FastQCFastQC Report
Thu 2 Feb 2017
SRR4063082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706572
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT25560.36174657359759516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18650.2639504537400293No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15090.21356634568027036No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14410.20394241492728268No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13210.18695900771612803No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13200.18681747932270173No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12170.17224005479979393No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11820.16728656102987383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11450.1620500104731011No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11240.15907791421114903No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10990.1555397043754918No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10650.15072773899899797No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10580.14973704024501394No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10380.14690647237648818No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10280.14549118844222528No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9580.13558420090238504No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9420.1333197466075644No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG9410.13317821821413814No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA9340.13218751946015408No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG9340.13218751946015408No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9190.13006459355875977No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC8960.1268094405099551No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT8470.11987454923206695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.1176100949372463No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT8140.1152041122489994No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT8100.11463799867529424No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC7950.1125150727738999No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT7740.10954297651194782No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT7730.10940144811852152No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG7650.10826922097111123No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG7510.10628782346314318No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC7390.10458948274202771No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT7340.10388184077489626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC957.2759576E-1121.8971581
TTTAGAC604.7117956E-621.3356913
GTATAGA400.00447893220.0022131
TCTAGCG2350.019.74406828
CTAGCGG2350.019.74406829
TAGCGGC2400.019.33273530
TATACAC1301.2732926E-1118.463583
TGTAGGA1850.018.1628862
TACCGTC2150.017.8611767
TCCATAC450.00884135917.7797453
CATATAG450.00884135917.7797451
CTAAGCT450.00884135917.7797454
TAACTTT904.3557884E-717.7797456
TCTATAC450.00884496417.7784862
CCGTCGT2000.017.6007029
ACCGTCG2000.017.6007028
CGTCGTA2000.017.59945710
CATTAGT550.001363577117.455242
CCCTAGA652.097188E-417.2326741
AGCGTAT1404.0017767E-1117.143548
Produced by FastQC (version 0.11.5)