0	36.0	32.0	36.0
13	34.512066710823525	36.0	36.0	36.0	32.0	36.0
14	34.413354902260494	36.0	36.0	36.0	32.0	36.0
15	34.36816630152341	36.0	36.0	36.0	32.0	36.0
16	34.38203466879526	36.0	36.0	36.0	32.0	36.0
17	34.29974723028934	36.0	36.0	36.0	32.0	36.0
18	34.31952865383853	36.0	36.0	36.0	32.0	36.0
19	34.304854990008096	36.0	36.0	36.0	32.0	36.0
20	34.28138250595835	36.0	36.0	36.0	32.0	36.0
21	34.25405761903953	36.0	36.0	36.0	32.0	36.0
22	34.237757793968626	36.0	36.0	36.0	32.0	36.0
23	34.176768963389435	36.0	36.0	36.0	32.0	36.0
24	34.164392305384304	36.0	36.0	36.0	32.0	36.0
25	34.13999281035761	36.0	36.0	36.0	32.0	36.0
26	34.081043403927694	36.0	36.0	36.0	32.0	36.0
27	34.07617907304564	36.0	36.0	36.0	32.0	36.0
28	34.06000237767701	36.0	36.0	36.0	32.0	36.0
29	34.02290637047604	36.0	36.0	36.0	32.0	36.0
30	33.99454266514948	36.0	36.0	36.0	32.0	36.0
31	34.00529599248201	36.0	36.0	36.0	32.0	36.0
32	33.97201700605176	36.0	36.0	36.0	32.0	36.0
33	33.91389412543945	36.0	36.0	36.0	32.0	36.0
34	33.918440017436296	36.0	36.0	36.0	32.0	36.0
35	33.8495241815413	36.0	36.0	36.0	32.0	36.0
36	33.82312772088336	36.0	36.0	36.0	32.0	36.0
37	33.81629614533268	36.0	36.0	36.0	32.0	36.0
38	33.195559688184645	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	1.0
8	1.0
9	1.0
10	6.0
11	5.0
12	2.0
13	4.0
14	146.0
15	269.0
16	315.0
17	380.0
18	440.0
19	608.0
20	757.0
21	958.0
22	1431.0
23	2120.0
24	3163.0
25	4599.0
26	6655.0
27	9527.0
28	13544.0
29	18114.0
30	24587.0
31	32693.0
32	44459.0
33	66846.0
34	150591.0
35	324348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.71693755225234	17.399781780051295	11.066160320811665	25.817120346884696
2	16.81340109976478	20.206584710635546	36.86109628110702	26.118917908492662
3	18.643520799008034	24.48193569208296	28.74365158901364	28.13089191989537
4	12.399688627839502	15.271813742834903	36.328639162125825	35.99985846719977
5	14.507813086016833	36.669067935140276	33.43089371061082	15.392225268232067
6	33.718715148633116	35.97552691020873	16.974066337188567	13.33169160396959
7	29.963301635645955	30.748219216469398	21.23084587394614	18.0576332739385
8	28.436713187247104	32.15354763653656	19.869397044025426	19.54034213219091
9	27.623448490595408	14.032721457180465	18.90060432795052	39.4432257242736
10	16.186812611365628	25.93249916144071	31.09491075889262	26.785777468301042
11	37.88402667010115	21.080475940438095	22.041471412861487	18.994025976599264
12	25.006404313889224	24.153250631585653	28.117980907360362	22.722364147164765
13	29.30394842070249	18.949871547166605	25.61285802246332	26.13332200966758
14	23.86700277844223	20.202233227791215	24.768899564196452	31.161864429570095
15	25.435761536904362	26.938011656169884	22.58414766558089	25.042079141344864
16	26.12095785668385	25.472120074622005	23.760145167545655	24.64677690114849
17	23.893871703839597	25.720951038180818	25.445480643462602	24.93969661451698
18	25.573113858478347	23.81297458724621	26.714883197576906	23.899028356698533
19	25.77959576515881	24.99759384023099	25.35271471437468	23.87009568023552
20	25.708705159687774	23.879213343995698	25.253526010742565	25.158555485573963
21	27.384605368192805	24.048167669688	24.125305010954918	24.44192195116428
22	25.967621856803568	24.218641635268906	24.763278350690562	25.05045815723696
23	24.032854360577034	23.777656996625677	25.732896256539167	26.456592386258126
24	24.74726743083295	25.341907320539352	25.103541794648947	24.80728345397875
25	25.022789812812125	24.21339757890088	25.19590745920482	25.567905149082183
26	24.36441877583376	25.262159560613785	25.91118849442274	24.46223316912972
27	25.431340744326402	24.741478631825377	24.81522056055189	25.011960063296335
28	24.14692590438097	24.357956387033585	26.027234396694848	25.4678833118906
29	24.209414835343708	24.84975272539922	25.940195498385	25.000636940872074
30	24.241956759266074	24.963060252832047	26.366487582017562	24.428495405884316
31	24.957645530241162	24.90782506790161	24.541106835169284	25.593422566687945
32	24.351438377976717	24.842433034924454	24.648101624146353	26.15802696295248
33	23.880387874601745	24.419511490877078	25.61750106508407	26.08259956943711
34	25.010863395805526	24.659554622003366	25.881916656876637	24.44766532531447
35	26.24283233140497	24.36971935153743	25.520085042415047	23.867363274642557
36	24.299794329845103	25.39254972957135	25.01716277101254	25.29049316957101
37	25.68084573347053	25.180216585445947	24.857645528519726	24.281292152563797
38	24.41612776756358	24.907258401235328	25.446794417245904	25.229819413955184
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	345.0
1	272.0
2	199.0
3	199.0
4	453.5
5	708.0
6	708.0
7	798.0
8	888.0
9	840.5
10	793.0
11	793.0
12	1031.5
13	1270.0
14	1601.0
15	1932.0
16	1932.0
17	2666.5
18	3401.0
19	3401.0
20	4043.5
21	4686.0
22	4979.0
23	5272.0
24	5272.0
25	6075.5
26	6879.0
27	6879.0
28	9005.5
29	11132.0
30	13507.0
31	15882.0
32	15882.0
33	20788.0
34	25694.0
35	25694.0
36	28162.0
37	30630.0
38	35118.5
39	39607.0
40	39607.0
41	41166.0
42	42725.0
43	48872.0
44	55019.0
45	55019.0
46	56915.0
47	58811.0
48	58811.0
49	61749.0
50	64687.0
51	64621.5
52	64556.0
53	64556.0
54	60403.0
55	56250.0
56	56250.0
57	55451.5
58	54653.0
59	48685.0
60	42717.0
61	42717.0
62	42328.0
63	41939.0
64	34496.5
65	27054.0
66	27054.0
67	22746.0
68	18438.0
69	18438.0
70	14851.5
71	11265.0
72	8777.0
73	6289.0
74	6289.0
75	4699.0
76	3109.0
77	3109.0
78	3284.5
79	3460.0
80	2713.0
81	1966.0
82	1966.0
83	2081.5
84	2197.0
85	2197.0
86	1447.0
87	697.0
88	612.5
89	528.0
90	528.0
91	374.5
92	221.0
93	203.0
94	185.0
95	185.0
96	153.0
97	121.0
98	121.0
99	244.0
100	367.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12199747513346128
2	0.059583453632467746
3	0.013586725768923762
4	0.0031136246553783624
5	1.415283934262892E-4
6	0.0
7	1.415283934262892E-4
8	4.245851802788676E-4
9	2.830567868525784E-4
10	4.245851802788676E-4
11	0.0018398691145417592
12	0.002405982688246916
13	0.01231297022808716
14	0.00863323199900364
15	0.023352184915337717
16	0.011039214687250557
17	0.020380088653385642
18	0.0052365505567726995
19	0.007359476458167037
20	0.0052365505567726995
21	0.005661135737051567
22	0.006510306097609302
23	0.00849170360557735
24	0.013303668982071184
25	0.016417293637449544
26	0.018681747932270173
27	0.007642533245019616
28	0.0055196073436252786
29	0.009906987539840243
30	0.002264454294820627
31	0.004953493769920121
32	0.006651834491035592
33	0.007784061638445905
34	0.010048515933266531
35	0.014294367736055207
36	0.014294367736055207
37	0.00863323199900364
38	0.0055196073436252786
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	706572.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.69775982895332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53620638590951	41.33703008716811
2	11.219638355017846	11.376210613808155
3	3.1476229343035915	4.787322946662864
4	1.315383642800576	2.667480160225493
5	0.647074008481115	1.6402601336766836
6	0.3848960532391543	1.1708020599738407
7	0.28012294970041535	0.9941124218542742
8	0.19332694507991924	0.7840994424101596
9	0.13803113490688276	0.6298082393784298
>10	0.8518884517845342	8.434676721760548
>50	0.12817938549889824	4.643872785661461
>100	0.14052047400731013	14.633927137365403
>500	0.013463039425847676	4.559549072124796
>1k	0.003646239844500412	2.34084817792978
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2162	0.3059843865876372	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1468	0.20776368154979252	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1415	0.20026267669819917	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1252	0.17719354856971406	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1219	0.17252311158664652	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1178	0.16672044745616865	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1163	0.1645975215547743	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1162	0.164455993161348	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1146	0.1621915388665274	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1111	0.15723804509660727	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1086	0.15369983526095005	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1066	0.15086926739242426	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1034	0.146340358802783	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	991	0.1402546378854526	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	986	0.13954699591832112	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	977	0.13827324037748454	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	895	0.12666791211652884	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	883	0.12496957139541336	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	830	0.11746856654382001	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	827	0.11704398136354116	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	826	0.11690245297011487	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	824	0.1166193961832623	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	804	0.1137888283147365	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	792	0.11209048759362103	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	792	0.11209048759362103	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	782	0.11067520365935814	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	782	0.11067520365935814	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	756	0.10699546543027462	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	748	0.1058632382828643	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	747	0.10572170988943802	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	713	0.10090974451294418	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	708	0.10020210254581274	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.415283934262892E-4
7	0.0	0.0	0.0	0.0	1.415283934262892E-4
8	0.0	0.0	0.0	0.0	1.415283934262892E-4
9	0.0	0.0	0.0	0.0	1.415283934262892E-4
10	0.0	0.0	0.0	0.0	1.415283934262892E-4
11	1.415283934262892E-4	0.0	0.0	0.0	1.415283934262892E-4
12	1.415283934262892E-4	0.0	0.0	0.0	2.830567868525784E-4
13	1.415283934262892E-4	0.0	0.0	0.0	2.830567868525784E-4
14	1.415283934262892E-4	0.0	0.0	0.0	2.830567868525784E-4
15	1.415283934262892E-4	0.0	0.0	0.0	7.07641967131446E-4
16	1.415283934262892E-4	0.0	0.0	4.245851802788675E-4	7.07641967131446E-4
17	1.415283934262892E-4	0.0	0.0	4.245851802788675E-4	7.07641967131446E-4
18	1.415283934262892E-4	0.0	0.0	4.245851802788675E-4	7.07641967131446E-4
19	1.415283934262892E-4	0.0	0.0	5.661135737051568E-4	7.07641967131446E-4
20	1.415283934262892E-4	0.0	0.0	7.07641967131446E-4	7.07641967131446E-4
21	1.415283934262892E-4	0.0	0.0	0.0011322271474103135	7.07641967131446E-4
22	1.415283934262892E-4	0.0	0.0	0.0012737555408366026	7.07641967131446E-4
23	1.415283934262892E-4	0.0	0.0	0.002405982688246916	7.07641967131446E-4
24	1.415283934262892E-4	0.0	0.0	0.0036797382290835188	7.07641967131446E-4
25	1.415283934262892E-4	0.0	0.0	0.004104323409362386	7.07641967131446E-4
26	1.415283934262892E-4	0.0	0.0	0.0052365505567726995	7.07641967131446E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACC	75	1.5111054E-6	19.194395	3
AACGGAC	60	1.1362361E-4	18.669144	15
TCTAGCG	240	0.0	18.66518	28
CATCGTT	230	0.0	18.085516	28
CTAGCGG	250	0.0	17.918573	29
GTATTAT	45	0.008818614	17.787695	1
TAGGCTG	55	0.0013659633	17.450686	5
ATCGTTT	240	0.0	17.331953	29
AAGACGG	310	0.0	17.02849	5
TAGCGGC	265	0.0	16.904314	30
GTCCTAA	115	1.8246283E-8	16.704964	1
GTATTAG	250	0.0	16.649282	1
CGGTCCA	290	0.0	16.55392	10
AACCGCG	145	6.91216E-11	16.550406	7
GTTATTC	145	6.91216E-11	16.546892	3
TGGCGAT	205	0.0	16.388939	30
CGTTTAT	255	0.0	16.312428	31
TCCTACA	80	5.6095676E-5	16.002125	2
TTAGGTA	70	3.6961175E-4	15.998727	31
TAGGTAG	70	3.6961175E-4	15.998727	32
>>END_MODULE